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Microsatellite primer used for analyzing diversity of pachycrepoideus vindemmiae, purpose and analysis method

A technology for diversity analysis and fly pupae, which is applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., to achieve the effect of stable PCR amplification products and good polymorphism

Active Publication Date: 2014-08-13
ANHUI NORMAL UNIV
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  • Application Information

AI Technical Summary

Problems solved by technology

Up to now, there is no set of microsatellite primers in the world to analyze the genetic structure of the fly chrysalis

Method used

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  • Microsatellite primer used for analyzing diversity of pachycrepoideus vindemmiae, purpose and analysis method
  • Microsatellite primer used for analyzing diversity of pachycrepoideus vindemmiae, purpose and analysis method
  • Microsatellite primer used for analyzing diversity of pachycrepoideus vindemmiae, purpose and analysis method

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Experimental program
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Effect test

Embodiment 1

[0057] 1. Primer synthesis: Constructed the genome library of the chrysalis chrysalis, screened out polymorphic microsatellite primers, and sent them to Shanghai Sangong to synthesize 10 pairs of primers. The sequence is as follows:

[0058]

[0059]

[0060] 2. Genomic DNA extraction: Use the animal genomic DNA extraction kit from Shanghai Sangon Bioengineering Technology Service Co., Ltd. for DNA extraction:

[0061] 1) Take out 60 individuals from the sample, soak them in double distilled water and wash them.

[0062] 2) Add it to a 1.5ml centrifuge tube, grind it into powder, add 400μL Buffer Digestion, shake and mix

[0063] 3) Water bath at 65°C for 1 hour until the cells are completely lysed.

[0064] 4) Add 200 μL of Buffer PA, mix thoroughly by inversion, and place in a -20°C refrigerator for 5 minutes.

[0065] 5) Centrifuge at 10,000 rpm for 5 min at room temperature, and transfer the supernatant (500-550 μL) to a new 1.5 ml centrifuge tube.

[0066] 6) Add...

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Abstract

The invention relates to a microsatellite primer used for analyzing diversity of pachycrepoideus vindemmiae, a purpose and an analysis method. The analysis method comprises the following steps: 1)extracting genome DNA; 2)detecting a TP-M13-SSR molecular marker; and 3)analyzing a genetic structure. DNA is extracted from a sample, the site of a microsatellite is screened, so that the primer is designed. The primer has the advantages of stable PCR amplification product and good polymorphism, and can be used for analysis of diversity and a population genetic structure.

Description

technical field [0001] The invention relates to the field of insect evolution, in particular to a microsatellite primer for analyzing the diversity of the chrysalis chrysalis and its application and analysis method, which can be used for the analysis of the genetic diversity of the chrysalis chrysalis population. Background technique [0002] Parasitic wasps are important natural enemies of pests and play an important regulatory role in controlling the population density of pests. In production practice, applying and properly maintaining the density of parasitoids can control the pest population density within a certain threshold for a long time. By giving full play to the advantages of biological control that does not pollute the environment, does not kill natural enemies, and has continuous disaster control effects, it can achieve the goals of protecting the ecological environment, protecting biodiversity, and continuously controlling pests. Pachycrepoideus vindemmiae (Ro...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 胡好远陈伟方磊刘佳璐贺张
Owner ANHUI NORMAL UNIV
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