Nicotine-decomposing microorganism selective medium and preparation method thereof

A technology for screening culture medium and nicotine-degrading bacteria, which is applied in the direction of bacteria, adding compounds to stimulate growth, etc., can solve the problems of unsuitable screening, difficulty in screening out rare bacterial resources, and poor diversity of nicotine-degrading bacteria.

Inactive Publication Date: 2015-03-04
LUOHE COMPANY OF HENAN TOBACCO
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Although the above medium formula can be used to screen nicotine-degrading bacteria, the existing problems are also more prominent. These shortcomings are concentrated in: (1) not suitable for the screening of nicotine-degrading bacteria in special types of soil samples; (2) trace element imbalance , the objectivity is not strong; (3) The diversity of the screened nicotine-degrading bacteria is poor, and it is difficult to screen out rare bacteria resources

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  • Nicotine-decomposing microorganism selective medium and preparation method thereof
  • Nicotine-decomposing microorganism selective medium and preparation method thereof
  • Nicotine-decomposing microorganism selective medium and preparation method thereof

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Embodiment Construction

[0018] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0019] The embodiment of the present invention provides a screening medium for nicotine-degrading bacteria, comprising: 2 g of beef extract, 1 g of yeast powder, 3 g of gellan gum, 0.5 g of trehalose, K 2 HPO 4 13g, KH 2 PO 4g, (NH 4 ) 2 SO 4 0.5g, NaCl 5g, CaCl 2 0.5g, sample environment soil extract 600ml, nicotine 1g, agar powder 18g, distilled water to 1000ml, natural pH.

[0020] Optionally, the formula of the nicotine-degrading bacteria screenin...

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Abstract

The invention provides a nicotine-decomposing microorganism selective medium and a preparation method thereof and relates to the technical field of microorganism cultivation. The nicotine-decomposing microorganism selective medium and the preparation method thereof give consideration to wide-adaptability and pertinence of formulas, improve the diversity of nicotine-decomposing microorganism selective sources, reduce unnecessary repeated selection, improve microorganism isolation rate of nicotine-decomposing microorganism rare species and enrich the demand resources of high-efficiency nicotine-decomposing microorganisms in the practical production and research field. The selective medium comprises 2g of beef extract, 1g of yeast powder, 3g of gellan gum, 0.5g of trehalose, 13g of K2HPO4, 4g of KH2PO4, 0.5g of (NK4)2SO4, 5g of NaCl, 0.5g of CaCl, 600 ml of sample environmental soil extract, 1g of nicotine, 18g of agar powder, wherein distilled water is added until the volume reaches1000 ml and natural pH is reached.

Description

technical field [0001] The invention relates to the technical field of microorganism culture, in particular to a screening medium for nicotine-degrading bacteria and a preparation method thereof. Background technique [0002] Nicotine, commonly known as nicotine, is an alkaloid present in Solanaceae plants (Solanum genus), and is also an important component of tobacco, accounting for more than 95% of tobacco alkaloids. Long-term excessive intake of nicotine by the human body will paralyze the heart, inhibit the central nervous system, and greatly increase the chances of suffering from respiratory system, cardiovascular and cerebrovascular diseases. The N-nitrosammonium produced by the nitrosation of nicotine during the combustion of flue-cured tobacco is a strong carcinogen, which has great health hazards to both active and passive smokers. Therefore, how to control and reduce the nicotine content in tobacco leaves is a thorny problem faced by both tobacco planting and ciga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/20
CPCC12N1/20
Inventor 朱金峰孙会忠王小东李广良孙明辉陈冲
Owner LUOHE COMPANY OF HENAN TOBACCO
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