Method for screening megalobrama amblycephala group combinations with hybrid vigor

A combination of advantages and the technology of bream, applied in the directions of biochemical equipment and methods, microbial determination/inspection, climate change adaptation, etc., can solve problems such as decline of germplasm resources, destruction of natural resources, slowing down of growth rate, etc., and achieve results. Accurate and reliable, obvious hybrid vigor, and the effect of improving the effect

Inactive Publication Date: 2012-06-13
HUAZHONG AGRI UNIV
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Problems solved by technology

However, with the rapid development of the aquaculture industry in recent years, natural resources have been severely damaged, artificial inbreeding has intensified,

Method used

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  • Method for screening megalobrama amblycephala group combinations with hybrid vigor
  • Method for screening megalobrama amblycephala group combinations with hybrid vigor
  • Method for screening megalobrama amblycephala group combinations with hybrid vigor

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Embodiment 1

[0031] A method for screening group head bream with hybrid vigor, the steps are:

[0032] A. Collection of parents of wild group of bream and breeding in groups:

[0033] From February to May 2009, 100 parents of wild pomace bream were collected from Liangzi Lake and Silt Lake in Hubei Province and Poyang Lake in Jiangxi Province. They were raised in different ponds and fed with artificial compound feed (“Shengfeng” brand fish compound Feed), supplemented by an appropriate amount of green material (10Kg / mu per day). In June of that year, choose Liangzihu, which is healthy, larger and in better shape. mud lake and Poyang Lake A total of 92 parents were induced to give birth. Two injections were used to induce labor in bream, the first injection of luteinizing hormone-releasing hormone analogue (LRH-A 2 )1μg / Kg, the second injection is LRH-A 2 4μg / Kg + dioconone 5mg / Kg + chorionic gonadotropin 500IU / Kg (Note: the above oxytocic agents are all from Hangzhou Animal Phar...

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Abstract

The invention discloses a method for screening megalobrama amblycephala group combinations with hybrid vigor. The method comprises the following steps of: a, collecting wild parents of the megalobrama amblycephala in an original production area, and matching and breeding; b, culturing filial generations in the same pond in a mixed manner, and measuring relevant growth characteristics of the filial generations after 18 months; c, identifying the source of parents of the filial generations based on microsatellite markers; and d, based on a paternity test and growth characteristic data, analyzing the group combinations with the hybrid vigor, and finding that weights, lengths and heights of the filial generations in three combinations are obviously higher than that of other combinations. Different sources of wild parents are subjected to group combination breeding; a parent gene pool is enlarged; more gene types are collected; the hybrid vigor is sufficiently utilized; and the filial generations grow faster, thus, the megalobrama amblycephala selective breeding effect is more obvious.

Description

technical field [0001] The invention belongs to the field of fish breeding, and in particular relates to a method for screening group combinations of group head bream with hybridization advantage, which is suitable for the breeding research of the three wild groups of group head bream in Liangzi Lake, Silt Lake and Poyang Lake. technical background [0002] Fish is an important source of food for humans. my country is the country with the highest aquaculture output in the world. In addition to meeting domestic market demand, exporting is also one of the main purposes. However, with the rapid development of the aquaculture industry in recent years, natural resources have been severely damaged, artificial inbreeding has intensified, the germplasm resources of various fishes have been declining, and the growth rate has been slowing down, causing major economic losses to the aquaculture industry. . [0003] Group head bream (Megalobrama amblycephala Yih), belonging to Cyprinida...

Claims

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Application Information

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IPC IPC(8): A01K61/00C12Q1/68
CPCY02A40/81
Inventor 罗伟王卫民高泽霞曾聪邓伟
Owner HUAZHONG AGRI UNIV
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