Oval pompanos trevally family paternity test method

A technology of oval pomfret and pomfret, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of inapplicable 1-to-1 production mode, difficulties, and small number of families, and achieve saving Management and breeding costs, improved accuracy, and cost-saving effects

Active Publication Date: 2015-06-10
HAINAN UNIVERSITY
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Problems solved by technology

The number of families established in this way is small, and the individual management of each family is greatly affected by the environment, which is not conducive to family selection; and it is very difficult to physically mark individuals for mixed families in the case of many families
At present, it is difficult to judge the reproductive maturity and sex of pomfret ovata, and the 1-to-1 production method is not applicable.
Therefore, the establishment and identification of the family of Pomfret ovata has always been a difficult problem, which hinders the breeding work of Pompano ovata to a large extent.
So far there is no report on the use of microsatellite markers in the family identification of Pompano ovata

Method used

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  • Oval pompanos trevally family paternity test method
  • Oval pompanos trevally family paternity test method
  • Oval pompanos trevally family paternity test method

Examples

Experimental program
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Embodiment Construction

[0027] 1) Breeding of pomfret family and offspring breeding

[0028] 87 excellent individuals were selected from the natural population of Pomfret ovata (Hainan area) as parents, electronic markers were implanted in their back muscles and the growth traits of each individual were measured. A small piece of fin ray was clipped from each parent and stored in 95% ethanol at -20°C. The parents carried out artificial induction of labor by injecting hormones, and 87 broodstock were all injected. The method of injecting hormones: one-time injection of chorionic gonadotropin (HCG), injection dose: 200IU / kg; luteinizing hormone releasing hormone analogue (LHRH- A2), injection dose: 3ug / kg. 87 broodstock produced a total of 10kg of fertilized eggs. Take 1.2kg of fertilized eggs and put them in a pond with an area of ​​4 mu for hatching. After 1 day, it is estimated that 1.12 million larvae are cultivated. After 60 days of cultivation, the average length of larvae is 10 cm. Enter 6m × ...

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Abstract

The invention discloses an oval pompanos trevally family paternity test method. For an embodiment for preparing mixed families through multiple parents, 11 pairs of micro-satellite primers which are high in genetic diversity and stable in amplification efficiency are screened out. The purpose of detecting the individual parent-child relationship of an oval pompanos trevally family is achieved in combination with capillary electrophoresis sequencing and software analysis. By means of the method, the oval pompanos trevally paternity test accuracy rate can reach over 90%.

Description

technical field [0001] The invention relates to the technical field of fish breeding and molecular markers, in particular to a method for using microsatellite molecular markers to identify offspring parents of polyculture families of pomfret ovata. Background technique [0002] Oval pomfret belongs to Perciformes (Perciformes), carangidae (Carangidae), pomfret subfamily (Trachinotinae), pomfret (Trachinotus), commonly known as golden pomfret, yellow wax pomfret, etc. It is widely distributed in tropical and subtropical waters and has the characteristics of fast growth, tender and delicious meat, and high nutritional value. It has become an important economic species for pond and cage culture in southern my country. [0003] In recent years, the market demand for oval pomfret has risen sharply, and it has become the largest species of marine fish cultured in southern China. At present, the annual output of golden pompano commercial fish in my country is more than 200,000 tons,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6848C12Q1/6888C12Q2600/156C12Q2549/119C12Q2563/107
Inventor 骆剑陈国华张国庆吴光灿王珺
Owner HAINAN UNIVERSITY
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