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Method for paternity test through utilizing specific single nucleotide polymorphism (SNP) combination

A paternity test and genomics technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems affecting the accuracy and repeatability of paternity test, difficulty in distinguishing and judging types, etc., and achieve a simple human paternity test. , the result is accurate

Inactive Publication Date: 2013-03-20
上海迪道科技有限公司 +1
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the sequence lengths of different alleles of STR are similar, so it is difficult to distinguish and judge the type. Generally, the error rate of each PCR reaction can reach 1%~5% (bonin et al. 2004; Weller et al. 2004), Type errors affect the accuracy and reproducibility of paternity tests

Method used

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  • Method for paternity test through utilizing specific single nucleotide polymorphism (SNP) combination
  • Method for paternity test through utilizing specific single nucleotide polymorphism (SNP) combination

Examples

Experimental program
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Embodiment 1

[0014] Example 1: Using flight mass spectrometry to perform SNP typing and parent-child inference on the population:

[0015] 1) Genomic DNA extraction

[0016] 1) The samples in this test are oral mucosal cells collected with a scraper and stored at room temperature. The specific steps of DNA extraction by scraping stick sampling are as follows:

[0017] 2) Dissolve the exfoliated cells on the oral scraper in 400uL 1X lysate, add 1uL proteinase K, and bathe in 56°C water for 30 minutes;

[0018] 3) Take out the sample and place it in an ice-water bath for 1 minute, add 150uL of 6mol / L NaCl, shake vigorously for 15-20 seconds, and centrifuge at 13000 rpm for 10 minutes;

[0019] 4) Pipette the supernatant into a new centrifuge tube, add 1.1mL of absolute ethanol, turn it upside down 10 times until flocculent DNA precipitates, place at room temperature for 10 minutes, centrifuge at 13,000 rpm for 2 minutes to precipitate DNA;

[0020] 6) Discard the supernatant, then ...

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Abstract

The invention discloses a method for a paternity test through utilizing a specific single nucleotide polymorphism (SNP) mark combination which comprises 60 SNP marks. The nucleotide sequences of the forward primers of the SNP marks are shown as SEQ ID NO.1-60; the nucleotide sequences of downstream primers are shown as SEQ ID NO.61-120; and the nucleotide sequences of single base extension primers are shown as SEQ ID NO.121-180. The method utilizes a flight mass spectrum method, the SNP mark combination is used for human paternity tests, and the method has the advantages of accurate result, quickness and convenience in method and high flux.

Description

technical field [0001] The invention relates to a detection system for human paternity identification using SNP markers, in particular to 60 marker combinations, primer sequences and detection methods. Background technique [0002] The modern method of kinship identification is DNA analysis, which uses genetic markers on human chromosomes to identify whether two individuals are related by blood. The probability of sharing the same marker between related individuals is greater than that of unrelated individuals. At present, the genetic marker used for paternity identification is the second-generation genetic marker short tandem repeat sequence (STR), which has the characteristics of wide distribution, easy detection, large amount of information, high polymorphism, and Mendelian co-dominant inheritance. . When STR conducts paternity testing, generally more than a dozen STR sites are detected. If more than 3 sites are different, parent-child relationship can be ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 王健其他发明人请求不公开姓名
Owner 上海迪道科技有限公司
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