A method for rapidly preparing high-activity cellulase with low-cost carbon source

A cellulase, high activity technology, applied in the biological field, can solve the problems of long fermentation production time and low enzyme activity

Active Publication Date: 2020-12-29
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the traditional process uses a single strain to ferment cellulase, the enzyme activity is low, and the fermentation production time is too long

Method used

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  • A method for rapidly preparing high-activity cellulase with low-cost carbon source
  • A method for rapidly preparing high-activity cellulase with low-cost carbon source

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] use Trichoderma reesei ATCC 56764, Fusarium oxysporum BN and Aspergillus fumigatus HY Production of cellulase by fermentation.

[0019] 1. Source of the strain

[0020] Trichoderma reesei ATCC 56764 was purchased from the American Type Culture Collection. Fusarium oxysporum GenBank accession No. KM979515 of BN), Aspergillus fumigatus HY GenBank Accession No. KF813024).

[0021] 2. Obtaining of Seed Solution

[0022] Obtaining the seed solution: the Trichoderma reesei ATCC 56764, Fusarium oxysporum BN, Aspergillus fumigatus HY were respectively inoculated on PDA medium (composition of medium per liter: 200g potato, 20g glucose, 15g agar, 1000mL distilled water, natural pH), and cultured at 30°C for 3 days. The spores of each bacterial strain were washed separately with physiological saline, and the spore suspensions of each bacterial strain were mixed to obtain a seed solution. In the seed solution, the number of colony-forming units of all strai...

Embodiment 2

[0039] use Trichoderma reesei ATCC 56764, Fusarium oxysporum BN and Aspergillus fumigatus HY Preparation of cellulase by mixed fermentation.

[0040] 1. Obtaining of Seed Solution

[0041] Will Trichoderma reesei ATCC 56764, Fusarium oxysporum BN, Aspergillus fumigatus HY were respectively inoculated on PDA medium (composition of medium per liter: 200g potato, 20g glucose, 15g agar, 1000mL distilled water, natural pH), and cultured at 30°C for 3 days. The spores of each bacterial strain were washed separately with physiological saline, and then mixed to obtain a seed liquid. In the seed solution, the number of colony-forming units of all strains was 1×10 7 CFU / ml), where Trichoderma reesei ATCC 56764, Fusarium oxysporum BN, Aspergillus fumigatus The ratio of the number of HY colony forming units was 15:5:10.

[0042] 2. Preparation of Cellulase

[0043] The fermentation medium contains the following components: licorice residue (water content 5%, cr...

Embodiment 3

[0049] use Trichoderma reesei ATCC 56764, Fusarium oxysporum BN and Aspergillus fumigatus HY Preparation of cellulase by mixed fermentation.

[0050] 1. Obtaining of Seed Solution

[0051] Will Trichoderma reesei ATCC 56764, Fusarium oxysporum BN, Aspergillus fumigatus HY were respectively inoculated on PDA medium (composition of medium per liter: 200g potato, 20g glucose, 15g agar, 1000mL distilled water, natural pH), and cultured at 30°C for 3 days. The spores of each bacterial strain were washed separately with physiological saline, and then mixed to obtain a seed solution. In the seed solution, the number of colony-forming units of all strains was 1×10 7 CFU / ml, where Trichoderma reesei ATCC 56764, Fusarium oxysporum BN, Aspergillus fumigatus The ratio of the number of HY colony forming units was 15:6:15.

[0052] 2. Preparation of Cellulase

[0053] The fermentation medium contains the following components: licorice residue (water content 5%, c...

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Abstract

The invention provides a method for rapidly preparing high activity cellulase by using a cheap carbon source, relating to the technical field of biology. In the method for rapidly preparing high activity cellulase by using the cheap carbon source, liquorice residue is used as the carbon source, Trichoderma reesei, fusarium oxysporum and aspergillus fumigatus are mixed and fermented so as to prepare the high activity cellulase. Trichoderma reesei ATCC 56764, Fusarium oxysporum BN and Aspergillus fumigatus HY are mixed and fermented, thereby remarkably shortening fermentation time and remarkablyimproving the activity of cellulase. The activity of the cellulase generated through mixed fermentation of the three strains is greater than the sum of activities of the cellulase generated by the three strains separately, that is, the three strains play a synergistic role through mixed fermentation.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for rapidly preparing high-activity cellulase with a low-cost carbon source. Background technique [0002] With the development of the economy, human beings have an increasing demand for non-renewable fossil energy such as oil, natural gas, and coal, which has caused many problems such as energy security, oil consumption, and global warming. These problems have prompted countries all over the world to accelerate research on safe, clean and renewable alternative energy sources. Fuel ethanol is a clean alternative energy source that has made great progress in current research and application. However, its production currently mainly relies on starch and sugar raw materials. The use of these two raw materials involves competition with humans and animals for food and land. The resulting rise in food prices has emerged. The development of cheap raw materials other than grain is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12R1/885C12R1/77C12R1/66
CPCC12N9/2437C12Y302/01004
Inventor 许家兴贺爱永夏军吴真胡磊徐宁
Owner HUAIYIN TEACHERS COLLEGE
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