Method for reducing cadmium content of rice grains in cadmium-polluted rice field by using cadmium-resisting microorganisms

A technology for rice grains and microbes, applied in the field of bioremediation of heavy metal-contaminated soil, to achieve good ecological and social benefits, easy operation, and wide application range

Inactive Publication Date: 2015-12-30
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Numerous studies have shown that long-term cadmium pollution can lead to the development of a large number of resistant microbial strains

Method used

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  • Method for reducing cadmium content of rice grains in cadmium-polluted rice field by using cadmium-resisting microorganisms
  • Method for reducing cadmium content of rice grains in cadmium-polluted rice field by using cadmium-resisting microorganisms
  • Method for reducing cadmium content of rice grains in cadmium-polluted rice field by using cadmium-resisting microorganisms

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Experimental program
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Effect test

preparation example Construction

[0079] ③ Preparation of cadmium-resistant microbial culture substrate

[0080] A. Determination of optimum temperature and pH of Aspergillus aculeatus and Bacillus cereus:

[0081] Inoculate 1mL of the bacterial suspension of the two strains into 200mL of newly prepared and sterilized liquid medium, and adjust according to different temperature (10, 15, 20, 25, 30, 35, 40°C) and pH value (4, 5°C). , 6, 7, 8, 9) under the conditions of shaking culture for 36 hours, by measuring the absorbance value, determine the growth temperature of the strain;

[0082] Bacillus cereus ( Bacillus cereus ) grows well at 15-40°C, can grow at pH 4-9, and the optimum pH is 6-7; Aspergillus aculeatus fungus ( Aspergillus aculeatus ) grows well at 10-35°C, can grow at pH 4-7, and the optimum pH is 6;

[0083] B. Preparation of bacterial suspension:

[0084] Take the cadmium-resistant fungi and bacteria obtained from the above screening, and insert them into the corresponding sterile liquid cul...

Embodiment 1

[0103] 1. Embodiment one: adaptability test

[0104] The culture medium was sawdust and sand (v / v=3:1, pH=6.5); the culture medium was divided into two parts, and one part was inoculated with the suspension of the two strains screened above at 5% inoculum (1:1 Proportional mixing), the other was not inoculated. The rice seedlings that had been raised were planted in the two substrates respectively, and the other substrate inoculated with cadmium-tolerant microorganisms was left idle; fertilized with the nutrient solution formula of the International Rice Research Institute, and treated at 7 days, 21 days, 40 days and 60 days respectively The rice rhizosphere soil and idle soil were taken to screen for microorganisms while observing the growth of rice.

[0105] Analysis of the test results: The test results showed that during the whole treatment period, the growth of rice in the matrix of the inoculated strain and the non-inoculated strain were consistent, and the growth cycle...

Embodiment 2

[0106] 2, embodiment two: pot test

[0107] The indica rice '9311' was selected as the material, moved to non-polluted paddy soil for 30 days after germination, and fertilized with the nutrient solution formula of the International Rice Research Institute; v / v=3:1, pH=6.5), mix well and put it in a sterilizing pot (127°C) for high temperature sterilization for 1h, with 1.5mMCdSO 4The solution was poured until the soil cadmium concentration reached 20mg / kg, and after a week of balance, the Aspergillus aculeatus fungus and Bacillus cereus (1:1) were inoculated, treated with cadmium alone, cadmium plus Aspergillus aculeatus fungus, and cadmium plus Bacillus cereus The treatment was the control; the prepared matrix was divided into plastic buckets (40cm in height, 25cm in diameter), the rice seedlings raised were rinsed with tap water, rinsed with sterilized ultrapure water three times, and then planted in the above plastic buckets. Three plants per barrel; 5 groups of treatments...

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Abstract

The invention discloses a method for reducing the cadmium content of rice grains in a cadmium-polluted rice field by using cadmium-resisting microorganisms, belonging to the technical field of biological remediation of heavy metal polluted soil. The method comprises the following steps: (1) screening and purifying the cadmium-resisting microorganisms to obtain two microorganism strains capable of resisting 300mg / L of cadmium; (2) identifying the cadmium-resisting microorganisms, wherein the cadmium-resisting microorganisms are identified to be aspergillus aculeatus and bacillus cereus; (3) preparing a cadmium-resisting microorganism culture substrate; and (4) applying the cadmium-resisting microorganism culture substrate in the rice field. According to the method disclosed by the invention, the screened cadmium-resisting microorganism strains come from rhizosphere soil of dominant plants of a cadmium polluted mine area, and have very good environment adaptability; the effect of the mixed strains of the aspergillus aculeatus and the bacillus cereus is remarkably better than the single application of the aspergillus aculeatus or the bacillus cereus, so that the repairing efficiency is improved; and the cost is low, the operation is simple and convenient and the effect is remarkable. The method is applicable to the biological remediation of the rice field oil with relatively light cadmium pollution.

Description

technical field [0001] The invention belongs to the technical field of bioremediation of heavy metal polluted soil, and in particular relates to a method for reducing the cadmium content of rice grains in cadmium-polluted paddy fields by utilizing cadmium-tolerant microorganisms. Background technique [0002] Cadmium is one of the most toxic heavy metal elements. Cadmium absorbed by plants can affect human health through the food chain. With the development of industry, the aggravation of urban pollution and the increase in the types and quantities of agricultural chemicals, soil cadmium pollution is becoming more and more serious. Rice is one of the three major food crops in my country. When the cadmium content absorbed and accumulated in rice plants exceeds a certain threshold, it will have adverse effects on the growth and development of rice, as well as the yield and quality of rice, and even affect the quality and safety of rice. Under the biomagnification of the food c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G16/00A01G22/22
Inventor 傅金民谢燕娄燕宏
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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