A method for constructing a rhino-horned catfish heart cell line

A rhinoceros horn catfish and construction method technology, applied in artificial cell constructs, microbial-based methods, animal cells, etc., can solve problems such as no similar reports, achieve repeatability, low pollution rate, and simple and easy operation line effect
CN105349484BActive Publication Date: 2019-03-08KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
Publication Date
2019-03-08

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Abstract

The invention relates to a method for constructing a sinocyclocheilus rhinocerous heart cell line. The method comprises the following steps: 1, obtaining of sinocyclocheilus rhinocerous heart tissues, wherein sinocyclocheilus rhinocerous is sterilized through combination of penicillin and ethyl alcohol; 2, primary culture, wherein a culture solution M199 which contains glutamine, penicillin, streptomycin, streptomycin sulphate and amphotericin is adopted for culture, and the osmotic pressure of the culture solution is 280-330 mOsm / l; 3, secondary culture, wherein when the tenth generation is reached, cell culture fluid is replaced with base culture fluid, and the sinocyclocheilus rhinocerous heart cell line is constructed successfully. According to the method for constructing the sinocyclocheilus rhinocerous heart cell line, the obtained sinocyclocheilus rhinocerous heart cell line is in the fibrous form and can conduct continuous passage and be directly applied to research of biological characteristics, requirements of genetic resource preservation and theoretical research conducted on the rare species, namely the sinocyclocheilus rhinocerous, can be satisfied, meanwhile, the heart cell line is the first heart cell line of cave fishes in China, and the foundation is laid for cave adaptability research at the cellular level.
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Description

technical field

[0001] The invention relates to a method for establishing a cell line by using the heart tissue of the rhino horned barb, and belongs to the technical field of freshwater aquatic organism cell culture and ultra-low temperature cryopreservation. Background technique

[0002] Sinocyclocheilus rhinocerous (Regan, 1904) belongs to the genus Sinocyclocheilus of Cypriniformes (Cypriniformes) Cyprinidae. It is a new cave species published in 1994. At present, the known distribution of this species is The point is the underground river in Huancheng Township, Luoping County, Yunnan Province, which belongs to the Nanpanjiang River system. The research on it is only found in the description of its taxonomic features, the confirmation of its taxonomic status and its phylogenetic relationship.

[0003] Due to the small population of rhino-horned barb and the difficulty of obtaining it, how to protect and preserve the germplasm resources of this rare cave fish has become a...

Claims

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