A kind of tissue culture method of camphor bilberry
A technology of tissue culture and camphor leaf, applied in the field of plant tissue culture, can solve the problems of scarcity of camphor leaf bilberry resources, etc., and achieve the effect of good application prospect, wide source and low price
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Embodiment 1
[0030] (1) Initiation culture of axillary bud induction
[0031] Obtain the young and tender stems of the camphor bilberry from the vigorous camphor bilberry plant as explants, cut off the leaves of the explants and cut the stems into 4-6cm long stems, and soak them in detergent water for 30-60 minutes , Rinse in running water for 1 to 2 hours and then disinfect. Use a combination of 75% alcohol and 0.1% mercury liters, 75% alcohol soak for 15-30s, 0.1% mercury immersion for 7-12 minutes, and sterile water for 4~ 5 times, cut it into stem segments with 1-2 leaf axils on the sterilized filter paper and absorb the water on the stem segments, then insert the leaf axils upward and obliquely into the axillary bud induction medium (WPM+6-BA2.5mg / L+NAA1.0mg / L+active charcoal 2.5g / L, of which sugar 20~30g / L, agar 4-6g / L, pH5.5), culture temperature is 25±2℃, light intensity is 2000lx, light The cycle ratio is 14h:10h. After 25 days of culture, the bud induction rate was 95%.
[0032] (...
Embodiment 2
[0039] (1) Initiation culture of axillary bud induction
[0040] Obtain the young and tender stems of the camphor bilberry from the vigorous camphor bilberry plant as explants, cut off the leaves of the explants and cut the stems into 4-6cm long stems, and soak them in detergent water for 30-60 minutes , Rinse in running water for 1 to 2 hours and then disinfect. Use a combination of 75% alcohol and 0.1% mercury liters, 75% alcohol soak for 15-30s, 0.1% mercury immersion for 7-12 minutes, and sterile water for 4~ 5 times, cut it into stem segments with 1-2 leaf axils on sterilized filter paper and absorb the water from the stems, and then insert the leaf axils upward and obliquely insert them into the axillary bud induction medium (WPM+6-BA2mg / L +NAA1.5mg / L+activated carbon 3g / L, of which sugar 20~30g / L, agar 4~6g / L, pH5.4), culture temperature is 27±2℃, light intensity is 2500lx, light cycle ratio is 15h : 9h. After 30 days of cultivation, the bud induction rate was 92%.
[004...
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