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Genetic diversity analysis method for inbreeding male giant freshwater prawn

A technology of genetic diversity and Macrobrachium rosenbergii is applied in the field of genetic diversity analysis of inbreeding male Macrobrachium rosenbergii. The effect of improving the quality of seedlings

Inactive Publication Date: 2018-10-12
GUANGXI ACADEMY OF FISHERY SCI
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Problems solved by technology

At present, the long-term inbreeding of Macrobrachium rosenbergii autonomously or artificially leads to the degradation of germplasm, and it is urgent to identify and differentiate them
Coupled with the uncontrollable male morphological inheritance of Macrobrachium rosenbergii, the accuracy of the genetic difference evaluation of the male parents is insufficient, which seriously affects the breeding of improved species. Therefore, microsatellite marker technology is used to find out the individual growth genetic diversity of inbreeding morphological male shrimp help to solve this real problem
[0004] At present, scholars at home and abroad have used microsatellite molecular marker technology to study the genetic diversity of Macrobrachium rosenbergii, but they mainly study the genetic diversity of different populations, and little is known about the same-sex genetic information obtained by natural or artificial inbreeding
Using 6 microsatellite loci to study the genetic diversity of wild Macrobrachium rosenbergii species, it was found that the average number of alleles per locus was highly diverse, however, only the number of alleles at each microsatellite marker locus was obtained The genetic diversity of this species is limited

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  • Genetic diversity analysis method for inbreeding male giant freshwater prawn
  • Genetic diversity analysis method for inbreeding male giant freshwater prawn
  • Genetic diversity analysis method for inbreeding male giant freshwater prawn

Examples

Experimental program
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Effect test

Embodiment 1

[0035] 1.1 Sample collection

[0036] Inbreeding adult Macrobrachium rosenbergii populations in Guangxi, 200 tails, provided by the National (Nanning, Guangxi) Macrobrachium rosenbergii Breeding Center, cultured for 4 months in the laboratory breeding pond (5×5×1.8m) of the Fine Breeding Center, based on color, Behavior, growth characteristics and serrated claws are divided into three different morphological types of male shrimps: blue long arm type (BCM), orange long arm type (OCM) and small body type (SM). There were 50 tails for each of the 3 morphotypes, and a total of 150 tails were collected. The body weights of BCM, OCM, and SM were 14.48±1.05g, 10.04±1.15g, and 5.86±0.66g, respectively.

[0037] 1.2 DNA extraction

[0038] The genomic DNA of the abdominal muscle tissue of Macrobrachium rosenbergii was extracted according to the steps of the Wizard Genomic DNA Purification Kit (purchased from Promega, USA), and the absorbance (OD) of the extract at 260nm and 280nm was ...

Embodiment 2

[0049] [Example 2] Results and Analysis

[0050] 2.1 Population genetic diversity

[0051] It can be seen from Table 2 that, except for SM locus 5, the genetic information of other loci is polymorphic, and this locus should be removed in all statistical analysis. The male shrimp populations of the three morphotypes showed high genetic differences. The allele numbers of BCM, OCM, and SM were 2.885, 3.762, and 2.627, respectively, and the observed heterozygosity were 0.5561, 0.474, and 0.475, respectively. The exact deviation value (P) of the Hardy-Weinberg equilibrium law was estimated using the Markov chain method and subsequently corrected by the Bonferroni multiple hypothesis experiment. The results showed that most loci were significantly shifted.

[0052] Table 2 Genetic diversity of satellite loci in male Macrobrachium rosenbergii of three morphotypes

[0053]

[0054] G: gene copy number, A: allele number, H o : observed heterozygosity, H e : Expected heterozygos...

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Abstract

The invention discloses a genetic diversity analysis method for a inbreeding male giant freshwater prawn, which comprises the following steps of: step 1, acquiring inbreeding giant freshwater prawn samples with different traits, and extracting the DNA; step 2, selecting a microsatellite marker combination and designing a specific PCR primer set for amplification; step 3, evaluating the number of PCR products and observing the genotype and size of the alleles; step 4, recording the allele number A, the allele duplication number G, the observed heterozygosity Ho, the desired heterozygosity He, and the accuracy deviation value P based on the Hardy-Weinberg equilibrium law of each prawn, and analyzing the difference of the microsatellite sites; calculating the gene flow Nm according to the frequency parameters of the population allele; gene spacing is determined by using the gene Nei coefficient method. According to the invention, based on the microsatellite analysis method, the method provides a genetic diversity analysis method for a inbreeding male giant freshwater prawn, which is very suitable for researching the genetic structure of different near-parent male prawn groups, and provides a high-quality male parent for the giant freshwater prawns.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a method for analyzing genetic diversity of inbreeding male Macrobrachium rosenbergii. Background technique [0002] Macrobrachium rosenbergii (Macrobrachium rosenbergii, De Man 1879) is a large freshwater prawn, which has gradually become an important aquaculture species in my country, Southeast Asia and even the world because of its wide tolerance to water quality changes, easy breeding, tender meat, and high commercial value. . At present, the increasing production of Macrobrachium rosenbergii is still difficult to meet the increasing consumption and market demand, so it is necessary to further break through the factors that limit the increase in the production of Macrobrachium rosenbergii to greatly increase the output. Because male shrimp grows faster than female shrimp, matures larger, and has a higher breeding output [1], it is mainly to solve the limiting fact...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6888C12Q2600/156C12Q2525/151
Inventor 马华威吕敏黄光华杨慧赞甘晖卢小花杨琼王瑞曾兰杨彦豪阮志德卢天和
Owner GUANGXI ACADEMY OF FISHERY SCI
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