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Tuber Magnatum mycorrhiza synthetic method

A synthesis method and technology of truffles, which are applied in the field of intersection of plant and microorganism technology, and can solve the problems such as no method for synthesizing mycorrhizae of truffles.

Inactive Publication Date: 2014-02-05
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there are no reports and records on the synthetic method of white truffle mycorrhiza and the synthetic method of white truffle and Huashan pine mycorrhizal in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Synthesis of white truffle and pine mycorrhizal seedlings:

[0039] 1. Huashan pine seedling cultivation:

[0040] (1) Preparing species

[0041] Collect mature Huashan pine seeds in autumn and store them in the refrigerator at 4-8°C. The storage period does not exceed 1 year. Take out before use, 30%H 2 o 2Soak for 2 hours, stir several times during the process→wash several times with sterile water→discard the floating seeds→soak in sterile water for 48 hours, remove→use mechanical means to slightly break the seeds→reuse 75% alcohol cotton gauze to wipe and disinfect , put in a petri dish for later use.

[0042] (2) Matrix

[0043] Mix vermiculite (not sieved) and perlite at a volume ratio of 1 / 1, add tap water to make the water content reach 30%, and sterilize with high-pressure steam at 121-126°C for 1 hour; if it is newly produced vermiculite and perlite It can also be used directly without sterilization.

[0044] (3) Sowing

[0045] Sow the prepared seeds i...

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PUM

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Abstract

The invention discloses a Tuber Magnatum mycorrhiza synthetic method and particularly relates to a mycorrhiza synthetic method of domestic Tuber Magnatum (T.panzhihuanense sensu lato) and Pinus armandi. The synthetic method comprises the steps of screening of Pinus armandi seeds, Tuber Magnatum strain and sporocarp thereof, culture of sterile sapling, microbial inoculum preparation, seedling culture matrix preparation, matrix PH value adjustment, matrix screening, microbial inoculum concentration and inoculation requirement, mycorrhiza seedling culture, mycorrhiza morphological anatomy, gene level DNA (deoxyribonucleic acid) molecular detection and confirmation, transplant, cultivation and the like, and provides a necessary key core technology for artificial cultivation of the domestic Tuber Magnatum (T.panzhihuanense sensu lato). The method can be applied to repair and improvement of soil of barren mountain and hillside, farmland-to-forest afforestation and repair and rebuilding of vegetation, has giant potential on developing and utilizing limestone mountain clayey soil regions and remote mountain forest zones, and finally realizes artificial mycorrhiza synthesis, Tuber Magnatum cultivation and the protection and sustainable utilization of rare species. The method is simple to operate and can be widely applied and generalized.

Description

Technical field: [0001] The invention belongs to the interdisciplinary field of plant and microorganism technology, and in particular relates to a method for synthesizing white truffle mycorrhiza, in particular to a method for synthesizing domestic white truffle and Huashan pine mycorrhiza. Background technique: [0002] Truffles are called "truffles" in international trade; black ones are called black truffles (black truffles); light-colored ones are called white truffles (white truffles). White truffle is a rare biological resource in nature, which has great scientific research value and irreplaceable ecological value. Because of its special flavor and its price is as high as a diamond, it is known as "the diamond on the tip of the tongue" and "the food of God", and it has become a well-known rare delicacy, and it is the most expensive group in the truffle family. Over the past 10 years, strong international demand and rising prices have led to predatory collection, a sha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 刘培贵王向华于富强万山平邓晓娟乔鹏赵文青
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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