Method of establishing animal paternity identification system on basis of whole genome STR

A whole-genome, paternity testing technology, applied in the field of paternity testing system establishment, can solve the problems that cannot be ruled out, no public reports, etc., and achieve the effect of low cost, high screening throughput and short cycle

Active Publication Date: 2015-04-01
BGI FORENSIC TECH (SHENZHEN) CO LTD
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0007] 2. In the case that certain marker genes of the offspring are from the biological father, and it is assumed that the father also carries these genes, it cannot be ruled out that it is the biological father of the offspring
[0010] Through literature search, do not see the public report identical with the present invention

Method used

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  • Method of establishing animal paternity identification system on basis of whole genome STR
  • Method of establishing animal paternity identification system on basis of whole genome STR
  • Method of establishing animal paternity identification system on basis of whole genome STR

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0036] Embodiment: the establishment of Crested Ibis STR paternity test system

[0037] Specific steps:

[0038] 1) Define a tandem repeat sequence with a repeat unit of 1 to 6 bp and a total length of not less than 15 bp as STR.

[0039] Tandem Repeat Finder (TRF) was used to search for STRs in the Crested Ibis genome. The search program parameters are set as follows: "Match=2, Mismatch=7, Delta=7, PM=80, PI=10, Minscore=30, Maxperiod=6". A total of at least 820,000 STR loci have been found in the Crested Ibis genome, including about 50,000 STR loci with 4 base repeats.

[0040] 2) Select 300 STR sites from the STR sites with a repeat unit of 4 bp.

[0041] Selection principles: i) the locus is a non-degenerate STR locus; ii) each locus is inherited independently.

[0042] 3) Use the primer design software Primer Premier 5.0 to design upstream and downstream primers in the conserved sequences within 200 bp at both ends of the STR. The design parameters of the primers wer...

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Abstract

The invention relates to the field of genetic resource conservation, particularly relates to the field of animal paternity identification, and discloses a method of establishing an animal paternity identification system on basis of whole genome STR. The method comprises the following steps: 1) looking up whole genome STR loci; 2) preliminarily screening the STR loci; 3) carrying out a primer design; 4) randomly sampling animal populations and extracting genome DNA; 5) verifying the population diversity of the STR loci; 6) carrying out Hardy-Weinberg equilibrium inspection on the STR loci with genetic polymorphism, and screening out the STR loci with the genotype frequency distribution which meets the Hardy-Weinberg equilibrium; 7) evaluating the forensic medicine application value of the STR loci; 8) establishing the STP animal paternity identification system. The method is convenient to operate; the constructed paternity identification system has a paternity exclusion probability of more than 0.99, and is capable of accurately carrying out animal paternity identification, therefore, the paternity identification system can be used for guiding the breeding of rare species, and the identification and breeding of economic species.

Description

technical field [0001] The invention relates to a method for establishing a parentage identification system, in particular to a method for establishing an animal parentage identification system based on STR sites in the whole genome sequence. Background technique [0002] Whether it is the protection of rare animal germplasm resources or the breeding of animals with high economic value, there are more and more events requiring the identification and paternity of animal individuals. For example, paternity testing and individual identification of giant pandas can help avoid the problem of inbreeding in captivity, which is of great significance to saving the giant panda species. As another example, in the modern horse breeding and horse racing industry, the birth source identification of some excellent breeds of horses is also highly valued. [0003] Because DNA molecular markers can directly reflect the changes and differences of the genetic material itself or the genome, and...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156
Inventor 李生斌李波刘清波常辽熊子军
Owner BGI FORENSIC TECH (SHENZHEN) CO LTD
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