Preparation method of bovine placenta extract
A technology of bovine placenta and minced meat, applied in skin care preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc. Small, low cost, short cycle effect
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Embodiment 1
[0026] Wash 1 kg of fresh bovine placenta, remove blood and blood vessels, and wash it twice with purified water; add purified water 1.5 times the weight of bovine placenta to the cleaned bovine placenta, and crush it to obtain bovine placenta meat slurry, and control the temperature at 28 ℃; add 0.1% trypsin with the quality of bovine placental minced meat liquid, and then treat at 40°C for 17 hours to obtain the enzymatic solution; centrifuge at 5000r / min for 8min to obtain the supernatant; filter the supernatant through a 0.22μm filter membrane to obtain the filtrate; The filter membrane is ultrafiltered to obtain the collected liquid; finally, the collected liquid is subjected to vacuum freeze-drying to obtain the bovine placenta.
Embodiment 2
[0028] Wash 1 kg of fresh bovine placenta, remove blood and blood vessels, and wash 3 times with purified water; add purified water of 1 times the quality of bovine placenta to the cleaned bovine placenta, and crush it to obtain bovine placenta meat slurry, and control the temperature at 25 ℃; add 0.3% trypsin with the quality of bovine placental minced meat liquid, then treat at 37°C for 4 hours, and let it stand overnight to obtain the enzymolysis solution; centrifuge at 4500r / min for 10 minutes to obtain the supernatant; filter the supernatant through a 0.22 μm filter membrane to obtain the filtrate; Then, the collected solution is obtained by ultrafiltration through a 10kDa ultrafiltration membrane; finally, the collected solution is subjected to vacuum freeze-drying to obtain bovine placenta.
Embodiment 3
[0030] Wash 1kg of fresh bovine placenta, remove blood and blood vessels, wash 3 times with purified water; add purified water twice the weight of bovine placenta to the cleaned bovine placenta, and grind it to obtain bovine placenta meat slurry, and control the temperature at 30 ℃; add 0.6% trypsin with the quality of bovine placental minced meat liquid, then treat at 35°C for 8 hours, and let it stand overnight to obtain the enzymolysis solution; centrifuge at 3000r / min for 15 minutes to obtain the supernatant; filter the supernatant through a 0.22 μm filter membrane to obtain the filtrate; Then, the collected solution is obtained by ultrafiltration through a 10kDa ultrafiltration membrane; finally, the collected solution is subjected to vacuum freeze-drying to obtain bovine placenta.
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