50 results about "Genotype Assay" patented technology

The present invention provides fluorescence-based real-time PCR assays for the rapid detection of β₂-adrenergic receptor single nucleotide polymorphisms (SNPs). The genotyping assay can be used to detect SNPs of human β₂-adrenergic receptor (β₂-AR) single nucleotide polymorphisms A46G and C79G.

Genetic polymorphisms responsible or associated with altered expression of cytochrome P450 CYP3A5 enzyme are described. Single nucleotide polymorphisms are provided. Methods for identifying subjects having a low or high drug metabolizing phenotype associated with CYP3A5 expression are provided. Assays, kits and methods for determining and assaying the CYP3A5 genotype and phenotype of individual patients are disclosed. Oligonucleotide probes and primers for use in the assays, kits and methods are described. Assays and methods for determining and evaluating an individual's metabolism of drugs and therapeutic agents, the potential for drug interactions, and thereby toxicity and effectiveness of certain drugs and treatment modalities, are provided.

The invention discloses a composite PCR amplification system and an assay kit for genotyping of an STR mark detection system for individual identification and paternity test of yaks. The primer with afluorescence mark can achieve amplification of 15 STR mark sites of yaks, the mark sites are unlocked and have high heterozygosity and rich content of polymorphism information, and amplified productsare of right size. According to a detection method, genotyping results of 15 STR sites are obtained through amplification of 15 pairs of primers and electrophoresis detection; and individual identification and paternity test of yaks are carried out according to the STR locus genotyping results and gene frequency of each STR of yaks. Since individual identification and paternity test of yaks can be carried out effectively by comprehensively analyzing the STR locus genotyping results, accuracy of genealogy in yak breeding can be improved and the breeding process can be accelerated; accordingly,the composite PCR amplification system and the assay kit have good application prospect and economic value.

The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. The present invention also provides cytochrome p450 genotyping methods and reagents for genotyping assays and a genomic DNA copy number assays.

High yield sesame plants and parts thereof are provided. Phenotypic and genotypic analysis of many sesame varieties was performed to derive markers for phenotypic traits that contribute to high yield, and a breeding simulation was used to identify the most common and most stable markers. Examples for such phenotypic traits include the number of capsules per leaf axil, the capsule length, the height to first capsule and the number of lateral shoots. Following verification of trait stability over several generations, markers and marker cassettes were defined as being uniquely present in the developed sesame lines. The resulting high yield, shatter-resistant sesame lines can be used to increase sesame yield for its various uses.

The invention provides a molecular marker method for rapidly identifying suitability of tea germplasms for manufacture of oolong. The method comprises steps as follows: (1) extracting genome DNA of to-be-identified tea germplasms; (2) synthesizing a specific SSR fluorescence-labeled primer; (3) taking the genome DNA of the to-be-identified tea germplasms as a template and performing PCR (polymerase chain reaction) amplification with the specific SSR fluorescence-labeled primer; (4) detecting a PCR amplification product and acquiring genotypes at specific sites of the to-be-identified tea germplasms; (5) analyzing the genotypes of the to-be-identified tea germplasms. The tea germplasms suitable for manufacture of the oolong can be screened out through simple experimental operation and analysis, and only 1-2 workdays are spent on the whole process; the method is used for molecular marker-assisted selection in oolong varieties, directive breeding of oolong varieties can be realized to a certain extent, and the blindness of new oolong variety selection is reduced; genetic backgrounds and relationships among multiple to-be-identified tea germplasms can be mastered preliminarily with the method.

The hybridization matching technique of green case egg breeded by the duck, is a breeding seed selection technique of poultry high quality species. The species is the high oil duck, people centralize the individual and do group feeding to breed. Detect the genetype of the male duck in the group, the determination is letting the male duck and the female duck which breed white case egg cross, if the descendants all breed green case eggs, the male duck is the green case gene homozygous type; if the descendants breed green and white case eggs, the male duck is the gene heterozygous type; If the descendants all breed white case eggs, the male duck is the white case gene homozygous type. Select the green case gene homozygous male ducks to match with the female ducks which breed green case eggs, we will get the green case homozygous group. Take it as the paternal after the selection of performance, then match with other groups, the descendants will all be the ducks that breed green case eggs. The invention adopts quality description genetic principle to improve the breed and quality of the duck, it is simple, scientific, advanced, and with great spread application value.

The present invention provides methods for optimizing nucleic acid detection assays for use in basic research and clinical research. Specifically, the invention provides a method for empirically optimizing nucleic acid probes by testing them with samples containing genomic DNA with variations in copy number in different regions of the genome. The invention enables the optimization of probes for any hybridization based assay including microarrays, bead-based assays, genotyping assays and RNAi assays.

Sesame plants with shattering-resistant capsules. and parts thereof are provided. Phenotypic and genotypic analysis of many sesame varieties was performed to derive markers for phenotypic traits that contribute to shattering-resistance, and a breeding simulation was used to identify the most common and most stable markers. Following verification of trait stability over several generations, markers and marker cassettes were defined as being uniquely present in the developed sesame lines. The resulting shatter-resistant sesame lines can be used to increase sesame productivity for its various uses.

Sesame plants with high oil content and/or high yield, and parts thereof are provided. Phenotypic and genotypic analysis of many sesame varieties were performed to derive markers for phenotypic traits that contribute to high oil content and/or high yield, and a breeding simulation was used to identify the most common and most stable markers. Examples for such phenotypic traits include the oil content as measured by near infrared spectroscopy, and yield traits related to plant morphology, number of capsules and the size of the seeds. Following verification of trait stability over several generations, markers and marker cassettes were defined as being uniquely present in the developed sesame lines. The resulting high oil content and/or high yield, shatter-resistant sesame lines can be used to increase sesame oil production for its various uses.

This invention relates to antiviral drug susceptibility and resistance tests to be used in identifying effective drug regimens for the treatment of human immunodeficiency virus (HIV) infection and acquired immunodeficiency syndrome (AIDS) and further relates to the means and methods of monitoring the clinical progression of HIV infection and its response to antiretroviral therapy, particularly non-nucleoside reverse transcriptase inhibitor therapy using phenotypic susceptibility assays or genotypic assays.

The invention discloses a masson pine SNP (single nucleotide polymorphism) genotyping method based on the HRM (high-resolution melt) technology, and belongs to the technical field of genetic engineering. The masson pine SNP genotyping method includes screening ten SNP loci, designing corresponding primers, extracting genomic DNA of masson pines, carrying out PCR reaction and HRM analysis, collecting data, performing SNP locus genotype analysis on each sample, and creating SNP fingerprints of the masson pine samples by the method. The masson pine SNP genotyping method has the advantages of simplicity, convenience and rapidness in operation, low cost, accurate result, and capability of being applied to molecular marker assisted breeding and genetic resource diversity research.

This invention relates to antiviral drug susceptibility and resistance tests to be used in identifying effective drug regimens for the treatment of human immunodificiency virus (HIV) infection and acquired immunodeficiency syndrome (AIDS) and further relates to the means and methods of monitoring the clinical progression of HIV infection and its response to antiretroviral therapy, particularly non-nucleoside reverse transcriptase inhibitor therapy using phenotypic susceptibility assays or genotypic assays.

The present disclosure provides a method for differentiating myometrial tumors/uterine neoplasms such as LM, LMS and IMT. Further, the disclosure provides a method for treating a uterine leiomyoma in a subject, comprising: (a) performing a genotyping assay on a biological sample from the subject to determine whether the subject has a uterine leiomyosarcoma genotype, and (b) surgically removing the uterine leiomyoma if the subject does not have a uterine leiomyosarcoma genotype.

The invention belongs to the field of identification and utilization of tea tree germplasm resources and new variety selection and breeding, and specifically relates to a molecular marker method for quickly identifying tea tree germplasm with early bud and leaf growth. A pair of dCAPS marker primers are designed by using the DNA sequence near the SNP marker. Different tea tree germplasm DNA can be screened out by using primers for PCR amplification, electrophoresis separation and genotype analysis, which realizes the molecular marker-assisted breeding of tea tree varieties with early bud and leaf growth.

The invention relates to a new bidirectional one-step genotyping method through PCR. The invention adopts the following technical scheme: a) designing a 5' terminal template mismatched or 5' terminal closed specific PCR primer according to the genotype of an SNP locus; b) adopting the 5' terminal template mismatched or 5' terminal closed specific PCR primer to simultaneously carry out PCR toward two directions respectively from the SNP locus; c) using an external terminal primer at the far terminal of the SNP locus to control the length of the PCR products; and d) detecting the PCR products in two directions according to the difference of the length of the PCR products in two directions and the difference of DNA sequences, thus obtaining the SNP result. The method designs the specific primer aiming at the genotype mainly related to the shape and can simultaneously determine the genotypes of a single or a plurality of loci by PCR. Each electrophoretic band represents the genotype of a locus. The results of the genotypes in different positions can be given by the integral electrophoregram.