Tuber Magnatum mycorrhiza synthetic method

Abstract

The invention discloses a Tuber Magnatum mycorrhiza synthetic method and particularly relates to a mycorrhiza synthetic method of domestic Tuber Magnatum (T.panzhihuanense sensu lato) and Pinus armandi. The synthetic method comprises the steps of screening of Pinus armandi seeds, Tuber Magnatum strain and sporocarp thereof, culture of sterile sapling, microbial inoculum preparation, seedling culture matrix preparation, matrix PH value adjustment, matrix screening, microbial inoculum concentration and inoculation requirement, mycorrhiza seedling culture, mycorrhiza morphological anatomy, gene level DNA (deoxyribonucleic acid) molecular detection and confirmation, transplant, cultivation and the like, and provides a necessary key core technology for artificial cultivation of the domestic Tuber Magnatum (T.panzhihuanense sensu lato). The method can be applied to repair and improvement of soil of barren mountain and hillside, farmland-to-forest afforestation and repair and rebuilding of vegetation, has giant potential on developing and utilizing limestone mountain clayey soil regions and remote mountain forest zones, and finally realizes artificial mycorrhiza synthesis, Tuber Magnatum cultivation and the protection and sustainable utilization of rare species. The method is simple to operate and can be widely applied and generalized.

Description

Technical field:

[0001] The invention belongs to the interdisciplinary field of plant and microorganism technology, and in particular relates to a method for synthesizing white truffle mycorrhiza, in particular to a method for synthesizing domestic white truffle and Huashan pine mycorrhiza. Background technique:

[0002] Truffles are called "truffles" in international trade; black ones are called black truffles (black truffles); light-colored ones are called white truffles (white truffles). White truffle is a rare biological resource in nature, which has great scientific research value and irreplaceable ecological value. Because of its special flavor and its price is as high as a diamond, it is known as "the diamond on the tip of the tongue" and "the food of God", and it has become a well-known rare delicacy, and it is the most expensive group in the truffle family. Over the past 10 years, strong international demand and rising prices have led to predatory collection, a sha...