Breeding method for tissue culture seedlings of excentrodendron hsienmu

A technique for tissue culture seedlings and clams, applied in the field of plant breeding, can solve the problems of rare clams, seedlings and large-scale afforestation, difficulty in seed collection, difficulty in seed collection, etc. high rate effect

Inactive Publication Date: 2018-04-27
DAXIN SCI & TECH INFORMATION RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the extremely difficult seed collection of clam wood, coupled with its low pollination rate and serious fruit drop due to its firmness and reproductive habits, it is difficult to collect seeds.

Method used

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Examples

Experimental program
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Embodiment 1

[0023] Embodiment 1 A kind of propagation method of clam wood tissue culture seedling

[0024] The breeding method of clam wood tissue culture seedling: comprise the steps:

[0025] (1) Harvesting of explants: Select healthy, semi-lignified twigs with 3-7 axillary buds that are free from pests and diseases, remove the leaves, and cut them into 1-2.5cm sections with sterilized scissors, with each section containing at least An axillary bud was used as an explant.

[0026] (2) Disinfection: After repeatedly washing the explants with pure water for 3 to 4 times, first disinfect with sodium hypochlorite solution for 5 to 10 seconds, then disinfect with mercuric chloride solution for 10 to 20 minutes, and dry them with sterilized filter paper. spare.

[0027] (3) Pre-cultivation: The explants were cultured obliquely in the callus culture medium at a temperature of 23-25°C and humidity controlled at 70-80%, and cultured in the dark for 10-15 days to obtain callus.

[0028] (4) Pr...

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Abstract

The invention discloses a breeding method for tissue culture seedlings of excentrodendron hsienmu. The method includes the steps of selecting explants having excellent characters, performing primary culture, subculture and rooting culture, and finally performing domestication and transplanting. The tissue culture seedlings have high survival rate and can maintain the excellent characters well. Byculturing the tissue culture seedlings, excellent characters of the excentrodendron hsienmu can be maintained well, thus greatly increasing the survival rate of the excentrodendron hsienmu. The methodcan solve problems that there is no mature breeding technology of the excentrodendron hsienmu and the excentrodendron hsienmu is difficult to popularize to a large range in the prior art. Through thebreeding method for tissue culture seedlings, survival rate can reach more than 95% and transplanting survival rate after outplanting is more than 85%. The method has great value of continuing the rare species resources.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a breeding method for tissue cultured seedlings of clam tree. Background technique [0002] Clam wood, Latin scientific name: Excentrodendron hsienmu (Chun et How) H. T. Chang et R.H. Miau, Tiliaceae, clamwood is an evergreen large tree, endangered species. National secondary protected plants. Distributed in the monsoon forests of tropical limestone mountains at an altitude of 700 to 900 meters in Guangxi and Yunnan, China. The distribution center of this species is in the south of Guangxi, extending westward to the limestone area in southeastern Yunnan, and crossing the Tropic of Cancer to the north, it gradually becomes an occasional species and disappears. In Yuanjiang, it is the northern edge of the distribution of this species, and it is occasionally seen. Young trees are shade-tolerant, and need full sun for more than 10 years old. It blooms in March...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李贵林
Owner DAXIN SCI & TECH INFORMATION RES INST
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