32results about How to "Short method cycle" patented technology

The invention relates to the field of genetic resource conservation, particularly relates to the field of animal paternity identification, and discloses a method of establishing an animal paternity identification system on basis of whole genome STR. The method comprises the following steps: 1) looking up whole genome STR loci; 2) preliminarily screening the STR loci; 3) carrying out a primer design; 4) randomly sampling animal populations and extracting genome DNA; 5) verifying the population diversity of the STR loci; 6) carrying out Hardy-Weinberg equilibrium inspection on the STR loci with genetic polymorphism, and screening out the STR loci with the genotype frequency distribution which meets the Hardy-Weinberg equilibrium; 7) evaluating the forensic medicine application value of the STR loci; 8) establishing the STP animal paternity identification system. The method is convenient to operate; the constructed paternity identification system has a paternity exclusion probability of more than 0.99, and is capable of accurately carrying out animal paternity identification, therefore, the paternity identification system can be used for guiding the breeding of rare species, and the identification and breeding of economic species.

The invention relates to a preparation method of super-hydrophobic-hydrophilic wood with intelligent temperature response and belongs to the field of super-hydrophobic wood. The super-hydrophobic wood prepared by the method has the hydrophilic reversible characteristic which is not owned by the previous super-hydrophilic wood. A contact angle between the super-hydrophobic wood and water is greater than 150 degrees; a rolling angle is smaller than 10 degrees; the super-hydrophobic-hydrophilic wood is excellent in super-hydrophobic performance and good in self-cleaning performance. The preparation method of the super-hydrophobic-hydrophilic wood with the intelligent temperature response comprises the following steps: soaking the wood in a silicon dioxide aqueous solution, drying, then uniformly and dropwise coating the surface of the wood with polycaprolactone dissolved in a mixed solution of methyl alcohol and chloroform, and finally drying in vacuum. The wood shows the intelligent temperature response in the cross section. The method is simple in process, low in cost, short in period, low in energy consumption and free of special complicated equipment; the prepared super-hydrophobic-hydrophilic wood with the intelligent temperature response is capable of converting the wettability for hundreds of times under high and low temperatures; the prepared super-hydrophobic-hydrophilic wood has a great application prospect in the future.

The invention discloses a preparation method of a PVDF/silicon dioxide aerogel membrane material, and belongs to the field of nano thermal insulation materials. According to the method, PVDF and silicon dioxide aerogel are prepared into a spinning precursor solution, a PVDF fiber/silicon dioxide aerogel non-woven membrane material is prepared through two sets of needle heads by adopting the electrostatic spinning technology, then the PVDF/silicon dioxide aerogel membrane material is formed through hot pressing, and the prepared PVDF/silicon dioxide aerogel membrane material is good in flexibility and free of powder falling.

The invention discloses a tissue culture method of vaccinium dunalianum containing caffeoyl arbutin, relating to the technical field of tissue culture of plants. A tissue culture seedling culture system is built and the content of caffeoyl arbutin is measured by using improved WPM (woody plant medium) as basal medium at different proportion concentrations of hormone and activated carbon. The growth and root induction of aseptic seedling can be controlled by adjusting the proportion of 6-BA, NAA and the activated carbon. The induction rate of explants reaches 97 percent, the bud seedling is robust, the growth is rapid, and the rooting percentage of the bud seedling is 94%. The method has the advantages of rapid reproduction speed, short period and high content of caffeoyl arbutin, and the like, is an effective way to obtain a large number of caffeoyl arbutin, and provides reliable technical basis for large-scale production of fine rapid propagation seedlings of vaccinium dunalianum.

The invention discloses a Zhugen ginger detoxification tissue culture seedling industrialized breeding method, which comprises four steps of induction of shoot-tip clustered shoots, subculture multiplication of the clustered shoots, rooting culture and transplantation of test-tube seedlings and respectively optimizes an induction medium, a multiplication medium, a rooting medium, a transplantation medium and the like. The method has high shoot-tip clustered shoot induction rate, high multiple of subculture multiplication of the clustered shoots and low vitrification ratio; the seedling rooting rate reaches 100 percent; the root system is robust and lateral buds sprout; the transplanting survival rate is high and the seedlings are robust and grow quickly; and the method of the invention has short period and low cost, can completely meet the requirements of large-area scale culture of Zhugen ginger and has good application prospect.

The invention discloses and provides a non-dependent production method of gamma-polyglutamic acid from glutamic acid. The gamma-polyglutamic acid produced by the prior art is low in concentration and high in cost. According to the non-dependent production method disclosed by the invention, an organic acid or a mixture of the organic acid and a nitrogen source is added into a culture medium by flowing during the process of culturing aerobic bacillus sp. to induce thalli to transform a saccharine material and the organic acid into the gamma-polyglutamic acid, and a precursor L-glutamic acid does not need to be additionally added into the culture medium. The fermentation method for producing the gamma-polyglutamic acid, disclosed by the invention, is short in period and easy to operate, can be used for industrial large-scale fermentation and has great application value.

The invention discloses a method of measuring ultrasonic wave transmission time by using chirp signals. The method comprises the following steps: an excitation signal is generated by a chirp signal generator to excite a transmitting transducer to transmit ultrasonic wave, after passing through a material to be measured, a transmitted ultrasonic wave signal is received by a receiving transducer, then wavelet filtering processing is performed, afterwards, an ultrasonic wave signal is moved leftwards, the processed ultrasonic wave signal is multiplied by the transmitted ultrasonic wave signal to obtain a product signal, Fourier transform is performed on the product signal to obtain magnitude spectra of the product signal, the frequency of a peak value in the magnitude spectra is determined by using a maximum value function, and finally the transmission time of the ultrasonic wave in the material to be measured is calculated. According to the method, fast Fourier transform (FFT) and signal multiplication are performed only once, the operation burden is greatly lowered, and thus the method is short in implementation period and high in efficiency; by using chirp signals to excite ultrasonic pulse, the ultrasonic penetrability and the time resolution of ultrasonic detection are improved, and thus the detection accuracy rate is improved.

The invention provides an alcoholic beverage and a preparation method of the alcoholic beverage. The alcoholic beverage with low alcohol content, which is high in content of active ingredients, and sour, sweet and chilly in taste, and has the functions of prevention of oxidation, reduction of blood fat, protection of liver and health care, is obtained by taking roselle and gardenia as raw materials, adding edible alcohol and crystal sugar water, adopting a method of supercritical CO2 extraction-molecular distillation, and optimizing a preparation process.

The invention provides a method and device for selecting an arcing horn and an insulator string in an overhead transmission line. The selection method comprises the following steps: selecting a presetarcing horn according to an impact withstand voltage value which needs to be borne by the arcing horn in the transmission line, and determining the withstand voltage value of the preset arcing horn;obtaining the withstand voltage value of the insulator string according to the given parameters of insulators and the number of the insulators; calculating effectiveness index parameters of the presetarcing horn for the insulator string according to the withstand voltage value of the preset arcing horn and the withstand voltage value of the insulator string; and determining the arcing horn and the insulator string according to the validity index parameters. On the premise of discharging randomness, selection and matching of the arcing horn and the insulator string are facilitated, reference can be provided for selection and matching of the arcing horn and the insulator string under the condition that a complete test is not needed, the efficiency of selecting the arcing horn and the insulator string is improved, the reliability is high, the period of the method is short, and the cost is low.

The invention relates to the technical fields of pesticide and environmental protection, and relates to a byproduct recycling technology in the preparation process of an agricultural bactericide (kresoxim-methyl), in particular to a method for recovering a byproduct (ammonium chloride) in kresoxim-methyl production process. Toluene or petroleum ether is used as an extraction solvent and is mixed with water, a byproduct (crude ammonium chloride) in the kresoxim-methyl production process is used as a raw material, standing and layering are performed after heating, filtrate, which is obtained after the water phase is filtered and subjected to impurity removal, is subjected to cooling crystallization, and finally high-purity ammonium chloride is obtained through centrifugation. The method hasthe advantages of simple process flow, short period, low cost, high product purity (up to 99.6%) and high yield, no solid waste is discharged in industrial production of kresoxim-methyl, and clean production is realized. Meanwhile, the extraction solvent and the ammonium chloride mother liquor can be recycled, and water is saved.

The invention provides a method for preparing astragaloside, and belongs to the technical field of purification of trace components. The method for preparing the astragaloside includes the following steps that an astragalus membranaceus solution containing alcohol extract of astragalus membranaceus is placed in a high performance liquid chromatography column for elution, and when astragaloside appears, fractions of the astragaloside are collected. Through the method, the high purity astragaloside can be obtained, and the method has a short cycle and simple operation.

The invention provides a method for processing meat products by using a high-temperature fermentation technology, and belongs to the field of food processing. The method comprises the following stepsof pretreating raw material meat, mixing the pretreated raw material meat with ingredients, rolling and pickling to obtain pickled meat, fermenting the pickled meat by using a fermenting agent, a black bean peel extract and a cocoa extract to obtain fermented meat, and drying, cooking, cooling, packaging and sterilizing the fermented meat to obtain a fermented meat product. The fermenting agent comprises lactobacillus sake, pediococcus acidilactici, staphylococcus vitulinus and staphylococcus carnosus. The method for processing the meat product by utilizing the high-temperature fermentation technology has the advantages of short period and lower cost, and the prepared meat product is safe, good in flavor and high in nutritional value.

The invention discloses a coating method of a paint containing plant fragrance. The coating method comprises the following steps that S1, the paint containing the plant fragrance is sprayed on the surface of a base material with a spraying thickness of 5[mu] m-10 [mu] m; and S2, the base material sprayed with the paint is heated to 60 DEG C - 120 DEG C, and heat preservation is conducted for 5 min - 10 min. The coating method provided by the invention is easy to coat and easy to adhere to the base material after coating, and the coating method has the advantages of short period and strong adhesive force between the paint and the base material.