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Method for extracting sophoricoside from sophora fruits

A technology of sophorin and sophora, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of low product content, large amount of toxic organic solvents, etc., and achieve large preparation volume and easy operation Simple, good leaching effect

Inactive Publication Date: 2012-07-04
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Liu Xiaohua's master's thesis "Research on Sophora japonicus and revision of Sophora japonicus content determination item standard in the 2005 edition of Chinese Pharmacopoeia". , and finally use dimethylformamide to recrystallize to obtain the product. Although this method is simple to operate, it consumes a large amount of toxic organic solvent and the product content is not high

Method used

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  • Method for extracting sophoricoside from sophora fruits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Pulverize the medicinal material Sophora japonica, weigh 1kg, add 10L of pH 9.0 alkaline dilute alcohol solution (20% ethanol) containing 20g of borax, heat and reflux for extraction twice, each time for 2.5 hours, combine the extracts, add hydrochloric acid to adjust to medium Then add the extract to the AB-8 macroporous resin column for adsorption, wash with water until the sugar reaction is negative, then elute with 5BV of 65% ethanol, the elution flow rate is 0.5BV / h, collect the eluate, Concentrate to extract, add 120ml of ethyl acetate to dissolve, filter, then dissolve the insoluble matter with 100ml of acetone, filter, and dissolve the filter cake with 70% ethanol for 3 times to obtain 37.1g of sophorin, with a content of 98.3%.

Embodiment 2

[0022] Pulverize the medicinal material Sophora japonica, weigh 1kg, add 8L of pH 9.0 alkaline dilute alcohol solution (15% ethanol) containing 18g of borax, heat and reflux for extraction 3 times, each time for 2 hours, combine the extracts, add hydrochloric acid to adjust to medium Then add the extract to the HPD450 macroporous resin column for adsorption, wash with water until the sugar reaction is negative, then elute with 6BV of 70% ethanol, the elution flow rate is 2BV / h, collect the eluate, concentrate to Ointment, add 100ml ethyl acetate to dissolve, filter, insoluble matter is dissolved with 80ml of acetone again, filter, and filter cake dissolves and crystallizes with 80% ethanol 4 times to obtain sophorin 33.6g, content 98.7%.

Embodiment 3

[0024] Pulverize the medicinal material Sophora japonica, weigh 2kg, add 22L of pH 8.5 alkaline dilute alcohol solution (25% ethanol) containing 28g of borax, heat and reflux to extract 3 times, each time for 3 hours, combine the extracts, add hydrochloric acid to adjust to medium Then add the extract to the HPD450 type macroporous resin column for adsorption, first wash with water until the sugar reaction is negative, then elute with 4BV of 50% ethanol, the elution flow rate is 1.5BV / h, collect the eluate, and concentrate to Extract, add 250ml ethyl acetate to dissolve, filter, dissolve insoluble matter with 180ml of acetone, filter, and dissolve the filter cake with 70% ethanol to crystallize twice to obtain 70.3g of sophorin, content 97.9%.

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Abstract

The invention provides a method for extracting sophoricoside from sophora fruits, which is characterized by enabling sophora fruit medicinal materials to be smashed, adding 8-12 times amount of alkalinity thin alcohol liquid containing sodium borate to heat and reflux for extracting 2-3 times, adjusting extracting solution to be neutral, passing macroporous resin column, eluting by using ethanol water, enabling eluent to be concentrated to extract, adding defined amount ethyl acetate for dissolving, filtering, dissolving undissolved substance by using acetone, filtering, obtaining filter cakes to conduct backflow dissolving by using 70-90% ethanol, and crystallizing 2-4 times to obtain products. The products manufactured by the method are high in product contents, good in repeatability and large in handling capacity of macroporous resin and have industrialization significances.

Description

Technical field: [0001] The invention belongs to the field of separation of natural products, and in particular relates to a method for extracting sophorin from Sophora japonica. Background technique: [0002] Sophora japonica L. is a commonly used traditional Chinese medicinal material in my country, the dried and mature fruit of Sophora japonica L., a leguminous plant. Medicinal products call the fruit Huaijiao. Its medicinal use was first recorded in "Shen Nong's Materia Medica" in the Eastern Han Dynasty, and it was listed as the top grade. Sophora japonica is bitter in the mouth, cold in nature. Return liver, large intestine channel. It has the functions of clearing away heat and purging fire, cooling blood and stopping bleeding. For intestinal heat with blood in the stool, hemorrhoids and hemorrhage, headache due to liver heat, dizziness and red eyes. The main components of Sophora japonicus are flavonoids, alkaloids, triterpenoids, amino acids and sugars, among w...

Claims

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Application Information

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IPC IPC(8): C07H15/26C07H1/08
Inventor 李法庆刘东锋
Owner 苏州宝泽堂医药科技有限公司
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