Identifying method of megalobrama amblycephala family by microsatellite
An identification method and a technology for bream, which are applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve problems such as restricting the development of fine-breed selection and breeding, and achieve the effects of saving manpower, saving costs, and avoiding cumbersome operations.
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[0074] The following are specific embodiments of the present invention:
[0075] 1) Breeding of bream family and polyculture of offspring
[0076] Selected 44 excellent female parents and 34 male parents from the natural groups of bream (silt lake and Liangzi Lake in Hubei Province, Poyang Lake in Jiangxi Province), and artificially induced labor by injecting hormones. The hormone injection method is as follows: first In the first injection, only female fish were injected, and the injection dose was 1 mg / kg body weight of luteinizing hormone releasing hormone A2 (LRH-A2); the second injection was carried out about 12 hours after the first injection, and both male and female bream were injected The drugs for injection were luteinizing hormone releasing hormone A2 and dioconone (DOM), and the injection doses were 4 mg / kg body weight and 5 mg / kg body weight, respectively. 6-8 hours after the second injection, artificial insemination was carried out, and male and female half-sib ...
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