Preparation method of bovine placenta extract

Abstract

The invention discloses a preparation method of bovine placenta, and belongs to the technical field of placenta preparation. In the whole preparation process of the bovine placenta, the activity of active ingredients of the bovine placenta is protected by means of temperature control homogenization, enzymolysis and the like, so that the conformation change of the active ingredients in the bovine placenta is effectively avoided, and the bovine placenta has relatively high activity; by adopting the means of centrifugation, filtration and ultrafiltration, impurities are removed, effective components are intercepted, and the produced bovine placenta is small in molecular weight and convenient to absorb by human bodies. The method provided by the invention is short in period, the produced bovine placenta is high in purity and high in activity, the main component is a small molecular peptide fragment, the peptide content of 100-1000Da reaches 95% or above, and 0.05 mg / mL of the bovine placenta can play a role in promoting proliferation of HFF-1 (human skin fibroblasts). The whole production process is pollution-free, free of toxic and side effects, low in cost and good in application prospect.

Description

technical field

[0001] The invention relates to a preparation method of bovine placenta, belonging to the technical field of placenta preparation. Background technique

[0002] Placenta, also known as "Zichehe", has been regarded as an elixir since ancient times, and is generally used as a medicine for medical treatment, health care, beauty and anti-aging. Modern medical pharmacological research and clinical trials also show that placenta can increase microcirculation, improve metabolism, regulate and balance endocrine, repair injured skin tissue, dissolve aging cutin, and beautify skin. Placenta contains amino acids, proteins, sugars, nucleic acids, minerals, vitamins, growth factors, etc. Among them, polypeptides are better than macromolecular proteins, which are easier for the body to absorb, and better than amino acids, which are easier to be absorbed by the body in a complete form, and are also activated Basic elements of cells. Therefore, the extraction of bovine pla...

Examples

Embodiment 1

[0026] Wash 1 kg of fresh bovine placenta, remove blood and blood vessels, and wash it twice with purified water; add purified water 1.5 times the weight of bovine placenta to the cleaned bovine placenta, and crush it to obtain bovine placenta meat slurry, and control the temperature at 28 ℃; add 0.1% trypsin with the quality of bovine placental minced meat liquid, and then treat at 40°C for 17 hours to obtain the enzymatic solution; centrifuge at 5000r / min for 8min to obtain the supernatant; filter the supernatant through a 0.22μm filter membrane to obtain the filtrate; The filter membrane is ultrafiltered to obtain the collected liquid; finally, the collected liquid is subjected to vacuum freeze-drying to obtain the bovine placenta.

Embodiment 2

[0028] Wash 1 kg of fresh bovine placenta, remove blood and blood vessels, and wash 3 times with purified water; add purified water of 1 times the quality of bovine placenta to the cleaned bovine placenta, and crush it to obtain bovine placenta meat slurry, and control the temperature at 25 ℃; add 0.3% trypsin with the quality of bovine placental minced meat liquid, then treat at 37°C for 4 hours, and let it stand overnight to obtain the enzymolysis solution; centrifuge at 4500r / min for 10 minutes to obtain the supernatant; filter the supernatant through a 0.22 μm filter membrane to obtain the filtrate; Then, the collected solution is obtained by ultrafiltration through a 10kDa ultrafiltration membrane; finally, the collected solution is subjected to vacuum freeze-drying to obtain bovine placenta.

Embodiment 3

[0030] Wash 1kg of fresh bovine placenta, remove blood and blood vessels, wash 3 times with purified water; add purified water twice the weight of bovine placenta to the cleaned bovine placenta, and grind it to obtain bovine placenta meat slurry, and control the temperature at 30 ℃; add 0.6% trypsin with the quality of bovine placental minced meat liquid, then treat at 35°C for 8 hours, and let it stand overnight to obtain the enzymolysis solution; centrifuge at 3000r / min for 15 minutes to obtain the supernatant; filter the supernatant through a 0.22 μm filter membrane to obtain the filtrate; Then, the collected solution is obtained by ultrafiltration through a 10kDa ultrafiltration membrane; finally, the collected solution is subjected to vacuum freeze-drying to obtain bovine placenta.

technical field

[0001] The invention relates to a preparation method of bovine placenta, belonging to the technical field of placenta preparation. Background technique

[0002] Placenta, also known as "Zichehe", has been regarded as an elixir since ancient times, and is generally used as a medicine for medical treatment, health care, beauty and anti-aging. Modern medical pharmacological research and clinical trials also show that placenta can increase microcirculation, improve metabolism, regulate and balance endocrine, repair injured skin tissue, dissolve aging cutin, and beautify skin. Placenta contains amino acids, proteins, sugars, nucleic acids, minerals, vitamins, growth factors, etc. Among them, polypeptides are better than macromolecular proteins, which are easier for the body to absorb, and better than amino acids, which are easier to be absorbed by the body in a complete form, and are also activated Basic elements of cells. Therefore, the extraction of bovine pla...

Examples

Embodiment 1

[0026] Wash 1 kg of fresh bovine placenta, remove blood and blood vessels, and wash it twice with purified water; add purified water 1.5 times the weight of bovine placenta to the cleaned bovine placenta, and crush it to obtain bovine placenta meat slurry, and control the temperature at 28 ℃; add 0.1% trypsin with the quality of bovine placental minced meat liquid, and then treat at 40°C for 17 hours to obtain the enzymatic solution; centrifuge at 5000r / min for 8min to obtain the supernatant; filter the supernatant through a 0.22μm filter membrane to obtain the filtrate; The filter membrane is ultrafiltered to obtain the collected liquid; finally, the collected liquid is subjected to vacuum freeze-drying to obtain the bovine placenta.

Embodiment 2

[0028] Wash 1 kg of fresh bovine placenta, remove blood and blood vessels, and wash 3 times with purified water; add purified water of 1 times the quality of bovine placenta to the cleaned bovine placenta, and crush it to obtain bovine placenta meat slurry, and control the temperature at 25 ℃; add 0.3% trypsin with the quality of bovine placental minced meat liquid, then treat at 37°C for 4 hours, and let it stand overnight to obtain the enzymolysis solution; centrifuge at 4500r / min for 10 minutes to obtain the supernatant; filter the supernatant through a 0.22 μm filter membrane to obtain the filtrate; Then, the collected solution is obtained by ultrafiltration through a 10kDa ultrafiltration membrane; finally, the collected solution is subjected to vacuum freeze-drying to obtain bovine placenta.

Embodiment 3

[0030] Wash 1kg of fresh bovine placenta, remove blood and blood vessels, wash 3 times with purified water; add purified water twice the weight of bovine placenta to the cleaned bovine placenta, and grind it to obtain bovine placenta meat slurry, and control the temperature at 30 ℃; add 0.6% trypsin with the quality of bovine placental minced meat liquid, then treat at 35°C for 8 hours, and let it stand overnight to obtain the enzymolysis solution; centrifuge at 3000r / min for 15 minutes to obtain the supernatant; filter the supernatant through a 0.22 μm filter membrane to obtain the filtrate; Then, the collected solution is obtained by ultrafiltration through a 10kDa ultrafiltration membrane; finally, the collected solution is subjected to vacuum freeze-drying to obtain bovine placenta.