Polynary high-throughput genetic marking system and genetic analysis method for blue crabs
A genetic analysis method, the technology of Portunus trituratus, applied in the fields of biochemical equipment and methods, DNA/RNA fragments, microorganism determination/inspection, etc. and sustainability, clear genetic background, less burdensome effects
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[0048] 1) The multivariate high-throughput genetic marker system of Portunus trituberculatus is the mitochondrial sequence information system and the microsatellite molecular marker system, in which the microsatellite molecular marker is composed of system 1 with 7 SSR markers and system 2 with 6 SSR markers (see figure 1 );
[0049] System 1 consists of 7 SSR markers, and the 7 pairs of primers for amplifying the 7 SSR markers are
[0050] E13 (F: AAGGAGGAGGCTTTGATATG; R: GTCTTCCACTCCCTACTGT);
[0051] P04(F: GCCACTATCTTGCTGAGGTTGA; R: GCCATAGCACGAACACTTTTGA);
[0052] E20 (F: CCTCAGGGCAGCTCTGGAT; R: CCCGGACTAGGTGACAGGTT);
[0053] E24 (F: AACTATGGGTCCTGAGTATTA; R: CTCGGTTTCTGTCCACTT);
[0054] C13(F: CTGTCTGATGAGTGAGGCTAC; R: TGACCACGAGGAAAGGAG);
[0055] C31 (F: TATAAGGGCTTAAGTGTACG; R: GATCTAAACTCAAGGCAAAA);
[0056] H4(F: AATCACTTCACTACACCCTTTT; R: CTTGATGGGTGGCAGTCT);
[0057] System 2 consists of 6 SSR markers, and the 6 pairs of primers for amplifying the 6 SSR m...
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