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New strain of Agaricus bernadii Mengbaiyin No.2 and cultivation method of new strain of Agaricus bernadii Mengbaiyin No.2

A technology of white-scale mushrooms and new strains, applied in mushroom cultivation, botanical equipment and methods, and medical raw materials derived from fungi, etc., can solve problems such as domestication technology of black mushrooms in wild grasslands that have not yet been seen, and achieve the effect of increasing income

Inactive Publication Date: 2018-07-13
INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on domestication technology of black mushroom in wild grassland

Method used

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  • New strain of Agaricus bernadii Mengbaiyin No.2 and cultivation method of new strain of Agaricus bernadii Mengbaiyin No.2
  • New strain of Agaricus bernadii Mengbaiyin No.2 and cultivation method of new strain of Agaricus bernadii Mengbaiyin No.2
  • New strain of Agaricus bernadii Mengbaiyin No.2 and cultivation method of new strain of Agaricus bernadii Mengbaiyin No.2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 bacterial classification makes

[0043] 1) Production of mother species: collect strong and wild prairie black mushrooms to make mother species, cut open fresh wild prairie black mushroom fruiting bodies under aseptic conditions, select the fungus meat tissue at the junction of the cap and stipe, and transfer it into a PDA-improved Put it into a constant temperature incubator in a medium container and cultivate it at a temperature of 25°C to 28°C for 20 to 30 days until the mycelium is overgrown.

[0044] 2) Propagation: the mother species is transferred to the improved PDA medium, and cultivated at 25°C-28°C for 20-25 days until the mycelium is covered.

[0045] The PDA improved medium is: containing sweet potato 200g / L, sucrose 10g / L, glucose 10g / L, yeast powder 1.5g / L, peptone 1.5g / L, magnesium sulfate 0.5g / L, dipotassium hydrogen phosphate 1g / L, Agar 10g / L culture medium. Sterilization conditions: 121°C ~ 125°C (pressure 1.1kg / cm 2 ~1.5kg / cm 2 ) to ...

Embodiment 2

[0050] Embodiment 2 bacterial classification makes

[0051] 1) Production of mother species: collect strong and wild prairie black mushrooms to make mother species, and use iron wire to pass through the fruiting bodies of fresh wild prairie black mushrooms under sterile conditions, so that the gills hang downwards in jars to collect basidiospores , pick a few basidiospores into a test tube, add sterile water to dilute to 100 μL containing 40 to 60 basidiospores, and obtain a basidiospore suspension. Draw the basidiospore suspension and add dropwise to the PDA improved medium (only the carbon source in the PDA improved medium formula of Example 1 is changed to bran 100 / L), and spread evenly, at 25°C~28°C Cultivate at a certain temperature for 20-40 days until the mycelium is overgrown.

[0052] 2) Propagation: the mother species is transferred to the improved PDA medium, and cultured at 25° C. to 28° C. for 20 to 25 days until the hyphae are covered.

[0053] PDA improved liq...

Embodiment 3

[0057] Embodiment 3 bacterial classification makes

[0058] 1) Production of mother species: collect strong and wild prairie black mushrooms to make mother species, cut open fresh wild prairie black mushroom fruiting bodies under aseptic conditions, select the fungus meat tissue at the junction of the cap and stipe, and transfer it into a PDA-improved Culture medium (only change the carbon source in the PDA improved medium formula of embodiment 1 into potato 200g / L), put it into a constant temperature incubator and cultivate it, and cultivate it for 20~30 days at a temperature of 25°C~28°C , until the mycelium is overgrown.

[0059] 2) Propagation: Transfer the mother species to the improved PDA liquid medium, and culture at 25°C to 28°C at a speed of 130r / min for 7 to 10 days. The cultured bacteria balls evenly cover the vessel to complete the production of liquid strains.

[0060] PDA improved medium: containing 200g / L potato, 10g / L sucrose, 10g / L glucose, 1.5g / L yeast powd...

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Abstract

The invention provides a new strain of Agaricus bernadii Mengbaiyin No.2. The new strain of Agaricus bernadii Mengbaiyin No.2 is cultivated from wild Agaricus bernadii which is commonly known as Agaricus arvensis, and has a preservation number of CGMCC No.15086. The invention also provides a method for preparing and cultivating a strain of the wild Agaricus arvensis. The method comprises preparation of the strain, propagation, preparation of original seeds and preparation and cultivation of cultivated seeds. Not only can the endangered rare species of Agaricus arvensis be protected so as to develop a new economic growth point besides grass and livestock for an pastoral area, but also manure of cattle and sheep and grass in the pastoral area can be turned into treasure; in summer, local herders can use idle cattle and sheep pens to plant the Mengbaiyin No.2 to increase income, so that a new way of an ecological economy in the pastoral area is developed.

Description

technical field [0001] The invention belongs to the technical field of strain domestication, and in particular relates to a new strain of white scale mushroom No. 2 Mengbaiyin and a domestication method thereof. Background technique [0002] Grassland black mushroom, scientific name white scale mushroom (Agaricus bernardii Quél.). It belongs to the order Agaricales, the family Agaricaceae, and the genus Agaricus. It is mainly distributed in Inner Mongolia, Hebei and other regions. In Inner Mongolia, it is mainly distributed on the grasslands of Xilin Gol and Horqin. The caps of grassland black mushrooms are white or light yellowish brown, with massive polygonal scales. [0003] The scales in the middle of the cap are thicker, fissured, and often rolled back. The scales gradually become thinner and larger from the center of the cap to the edge, and the scales on the edge are often longitudinally cracked. The fungus flesh is thicker and hard white, and the wound turns brown...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/40A23L31/00A23L33/00A23K10/30A61K36/06
CPCA23K10/30A23L31/00A23L33/00A23V2002/00A61K36/06A23V2200/30Y02P60/87
Inventor 孙国琴郭九峰王勇王润元于传宗王海燕边淑萍庞杰李亚娇金焕林
Owner INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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