Aspergillus flavus CS05 with high yield of aflatoxin B1 and application thereof

A technology of aflatoxin and Aspergillus flavus, which is applied to Aspergillus flavus CS05 and its application field, and can solve the problem of small toxin production

Inactive Publication Date: 2016-11-30
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the standard toxin-producing strain Aspergillus flavus NRRL3357 commonly used in China has a small amount of toxin production.

Method used

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  • Aspergillus flavus CS05 with high yield of aflatoxin B1 and application thereof
  • Aspergillus flavus CS05 with high yield of aflatoxin B1 and application thereof
  • Aspergillus flavus CS05 with high yield of aflatoxin B1 and application thereof

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Experimental program
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Effect test

Embodiment 1

[0020] Isolation, purification, identification of embodiment 1 Aspergillus flavus CS05

[0021] 1. Isolation and purification of strains

[0022] The method refers to "Identification of Common Toxigenic Molds in Microbiological Examination of Food Hygiene" (GB / T 4789.16-2003) and "Standard Atlas of Fungi for Grain and Oil Inspection and Stored Grain Part 1: Aspergillus" (GB / T 26628.1-2011). Use the dilution separation method to isolate the mold in the sample, and then use the mycelia tip picking method or spot planting method to purify the obtained strains, number them and save them for future use. Cottonseed samples were pulverized and prepared into a spore suspension. After dilution, the appropriate dilution suspension was selected and inoculated into Chapeauer's agar medium by coating method, and cultured continuously at 28°C for 5-7 days. In order to separate the target mold from other molds and bacteria, further isolation and purification are required. When the mold gro...

Embodiment 2

[0038] The toxin-producing ability analysis of embodiment 2 Aspergillus flavus CS05

[0039] Inoculate 2uL of spore suspension in the center of potato dextrose agar (PDA) medium (25mL), culture upside down at 30°C for 7 days, and measure AFB by high performance liquid chromatography 1 content. Accurately weigh 20 g (accurate to 0.01 g) of the prepared samples (corn, wheat, soybean, soybean meal, cottonseed and cotton meal), put them into a series of 150 mL Erlenmeyer flasks, and autoclave at 121 °C for 20 min. Weigh non-sterile samples in the same manner. Inoculate the spore suspension of Aspergillus flavus in the Erlenmeyer flask containing the sample in the aseptic operating table to ensure that the final spore concentration is 10 6 pcs / g. The water content of the sample was adjusted to 18% with sterile physiological water. Fully mix the sample in each Erlenmeyer flask with a sterile glass rod, so that the Aspergillus flavus spore suspension is evenly distributed and sea...

Embodiment 3

[0050] Comparison of the toxin-producing ability of embodiment 3 Aspergillus flavus CS05 and standard toxin-producing strain NRRL3357

[0051] Aspergillus flavus CS05 and standard toxin-producing bacterial strain NRRL3357 were inoculated into pulverized corn, wheat, soybean, soybean meal, cottonseed and cotton meal respectively by the method in above-mentioned embodiment 2, after cultivating 10d in the constant temperature and humidity incubator, yellow yellow AFB of Aspergillus CS05 in the above-mentioned feed ingredients and by-products 1 The yields were 11.313mg / kg, 0.025mg / kg, 0.461mg / kg, 0.024mg / kg, 0.089mg / kg, 0.021mg / kg, all higher than the AFB of the standard toxin-producing strain NRRL3357 1 Yield (1.528 mg / kg, 0.004 mg / kg, 0.094 mg / kg, 0.018 mg / kg, 0.008 mg / kg, 0.008 mg / kg).

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Abstract

The invention discloses an Aspergillus flavus CS05, which is preserved in China Center for Type Culture Collection with a preservation number of CCTCC NO:M2016271. The Aspergillus flavus provided by the invention has strong aflatoxin B1 (AFB1) production ability. The Aspergillus flavus has high toxin yield in PDA medium, corn, wheat, soybean, soybean meal, cottonseed, cottonseed meal and other feed raw materials and by-products, and can be used as an AFB1 toxin production strain in AFB1 production, also can be used for the technical research of AFB1 prevention and control, and provides an experimental material for AFB1 biosynthesis mechanism, synthesis condition and removal method sturdy.

Description

technical field [0001] The invention relates to Aspergillus flavus, in particular to a high-yield aflatoxin B 1 The Aspergillus flavus CS05 and its application. Background technique [0002] Mold is a common name for some filamentous fungi. Mycotoxins (Mycotoxins) are toxic secondary metabolites produced by mold during growth and reproduction, and are commonly found in feed and feed ingredients. Aflatoxin (Afltoxin, AFT) is one of the more common mycotoxins, mainly produced by Aspergillus flavus, Aspergillus parasiticus and other molds with carcinogenic, teratogenic and mutagenic secondary metabolites. Aflatoxin B 1 (Aflatoxin B 1 ,AFB 1 ) are the most common and most toxic. Aflatoxins widely exist in feed, food raw materials and their products, and are extremely harmful to human and livestock health. Crops or feed raw materials may be polluted by mold or mycotoxins due to improper production management or environmental conditions from field growth to harvest, storage,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/67
CPCC12N1/145C12R2001/67
Inventor 齐德生杨双琦张妮娅孙铝辉宋文静刘婕高鑫张家才李翀
Owner HUAZHONG AGRI UNIV
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