15 results about "Genotype Assay" patented technology

The present disclosure relates generally to compositions, systems, and methods for lysing blood samples comprising an infectious agent, for example a bacteria, or a fungus, while maintaining viability of the infectious agent for subsequent characterization, for example phenotyping or genotyping. A lysing composition may include one or more surfactants, such as one or more detergents. A lysing composition may include lysing agent including one or more salts. A lysing composition may include one or more enzymes to break down one or more lyses byproducts, such as nucleic acids and / or protein aggregates.

The application discloses a kind of high yield high-quality efficient rice variety comprehensive screening method, it is related to rice variety screening technical field, including determining ecological point and collecting environmental data, according to different ecological point selection different variety group and carry out genotypic analysis;Collect the phenotypic data of rice variety, establish the correlation model between genotypic data and phenotypic data, screen high-quality genes;According to the selection result of screening, the key genes of target variety and directional editing rice variety are selected, the gene editing effect is evaluated, and the performance of rice variety is further verified after gene editing, and the variety screening is carried out.The application can not only effectively integrate ecological data, gene data and phenotype data for comprehensive evaluation, optimize the problem of rice variety from the global perspective, but also improve the actual performance of rice variety through precision cultivation management, and lay the foundation for the variety layout of large-area rice yield, quality and efficiency.

The invention provides a primer probe group for noninvasive detection of gene polymorphism of a placenta drug transporter. The primer probe group comprises a primer probe group for detecting c.80G > A polymorphism of an RFC1 gene or / and c.1236Cgt of an ABCB1 gene, a primer probe set of T polymorphism; the method for noninvasive detection of placenta drug transporter gene polymorphism comprises the following steps: taking peripheral blood free DNA (deoxyribonucleic acid) of a pregnant woman in a gestation period as a template, and performing micro-droplet generation and PCR (polymerase chain reaction) amplification by using a primer probe group to obtain an RFC1 gene c.80Ggt; a or / and an ABCB1 gene c.1236Cgt; and carrying out genotype analysis on copy number information of T mutation sites. The invention further provides application of the primer probe set for noninvasive detection of placenta drug transporter gene polymorphism, and the primer probe set is used for preparing a kit for noninvasive detection of placenta drug transporter gene mutation site genotypes. According to the invention, the peripheral blood DNA of the pregnant woman in the gestation period is detected by using digital PCR, the placenta drug transporter gene mutation information is noninvasively obtained, the transplacental transport of the drug and the possible influence on the fetus are evaluated, and an important reference is provided for the treatment decision of the pregnancy complication / complication and the like.

The presently described and claimed technology relates to high-throughput automated methods for the investigation of ApoE isoforms the subsequent prediction of a likelihood of developing a neurodegenerative disease.

The present application belongs to the technical field of molecular biology, and particularly relates to a primer combination for identifying peony resources and application thereof. The present application aims to provide a new method for identifying peony resources. The technical scheme of the present application is a primer combination for identifying peony resources. The present application takes the progeny germplasm resources obtained by natural pollination of Central Plains peony and Northwest peony as materials, and uses 57 pairs of SSR marker primers for screening. Finally, 9 core SSR markers are screened for genotype analysis, and a molecular identity card system is successfully constructed for 190 peony germplasms by using a coding mode combining numbers and uppercase and lowercase English letters.

Sesame plants with high protein content and / or improved taste, as well as seeds thereof and food products made therefrom are provided. Phenotypic and genotypic analysis of many sesame varieties were performed followed by analysis to derive markers for phenotypic traits that contribute to high protein content and / or improved taste, and a breeding simulation was used to identify the most common and most stable markers. Following verification of trait stability over several generations, markers and marker cassettes were defined as being uniquely present in the developed sesame lines. Various food products made of seeds of the disclosed varieties have higher protein content and improved taste compared to products made of commercial sesame varieties.

The utility model discloses a hepatitis C genetic typing detection experiment device which comprises a machine box, a transparent experiment box is fixed on one side of the machine box, ultraviolet sterilizing lamps are installed at the two ends inside the transparent experiment box respectively, operation openings are evenly formed in one side of the transparent experiment box, and the operation openings are connected with the machine box. A heat preservation storage groove and a PLC are mounted on one side, far away from the transparent experiment box, of the case. According to the utility model, the low-temperature storage cavity and the like are arranged; a user can respectively clamp a test tube filled with a hepatitis C genotyping detection reagent and some test tubes filled with samples to be detected into detection reagent storage grooves in the room-temperature storage cavities or the two low-temperature storage cavities with different temperatures according to the temperature requirements of a storage environment; and then the hepatitis C genotyping test paper is clamped into two test paper storage grooves in the room-temperature storage cavity for storage, so that the device has the advantage of classified storage, and experimental articles are convenient to store and flexible to take and use.

The invention relates to the technical field of gene engineering, in particular to application of a CmESI gene in regulating and controlling dwarfing of a muskmelon plant. According to the invention, methods of genetic analysis, mixed pool sequencing, Mutmap and Kasp genotype analysis and the like are combined to locate a gene causing extreme dwarfing of a plant, an SNP variation site of G / A existing in a first basic group of a seventh exon of a CmESI gene in a mutant esi is found out, the variation site causes CmESI to generate a variable shearing form with a retained sixth intron, and the mutation site is used for detecting the extreme dwarfing of the plant. A termination codon is formed in advance and cannot be translated into complete protein, so that the CmESI gene loses functions; the expression of CmESI in the 13C germplasm is inhibited by using a virus-induced gene silencing technology, and the plant height of a TRSV-CmESI plant is obviously lower than that of a control group plant; therefore, the muskmelon CmESI gene has certain theoretical significance and practical value in regulating and controlling the height of muskmelon plants.

The embodiment of the invention discloses a helicobacter pylori detection primer group based on a targeted high-throughput sequencing technology, a kit and a detection method and application of the helicobacter pylori detection primer group. The primer group in the embodiment of the invention comprises specific primer pairs aiming at mutation sites of Hp species identification genes, subtype identification genes and various drug resistance related genes and human CYP2C19 genotyping sites. Through an innovative primer design strategy, a tNGS detection system constructed by using the primer group can simultaneously complete Hp infection confirmation, pathogenic subtype distinguishing, drug resistance evaluation of six common antibiotics and host proton pump inhibitor metabolic capability (CYP2C19 genotype) analysis in single detection; and a comprehensive and efficient solution is provided for precise diagnosis and individualized medication of Hp infection.

Pea plants or part(s) thereof, as well as products such as commodity whole or split grains, pea protein concentrates (PPC), dairy analogues or protein-fortified beverages and meat analogues or texturized pea protein prepared therefrom, which have high protein content, low fat content and neutral taste are provided. The pea plans, part(s) and products are characterized by specific sets of genetic markers that correspond to identified quantitative trait loci (QTLs). Phenotypic and genotypic analysis of many pea varieties was performed to derive the markers for these phenotypic traits, and a breeding simulation was used to identify the most common and most stable markers. Following verification of trait stability over several generations, markers and marker cassettes were defined as being uniquely present in the developed pea lines. The resulting pea lines can be used to provide products with pea-based protein and low or no off flavors.

The present invention includes methods for treating a human patient with Crenolanib, wherein the human patient is suffering from a FLT3 mutated leukemia, the method comprising: determining that the human patient has a poor prognosis by: obtaining or having obtained a leukemia biological sample and performing or having performed a genotyping assay on the biological sample to determine that the human patient has both a mutated FLT3 or a constitutively active FLT3 mutant and one or more driver mutations in one or more epigenetic regulator proteins that results in a loss of normal function of the epigenetic regulator proteins which, indicates that the patient has a poor prognosis; and administering to the patient determined to have the poor prognosis a therapeutically effective amount of Crenolanib, or a pharmaceutically acceptable salt thereof having the formula:to treat the leukemia.

Disclosed is a configuration for determining a genotyping label composition of a target individual using direct acyclic paths. The configuration includes receiving a phased genotype of the target individual, including a first haplotype and a second haplotype. The configuration initiates a full-ethnicity hidden Markov model (HMM) including nodes with a set of ethnicity labels. The first haplotype is input to determine a first subset of ethnicity labels that match the first haplotype. The second haplotype is input to determine a second subset of ethnicity labels that match the second haplotype. The first and second subsets of ethnicity labels are combined to create a candidate subset of ethnicity labels for the target individual. The configuration initiates a simplified HMM with nodes from the candidate subset of ethnicity labels. The phased genotype of the target individual is input to the simplified HMM to determine genotyping label composition of the target individual.

The present disclosure relates generally to compositions, systems, and methods for lysing blood samples comprising an infectious agent, for example a bacteria or a fungus, while maintaining viability of the infectious agent for subsequent characterization, for example phenotyping or genotyping. A lysing composition may include one or more surfactants, such as one or more detergents. A lysing composition may include lysing agent including one or more salts. A lysing composition may include one or more enzymes to break down one or more lyses byproducts, such as nucleic acids and / or protein aggregates.

The invention relates to the technical field of agricultural biology, and discloses a method for identifying rice substantive derived varieties based on a whole genome SNP site set. The method comprises the following steps: carrying out whole genome sequencing and genotype analysis on a rice variety to be detected and a control variety, calculating the genetic similarity between the varieties by utilizing a set of pre-screened 15,772 core SNP site sets, and setting a unified judgment threshold value for indica rice and japonica rice subgroups based on the distribution of the site sets in a large-scale group. Therefore, the defects that in the prior art, site coverage is limited, steps are tedious, and subgroup genetic structure interference is caused are overcome, and efficient, stable and cross-subgroup applicable accurate judgment on rice substantive derived varieties is achieved.