Method for development and application of endangered rhododendron molle polymorphism SSR molecular marker

A molecular marker, sheep stalk technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problem of lack of SSR molecular markers of sheep sway polymorphism

Active Publication Date: 2016-06-01
JIANGXI NORMAL UNIV
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Problems solved by technology

Simple sequence repeat (SSR), which widely exists in eukaryotic genomes, has the advantages of co-dominance, high repeatability, and high abundance, and is an ideal tool for studying genetic diversity in populations. SSR molecular markers are still lacking

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  • Method for development and application of endangered rhododendron molle polymorphism SSR molecular marker
  • Method for development and application of endangered rhododendron molle polymorphism SSR molecular marker
  • Method for development and application of endangered rhododendron molle polymorphism SSR molecular marker

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Embodiment Construction

[0016] The following will be combined with figure 1 , 2 The beneficial effects of the present invention are described in detail, aiming at helping readers better understand the essence of the present invention, but not limiting the implementation and protection scope of the present invention.

[0017] 1. Acquisition of SSR molecular markers of A. chinensis: the improved 2×CTAB method was used to extract genomic DNA from the young leaves of A. spp. IlluminaHiSeq / MiSeq (IlluminaHiSeq TM 2500 / Miseq TM The sequencing platform performs simplified genome technology (RAD-seq). The RawData or RawReads after sequencing are quality-controlled and filtered, and clustered with cd-hit-est clustering software. Sequence-like sequencing data were assembled to obtain a genome sequence of 171.534Mb, including 498,252 contigs. The SSR search software was used to perform SSR detection and search on the contig sequence, and finally 11,687 SSR molecular markers were developed from 11,961 microsa...

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Abstract

The invention discloses a method for development and application of endangered rhododendron molle polymorphism SSR molecular markers. The method comprises the following steps: developing by using a simplified genome sequencing technique so as to obtain 11687 pairs of rhododendron molle SSR molecular markers, and identifying so as to obtain 16 pairs of polymorphism SSR molecular markers, wherein the polymorphism SSR molecular markers can be used for detecting SSR locus information of rhododendron molle genomes; the effective number of alleles of the rhododendron molle genomes is 3-15; the heterozygosity (He) of the rhododendron molle genomes is 0.489-0.908. The invention provides a series of SSR primers which can be applied to study of population genetics, phylogenetics, conservation biologics and the like, the polymorphism SSR molecular markers can be used for evaluating and analyzing genetic diversity and genetic structures of endangered rhododendron molle, and scientific basis and reference can be provided for protection of the specie.

Description

technical field [0001] The invention relates to a method for the development and application of polymorphic SSR molecular markers of endangered sheep roe deer. Background technique [0002] Rhododendronmolle G.Don, also known as Rhododendron molle G.Don, is the only native species in the subgenus Rhododendronmolle in China, and has high ornamental value and medicinal value. The organs of the whole plant of the goat roe deer contain a variety of active ingredients, and its extracts can treat rheumatoid, febrile malaria, chronic glomerulonephritis and high blood pressure, etc., and also have a very good killing effect on schistosomes and various pests. [0003] Before the 1980s, sheep's roe spread throughout central and southern China, and a small amount was also distributed in southwest China. However, due to people's indiscriminate digging and habitat destruction, the reproductive link of sheep's roe has been seriously affected. The areas and populations of sheep's roe in t...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6869C12Q1/6895C12Q2600/156C12Q2525/151
Inventor 罗向东程马龙戴亮芳田欢谢建坤
Owner JIANGXI NORMAL UNIV
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