Identifying method of megalobrama amblycephala family by microsatellite

An identification method and a technology for bream, which are applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve problems such as restricting the development of fine-breed selection and breeding, and achieve the effects of saving manpower, saving costs, and avoiding cumbersome operations.

Inactive Publication Date: 2012-09-05
HUAZHONG AGRI UNIV
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Problems solved by technology

Therefore, it has always been a difficult problem to identify fish individuals and family identification, which largely limits the development of improved species breeding.
So far, there is no report on the application of microsatellite markers in the identification of bream families

Method used

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  • Identifying method of megalobrama amblycephala family by microsatellite
  • Identifying method of megalobrama amblycephala family by microsatellite
  • Identifying method of megalobrama amblycephala family by microsatellite

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Embodiment Construction

[0074] The following are specific embodiments of the present invention:

[0075] 1) Breeding of bream family and polyculture of offspring

[0076] Selected 44 excellent female parents and 34 male parents from the natural groups of bream (silt lake and Liangzi Lake in Hubei Province, Poyang Lake in Jiangxi Province), and artificially induced labor by injecting hormones. The hormone injection method is as follows: first In the first injection, only female fish were injected, and the injection dose was 1 mg / kg body weight of luteinizing hormone releasing hormone A2 (LRH-A2); the second injection was carried out about 12 hours after the first injection, and both male and female bream were injected The drugs for injection were luteinizing hormone releasing hormone A2 and dioconone (DOM), and the injection doses were 4 mg / kg body weight and 5 mg / kg body weight, respectively. 6-8 hours after the second injection, artificial insemination was carried out, and male and female half-sib ...

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Abstract

The invention belongs to the technical field of fish breeding, relates to the technical field of molecular markers, and particularly relates to a method for accurately identifying a megalobrama amblycephala polyculture family by utilizing a microsatellite marker. The method comprises the following steps: (1) adopting an artificial propagation method for breeding a megalobrama amblycephala family,and breeding 54 family progenies in a same pond for polyculture; (2) adopting a fluorescent marker primer and a nested PCR amplification method for analyzing genotypes of a parent at 12 microsatellite sites, and screening out effective microsatellites suitable for family identification; (3) analyzing the genotypes of the family progenies in polyculture at the effective microsatellite sites; and (4) identifying parent sources of all the progenies. In the invention, a paternity test method is established for megalobrama amblycephala for the first time, with the identification accuracy rate up to 98.4%. Through the family screening and identification, the phenomenon of inbreeding existing in the breeding of megalobrama amblycephala in groups can be reduced so as to be more advantageous to the realization of family breeding, speed up the process of breeding, and improve the breeding effect.

Description

technical field [0001] The invention relates to the technical field of fish breeding and molecular markers, in particular to a method for using microsatellite markers to identify the progeny parents of the group bream polyculture family. Background technique [0002] Giant bream (Megalobrama amblycephala Yih, 1955), also known as Wuchang fish, belongs to Osteichthyes, Cypriniformes, Cyprinidae, and genus Bream. Its natural distribution is limited to a few lakes in the middle and lower reaches of the Yangtze River. Bory bream is one of the important herbivorous economic fishes in my country. Due to its wide feeding habits, low cost, fast growth, high survival rate, and easy fishing, it can lay eggs and reproduce in ponds. It has the advantages of high rate, good shape, and moderate size, so it is widely promoted as an excellent herbivorous fish species throughout the country. [0003] In recent years, grouphead bream has become a best-selling species in the market, and the ec...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 高泽霞王卫民曾聪罗伟王焕岭
Owner HUAZHONG AGRI UNIV
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