Xylanase/cellobiase composite enzyme and preparation method thereof

A technology of cellobiase and xylanase, applied in the biological field, can solve the problems of lack of cellobiase, affecting the degradation rate of cellulose, etc., and achieve the effect of solving environmental pollution

Active Publication Date: 2015-06-17
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since cellobiose has a strong inhibitory effect on C1 enzyme and Cx enzyme activity, if the cellobiase component is lacking in the cellulase complex component, the degradation rate of cellulose will be seriously affected
However, currently known cellulase production strains lack cellobiase components, so it is necessary to add exogenous cellobiase in the production process

Method used

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  • Xylanase/cellobiase composite enzyme and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Select the slant of the Aspergillus niger CGMCC3.3147 strain that grows well, gently pick the slant spores and insert them into the bran bottle of the medium containing 20% ​​bran and 70% corn stalks, put a cotton plug on it and wrap it with kraft paper. Shake the glutinous gluten bottle to disperse the spores evenly in the culture medium, move the gluten gluten bottle to the culture room, and cultivate it at 27°C. After culturing to the 10th day, the spores were completely covered with the whole culture medium and then moved into the refrigerator for later use.

[0030] The composition of the solid fermentation medium is 20% bran, 70% corn stalks, 1% urea, 0.2% ammonium sulfate, 0.03% potassium dihydrogen phosphate, 0.002% magnesium sulfate, 0.0001% cobalt chloride, the balance of water, the initial pH5 .6.

[0031] After adding water, stir evenly, and the water content is 65%. Fill the solid medium into a shallow dish and autoclave at 121°C for 60 minutes. Pour ste...

Embodiment 2

[0034] Select the slant of the Aspergillus niger CGMCC3.3147 strain that grows well, gently pick the spores of the slant and insert it into a bran bottle containing 20% ​​bran and 70% wheat straw medium, plug it with a cotton plug and wrap it with kraft paper, shake vigorously Homogenize the bran koji bottle to disperse the spores evenly in the culture medium, move the bran koji bottle to the culture room, and cultivate it at 28°C. After cultivating to the 7th day, the spores were completely covered with the whole culture medium and then moved into the cold room for later use.

[0035] The composition of the solid medium is 20% bran, 70% wheat straw, 1.5% urea, 0.2% ammonium sulfate, 0.04% potassium dihydrogen phosphate, 0.003% magnesium sulfate, 0.0002% cobalt chloride, the balance is water, and the initial pH is 5. 6. After adding water, stir evenly, and the water content is 70%.

[0036] Fill the solid medium into a shallow dish and autoclave at 121°C for 60 minutes. Pou...

Embodiment 3

[0038] Select the slant of the Aspergillus niger CGMCC3.3147 strain that grows well, gently pick the slant spores and insert them into a bran bottle containing 30% bran and 60% straw medium, put a cotton plug on it and wrap it with kraft paper, shake vigorously The bran koji bottle can disperse the spores evenly in the culture medium, and the bran koji bottle is moved to the culture room, and cultured at 27°C. After culturing to the 10th day, the spores were completely covered with the whole culture medium and then moved into the refrigerator for later use.

[0039]The composition of the solid medium is 30% bran, 60% straw, 2.0% urea, 0.1% ammonium sulfate, 0.04% potassium dihydrogen phosphate, 0.003% magnesium sulfate, 0.0005% cobalt chloride, the balance is water, and the initial pH is 6.0 .

[0040] After adding water, stir evenly, and the water content is 75%. Fill the solid medium into a shallow dish and autoclave at 121°C for 60 minutes. Pour sterilized water into the...

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Abstract

The invention provides a xylanase / cellobiase composite enzyme prepared by using Aspergillus niger CGMCC 3.3147 and a preparation method thereof. The xylanase and the cellobiase can be efficiently co-produced through the Aspergillus niger, wherein the produced xylanase can reach 10,000 IU / g and the cellobiase can reach 900 IU / g through a preferable fermentation culture medium; and the prepared composite enzyme can be mixed-used directly with the cellobiase in the production of straw fermented alcohol, xylitol, citric acid and the like. The composite enzyme and the preparation method have great realistic significance for solving the problems of environment pollution, food shortage and energy crisis.

Description

technical field [0001] The invention relates to the biological field, in particular to a xylanase / cellobiase composite enzyme and a preparation method thereof. Background technique [0002] The content of xylan in plant cell walls is second only to cellulose, and is the most abundant hemicellulose. Cellulose, hemicellulose, and lignin account for 30-40%, 15-30%, and 10-20% of the fibrous raw materials, respectively. Among them, xylan is the main component of hemicellulose. Xylan is a polymer composed of D-xylose backbone (linked by β-1,4 bonds) and L-arabinose branches (linked by a-1,2 and a-1,3). It binds to the surface of cellulose microfibrils and interconnects to form a rigid network of interconnected cells. This hard network hinders the adsorption of cellulase to cellulose, thereby affecting the degradation rate of cellulose. At present, the alcoholic fermentation of cellulosic raw materials mainly utilizes the six-carbon sugar obtained by degrading cellulose with c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12P7/10C12P7/18C12P7/48C12R1/685
CPCY02E50/10
Inventor 李荣杰薛培俭穆晓玲李维理
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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