Litopenaeus vannamei osmotic regulation related functional gene EST-SSR markers and specific primers and detection method thereof

A technology of osmotic pressure regulation and related functions, applied in the field of molecular markers, which can solve the problems of less EST-SSR marker development and no EST-SSR marker development yet.
CN105969873AActive Publication Date: 2016-09-28SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Publication Date
2016-09-28

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Abstract

The invention discloses litopenaeus vannamei osmotic regulation related functional gene EST-SSR markers and specific primers and a detection method thereof. Litopenaeus vannamei genome DNA serves as a template, the specific primers of the litopenaeus vannamei osmotic regulation related functional gene EST-SSR markers are used for conducting PCR amplification on microsatellite locus sequences, and then typing is conducted on PCR amplification products through a sequenator. The specific primers and detection method of the litopenaeus vannamei osmotic regulation related functional gene EST-SSR markers can be used for population genetic structure analysis and molecular marker assisted breeding of litopenaeus vannamei and particularly have an important application value in breeding of good salt-tolerant varieties of the litopenaeus vannamei.
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Description

Technical field:

[0001] The invention belongs to the technical field of molecular markers, and in particular relates to EST-SSR markers of functional genes related to osmotic pressure regulation of Litopenaeus vannamei, specific primers and detection methods thereof. Background technique:

[0002] Litopenaeus vannamei, commonly known as Penaeus vannamei, is native to South America and is distributed from the southwestern coast of Mexico to the Pacific coast in western Peru. It is the largest farmed prawn in the world. In my country, Litopenaeus vannamei aquaculture areas are spread all over the country's coastal marine aquaculture areas and inland freshwater aquaculture areas, and it is a pillar industry of aquaculture in my country. Litopenaeus vannamei introduced and bred in my country are all artificially bred populations, which are prone to genetic decline. Therefore, the selection and breeding of improved species is a major issue related to the success or failure of Li...

Claims

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