Sebastes schlegeli microsatellite DNA (Deoxyribonucleic Acid) molecular marker

Abstract

The invention discloses a sebastes schlegeli microsatellite DNA (Deoxyribonucleic Acid) molecular marker. The molecular marker comprises construction of sebastes schlegeli microsatellite (CA) 16 and (GA) 16 enriched libraries and screening and sequencing of a microsatellite sequence-containing positive clone and determines 10 microsatellite markers SS101, SS102, SS103, SS104, SS105, SS106, SS107, SS108, SS109 and SS110 with rich polymorphism. The invention provides 10 sebastes schlegeli new microsatellite sites and a primer sequence and an amplification method for amplifying the 10 microsatellite sites; the molecular marker can be applied to population genetic structure analysis, paternity identification, molecular marker-assisted selection and the like of the sebastes schlegeli, and is a reliable and effective molecular marker.

Description

technical field [0001] The invention belongs to the technical field of DNA markers in molecular biology, and in particular relates to a microsatellite site and a primer of the genus chrysalis. Background technique [0002] Microsatellite DNA, also known as short tandem repeats (STRs), simple sequence repeats (SSRs), and simple sequence length polymorphism (SSLP), refers to the core sequence composed of 1 to 6 nucleotides in the genome. nucleotide sequence. According to the composition of repeating units, microsatellite DNA sequences are divided into three types: single type (pure), compound type (compound) and interrupted type (interrupted). E.g: [0003] Single type: AGAGAGAGAGAGAGAG; [0004] Composite type: AGAGAGAGAGACACAC; [0005] Intermittent type: AGAGAGTTAGAGAGTT. [0006] Microsatellite markers have the advantages of wide distribution, high polymorphism information capacity, co-dominance, Mendelian segregation, easy PCR amplification, and good reproducibility...

AI Technical Summary

Problems solved by technology

However, there are still very few microsatellite markers developed and officially published for P. xu's at present, and there are only 4 related documents: Yoshida et al. ) 10 Probes were used to perform hybridization screening on part of the genome library, and 6 pairs of microsatellite primers were developed; An et al. (An et al., Conservation genetics, 2009, 10, 1969) used biotin-labeled (CA) 12 A combination of probe hybridization and magnetic bead enrichment method developed 14 pairs of microsatellite primers; Bai et al. (Bai et al., Genetics and molecular research, 2011, 10, 2065) used biotin-labeled (GT) 13 A combination of probe hybridization and magnetic bead enrichment method developed 18 pairs of microsatellite primers; Yasuike et al. (Yasuike et al., Conservation genetics resources , 2012, 5, 577) used 454 sequencing technology to obtain some trinucleotides Microsatellite sequences with acid, tetranucleotide, and pentanucleotide repeats, and developed 17 pairs of polymorphic microsatellite primers

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