Breeding method for tissue culture seedlings of Guangxi wild Adinandra nitida

A technique for wild stone cliff tea and formula, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of low reproduction efficiency and inapplicability of cutting technology, and achieve the effects of simple operation and high survival rate.

Active Publication Date: 2018-04-13
广西金秀瑶族自治县君临茶业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The propagation efficiency of cutting technology is low, and the number and maturity of manual operation technology are required to improve the survival rate of reproduction. It is suitable for farmers to operate by themselves, and is not suitable for factories or large-scale breeding.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for tissue culture and breeding of Guangxi wild Shiya tea, comprising the steps of:

[0021] (1) Picking of explants: From May to June every year, select wild Shiya tea that is above 800 meters above sea level, grows on cliffs all year round, and has no pests and diseases, and picks its buds.

[0022] (2) Disinfection: Rinse the shoots repeatedly with pure water for 3 to 4 times, disinfect with mercuric chloride solution for 10 to 20 minutes, blot dry with sterilized filter paper, and set aside.

[0023] (3) Callus culture: Carry out the following operations on the ultra-clean workbench: Use a sterilized scalpel to cross-cut the shoots with a length of 1.2-1.5 cm, put the shoots into the callus culture medium and culture them in the The temperature is 28~30℃, the humidity is controlled at 60~85%, and the callus is obtained by dark culture for 15 days; the formula of the callus culture medium is MS+N6 benzyl adenine 0.3mg / L+naphthaleneacetic acid 0.8mg / L+vitamin...

Embodiment 2

[0028] A method for tissue culture and breeding of Guangxi wild Shiya tea, comprising the steps of:

[0029] (1) Picking of explants: From May to June every year, select wild Shiya tea that is above 800 meters above sea level, grows on cliffs all year round, and has no pests and diseases, and picks its buds.

[0030] (2) Disinfection: Rinse the shoots repeatedly with pure water for 3 to 4 times, disinfect with mercuric chloride solution for 10 to 20 minutes, blot dry with sterilized filter paper, and set aside.

[0031] (3) Callus culture: Carry out the following operations on the ultra-clean workbench: Use a sterilized scalpel to cross-cut the shoots with a length of 1.2-1.5 cm, put the shoots into the callus culture medium and culture them in the The temperature is 28~30℃, the humidity is controlled at 60~85%, and the callus is obtained by dark culture for 18 days; the formula of the callus culture medium is MS+N6 benzyl adenine 0.5mg / L+naphthaleneacetic acid 1.5mg / L+vitamin...

Embodiment 3

[0036] A method for tissue culture and breeding of Guangxi wild Shiya tea, comprising the steps of:

[0037] (1) Picking of explants: From May to June every year, select wild Shiya tea that is above 800 meters above sea level, grows on cliffs all year round, and has no pests and diseases, and picks its buds.

[0038] (2) Disinfection: Rinse the shoots repeatedly with pure water for 3 to 4 times, disinfect with mercuric chloride solution for 10 to 20 minutes, blot dry with sterilized filter paper, and set aside.

[0039] (3) Callus culture: Carry out the following operations on the ultra-clean workbench: Use a sterilized scalpel to cross-cut the shoots with a length of 1.2-1.5 cm, put the shoots into the callus culture medium and culture them in the The temperature is 28~30℃, the humidity is controlled at 60~85%, and the callus is obtained by dark culture for 168 days; the formula of the callus culture medium is MS+N6 benzyl adenine 0.4mg / L+naphthaleneacetic acid 0.8mg / L+vitamin ...

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PUM

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Abstract

The invention discloses a breeding method for tissue culture seedlings of Guangxi wild Adinandra nitida. The breeding method comprises the following steps: plucking of explant; disinfection; callus culture; culture of aseptic sprouts of Adinandra nitida; and rooting culture of the aseptic sprouts of Adinandra nitida. The breeding method provided by the invention is simple to operate, high in survival rate, capable of maintaining the excellent properties of wild Adinandra nitida and applicable to industrial breeding of the tissue culture seedlings; and the method is of critical significance andimmeasurable commercial value to promotion of the rare species, i.e., wild Adinandra nitida.

Description

technical field [0001] The invention belongs to the field of crop breeding, and in particular relates to a method for breeding wild Shiya tea tissue culture seedlings in Guangxi. Background technique [0002] Shiya tea, the scientific name is Bright Ye Huang Ruimu, Yangtong is a tree. It is mainly grown in the southern mountainous area of ​​Jinxiu Yao Autonomous County, Guangxi Zhuang Autonomous Region. It likes short sunshine, large temperature difference, cool and humid rocky cliffs or mountain streams, and because the tea grows on cliffs, it can be said to absorb the aura of heaven and earth, and the essence of the sun and the moon; The unique ecological environment has bred a unique style of pure natural drinks. In ancient times, monkeys were trained to pick tea, which is also called "monkey picking tea" among the people. Tested by relevant departments, because Shiya tea does not contain caffeine, it does not affect sleep after drinking, and it is rich in flavonoids, wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 梁干君钟春城李秋玲
Owner 广西金秀瑶族自治县君临茶业有限公司
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