A kind of tissue culture method of camphor bilberry

A technology of tissue culture and camphor leaf, applied in the field of plant tissue culture, can solve the problems of scarcity of camphor leaf bilberry resources, etc., and achieve the effect of good application prospect, wide source and low price

Active Publication Date: 2016-07-27
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Prior art At present, there is no report on obtaining high-content caffeoylarbutin plant materials by tissue culture technology, and it is necessary to use modern biotechnology to solve the problem of resource scarcity in Vaccinium camphoria

Method used

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  • A kind of tissue culture method of camphor bilberry
  • A kind of tissue culture method of camphor bilberry

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Initiation culture of axillary bud induction

[0031] Obtain the young and tender stems of the camphor bilberry from the vigorous camphor bilberry plant as explants, cut off the leaves of the explants and cut the stems into 4-6cm long stems, and soak them in detergent water for 30-60 minutes , Rinse in running water for 1 to 2 hours and then disinfect. Use a combination of 75% alcohol and 0.1% mercury liters, 75% alcohol soak for 15-30s, 0.1% mercury immersion for 7-12 minutes, and sterile water for 4~ 5 times, cut it into stem segments with 1-2 leaf axils on the sterilized filter paper and absorb the water on the stem segments, then insert the leaf axils upward and obliquely into the axillary bud induction medium (WPM+6-BA2.5mg / L+NAA1.0mg / L+active charcoal 2.5g / L, of which sugar 20~30g / L, agar 4-6g / L, pH5.5), culture temperature is 25±2℃, light intensity is 2000lx, light The cycle ratio is 14h:10h. After 25 days of culture, the bud induction rate was 95%.

[0032] (...

Embodiment 2

[0039] (1) Initiation culture of axillary bud induction

[0040] Obtain the young and tender stems of the camphor bilberry from the vigorous camphor bilberry plant as explants, cut off the leaves of the explants and cut the stems into 4-6cm long stems, and soak them in detergent water for 30-60 minutes , Rinse in running water for 1 to 2 hours and then disinfect. Use a combination of 75% alcohol and 0.1% mercury liters, 75% alcohol soak for 15-30s, 0.1% mercury immersion for 7-12 minutes, and sterile water for 4~ 5 times, cut it into stem segments with 1-2 leaf axils on sterilized filter paper and absorb the water from the stems, and then insert the leaf axils upward and obliquely insert them into the axillary bud induction medium (WPM+6-BA2mg / L +NAA1.5mg / L+activated carbon 3g / L, of which sugar 20~30g / L, agar 4~6g / L, pH5.4), culture temperature is 27±2℃, light intensity is 2500lx, light cycle ratio is 15h : 9h. After 30 days of cultivation, the bud induction rate was 92%.

[004...

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Abstract

The invention discloses a tissue culture method of vaccinium dunalianum containing caffeoyl arbutin, relating to the technical field of tissue culture of plants. A tissue culture seedling culture system is built and the content of caffeoyl arbutin is measured by using improved WPM (woody plant medium) as basal medium at different proportion concentrations of hormone and activated carbon. The growth and root induction of aseptic seedling can be controlled by adjusting the proportion of 6-BA, NAA and the activated carbon. The induction rate of explants reaches 97 percent, the bud seedling is robust, the growth is rapid, and the rooting percentage of the bud seedling is 94%. The method has the advantages of rapid reproduction speed, short period and high content of caffeoyl arbutin, and the like, is an effective way to obtain a large number of caffeoyl arbutin, and provides reliable technical basis for large-scale production of fine rapid propagation seedlings of vaccinium dunalianum.

Description

Technical field [0001] The invention belongs to the technical field of plant tissue culture, and specifically relates to a tissue culture method of camphor leaf bilberry. Background technique [0002] Cinnamomum camphora (Vacciniumdunalianum) is a plant of the genus Vaccinium in the Ericaceae family (Ericaceae), an evergreen shrub. It is mainly produced in Yunnan, Sichuan, Guizhou, Tibet and other places. It is also distributed in Taiwan, Sikkim, Bhutan, India (Northeast), Myanmar (Northeast) and Vietnam. In Yunnan Province, it is mainly distributed in Northwest Yunnan. Central Yunnan Plateau to southern and southeastern Yunnan. Its young leaf buds are cone-shaped like bird's beaks. They are also called "Bird's mouth tea" in Yunnan Yi people. According to records, they have been drunk as a tea substitute for a long time since the Ming Dynasty. effect. Bilberry plants are rich in a variety of nutrients and possess various physiological activities. They are also one of the main ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 赵平唐军荣罗旭璐阚欢刘云李旭
Owner SOUTHWEST FORESTRY UNIVERSITY
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