33 results about "Rare organisms" patented technology

The invention relates to a method for constructing a deep pool and a shoal in a straight river channel. In the method, a spur dike is arranged on the left bank side of the straight river channel, a bank slope on the opposite bank of the spur dike is protected by riprap, a diving guide wall is arranged on the left and right banks of the downstream side of the spur dike respectively, the discharge cross section of the river channel is shortened by the spur dike to increase the flow rate of water flow, the bottom of a river bed of a river reach between the diving guide walls on the downstream side of the spur dike is continuously scoured to naturally form the deep pool, and a dead water area or slow flow water area formed between the diving guide wall and the river bank becomes the shoal through long-term deposition. The method has the advantages that: the deep pool is naturally formed and becomes a rest place for aquatic organisms such as fishes and the like, and can become a shelter for the aquatic organisms such as the fishes and the like in a flood period; plants can be planted on the shoal, and the shoal can become an organism habitat different from the deep pool; and the complexity of a river channel structure and water conservation condition diversity can be increased or repaired, and river biodiversity, population composition and rare species conservation are facilitated.

The invention relates to a circular agriculture system and an implementation method thereof. According to a characteristic that different biological wastes can be highly efficiently cycled by various insects and small animals, an insect breeding factory is constructed; and insect and small animal proteasome and insect feces residue organic fertilizer are respectively utilized as an animal factory protein feed and a plant factory organic fertilizer. Therefore, carious animal and plant products with high added values can be produced with high yields. With modern advanced agricultural technologies and equipment, stereo cultivation is usually carried out with rare species, such that a unit yield of the types of factories reaches more than a hundred times that of conventional agriculture. With the products from the three types of factories, a wide variety of series downstream industry chain group can be developed, such that best economic benefit can be ensured. The three types of factories, downstream industry chain spaces and farmer residential spaces can be arranged in a rise building. The buildings can form a highly clustered production, management, residence, agriculture, industry and business integral community. With farmland novel industry and landscape designs outside the buildings, a novel intensive town is formed.

The invention relates to a propagation method of fruit trees, and discloses a propagation method of wufu persimmon seedlings. The method comprises the steps of cleaning and disinfecting wufu persimmonpulp and then preparing the wufu persimmon pulp into fruit leaven; putting wufu persimmon seeds into the fruit leaven to be soaked, and promoting seed germination and rooting; after transplanting, proportioning quantitative clean water for the fruit leaven, watering bud seedlings periodically, using the fruit leaven to provide original nutrients for seedlings, and finally enabling the seedlings to grow into normal and robust plants. In this way, the endangered rare species is rescued. By means of the propagation method of the wufu persimmon seedlings, the survival rate of the wufu persimmon seed-raising seedlings exceeds 80%, the limit of non-propagation of the wufu persimmon seedlings is broken through, and the propagation method is significant in protecting, studying, protecting, applying and popularizing the species.

This invention provides a social network for wildlife species observers, e.g. birders, to verify accuracy of observations made of wildlife in the field, and includes issuance of automatic rare species alerts when a sighting is determined to be rare, all via a web site.

The invention relates to a method for field culture of cordyceps sinensis and vegetation recovery in alpine-cold cordyceps growing areas. Cordyceps sinensis is an insect herb which is formed by hepialus larvae (host) after being infected by fungus cordyceps sinensis and is a special product of a biological chain of fungus, insects and plant in a special habitat. The invention belongs to Field Culture of Wild Medicine biological engineering and has the advantages of proliferating fungus and the hosts in alpine-cold road and field growing areas, increasing and protecting alpine meadow and alpineshrub and meadow vegetation, compensating the activity of the biological chain, developing geographical (biological) resources of cordyceps sinensis habitat which is difficult to simulate by human, decreasing natural enemy hazard to protect the rare species of cordyceps sinensis with stable production and good quality, and protecting and improving the fragile alpine meadow vegetation. The methodadopts different species of hosts for egg-increasing assistance by levels and regions to increase the hatching rate and the larva density; the fungus is separated and purified, and the fungus source is provided through ferment, so that the infection rate of insects by fungus is improved; meanwhile, the favorite plant of host larva is compensated, and growth regulator and special fertilizer are applied to protect the alpine meadow vegetation.

The invention discloses a transplantation method for Michelia guangdongensis. The transplantation method comprises the following steps of (1) digging tree caves, (2) trimming the Michelia guangdongensis, (3) digging seedlings, (4) wrapping the seedlings, (5) transporting the seedlings, (6) fixed-planting the seedlings and (7) performing management after plantation. Growth features of the Michelia guangdongensis are combined with transplantation technology and culturing technology, and the Michelia guangdongensis can be quickly suitable for soil and environment of the transplantation land and quickly root, emergence leaves, restore tree vigor and achieve greening effect; more than 90% of survival rate can be achieved; the Michelia guangdongensis, rare species can be effectively protected; and the transplantation method is advantaged by simple operation and easy promotion and application.

The invention relates to a Sinocyclocheilus anshuiensis spleen cell line construction method. The construction method comprises the following steps: (1) obtaining a Sinocyclocheilus anshuiensis spleen: directly disinfecting a fish body by adopting ethyl alcohol; (2) performing primary culture: culturing by adopting B-15 culture solution containing glutamine, fetal calf serum, penicillin, streptomycin, gentamicin and amphotericin B; (3) subculturing: when culturing to the eighth generation, changing the cell culture solution into basic culture solution, thus successfully constructing a Sinocyclocheilus anshuiensis spleen cell line. The Sinocyclocheilus anshuiensis spleen cell line obtained by the construction method can realize continuous passage, is directly applied to biological characteristic research, and can meet the requirements of germplasm resource conservation and theoretical research on Sinocyclocheilus anshuiensis as a rare species; more importantly, the cell line is the first cell line of cave fish in China, and lays the foundation for the cellular level cave adaptability research.

The invention relates to a library construction method applicable to a cell line and animal tissue ATAC-seq sequencing technology. The method comprises steps as follows: 1) cell or animal tissue is collected, and a cell suspension is prepared; 2) a lysate containing NP40 is added to split cell membranes, and cell nucleuses are obtained and purified; 3) the cell nucleuses are collected for quantitative and integrity detection; 4) Tn5 transposase is added to complete cell nucleuses for a transposition reaction and purification; 5) PCR amplification is performed, and a second library is constructed and purified; 6) high-throughput sequencing is performed, and gene data are obtained. According to the method, quantitative operation is performed directly through the DNA content on the basis of the characteristic that transposase is sensitive to DNA content, and the method is wide in applicability and particularly applicable to library construction of rare species or tissue and tissue in poorpreservation state. Meanwhile, the recycled cell nucleuses can be used for Tn5 transposase developed by domestic vazyme company through early optimization, good experiment results are obtained, and ATAC-seq is not limited by the variety of transposase any more.

The invention provides a nicotine-decomposing microorganism selective medium and a preparation method thereof and relates to the technical field of microorganism cultivation. The nicotine-decomposing microorganism selective medium and the preparation method thereof give consideration to wide-adaptability and pertinence of formulas, improve the diversity of nicotine-decomposing microorganism selective sources, reduce unnecessary repeated selection, improve microorganism isolation rate of nicotine-decomposing microorganism rare species and enrich the demand resources of high-efficiency nicotine-decomposing microorganisms in the practical production and research field. The selective medium comprises 2g of beef extract, 1g of yeast powder, 3g of gellan gum, 0.5g of trehalose, 13g of K2HPO4, 4g of KH2PO4, 0.5g of (NK4)2SO4, 5g of NaCl, 0.5g of CaCl, 600 ml of sample environmental soil extract, 1g of nicotine, 18g of agar powder, wherein distilled water is added until the volume reaches1000 ml and natural pH is reached.

The invention relates to a rare species DNA information acquiring method, and belongs to the technical field of measuring or detecting methods containing enzyme or microorganisms. The method comprisesthe following steps: after acquiring a tissue of a south China tiger, purifying DNA, and enabling the DNA to generate random fragmentation, separating the DNA, and recovering, performing a A-adding catalytic reaction on the recovered DNA, and performing a coupled reaction on a T-carrier, performing conversion on a coupled product, screening a positive bacterial colony, culturing overnight, performing plasmid recovery, sequencing, and performing a specific primer design, synthetizing, and implementing PCR again and sequencing a PCR product, performing comparison with a sequence used in the primer design, if a result is consistent, it is demonstrated that the DNA fragment is the DNA of the target species surely. The method is used for acquiring DNA information of the rare species, and has the advantages of simple and convenient operation, high acquiring rate, low cost and the like.

The invention provides a new strain of Agaricus bernadii Mengbaiyin No.2. The new strain of Agaricus bernadii Mengbaiyin No.2 is cultivated from wild Agaricus bernadii which is commonly known as Agaricus arvensis, and has a preservation number of CGMCC No.15086. The invention also provides a method for preparing and cultivating a strain of the wild Agaricus arvensis. The method comprises preparation of the strain, propagation, preparation of original seeds and preparation and cultivation of cultivated seeds. Not only can the endangered rare species of Agaricus arvensis be protected so as to develop a new economic growth point besides grass and livestock for an pastoral area, but also manure of cattle and sheep and grass in the pastoral area can be turned into treasure; in summer, local herders can use idle cattle and sheep pens to plant the Mengbaiyin No.2 to increase income, so that a new way of an ecological economy in the pastoral area is developed.

The invention discloses river or lake rare germplasm resource migration and protection equipment and a using method thereof. The river or lake rare germplasm resource migration and protection equipment comprises a boat and a control room, wherein the control room is arranged on the boat; an organism temporary storage box and a power system are arranged on the boat; the organism temporary storage box can be placed in a river or lake, and lifting of the organism temporary storage box is controlled by the power system; the organism temporary storage box is a box of which the top is not closed, but the periphery is closed and a bottom plate is movable; the movable bottom plate consists of two butt plates which are controlled to be opened or closed by a hydraulic cylinder; and a watertight sealis arranged at a position where the two butt plates are combined. Rare germplasm resources in the river or lake are protected when the river or lake is dredged. According to the provided structure, the organism temporary storage box is arranged on the boat, before dredging through the equipment, rare organisms in the river or lake are migrated from original positions, and then dredging construction of bottom sediment of the river or lake is carried out; and after dredging is finished, the rare organisms are placed back to the original positions. While the river or lake is dredged, the rare germplasm resources in the lake are protected, diversity of species of the river or lake is maintained favorably, and thus, the equipment has great significance on protection of the germplasm resourcesin the lake.

The invention discloses efficient taxol-producing endophytic fungi aspergillus aculeatus Tax-6 and application thereof. The efficient taxol-producing endophytic fungi has the classification and name of Aspergillus aculeatus Tax-6 and is preserved in China Center for Type Culture Collection with the preservation number of CCTCC M 2016614, the preservation date of November 3rd, 2016 and the preservation address of Wuhan University in Wuhan, China. According to the invention, Tax-6 is cultivated by virtue of a liquid fermentation technology, then taxol is extracted from fermentation broth, the highest content reaches 334.92mu g/L, and cultivation time is only 7 days. The endophytic fungi on the bark of taxus chinensis are utilized for carrying out fermentation and producing taxol, an efficient anti-cancer drug taxol is obtained with low cost, and the rare species taxus chinensis is protected.

The invention relates to a method for constructing a sinocyclocheilus rhinocerous heart cell line. The method comprises the following steps: 1, obtaining of sinocyclocheilus rhinocerous heart tissues, wherein sinocyclocheilus rhinocerous is sterilized through combination of penicillin and ethyl alcohol; 2, primary culture, wherein a culture solution M199 which contains glutamine, penicillin, streptomycin, streptomycin sulphate and amphotericin is adopted for culture, and the osmotic pressure of the culture solution is 280-330 mOsm/l; 3, secondary culture, wherein when the tenth generation is reached, cell culture fluid is replaced with base culture fluid, and the sinocyclocheilus rhinocerous heart cell line is constructed successfully. According to the method for constructing the sinocyclocheilus rhinocerous heart cell line, the obtained sinocyclocheilus rhinocerous heart cell line is in the fibrous form and can conduct continuous passage and be directly applied to research of biological characteristics, requirements of genetic resource preservation and theoretical research conducted on the rare species, namely the sinocyclocheilus rhinocerous, can be satisfied, meanwhile, the heart cell line is the first heart cell line of cave fishes in China, and the foundation is laid for cave adaptability research at the cellular level.