Microsatellite fluorescence multi-PCR method for testing paternity of culter alburnus basilewsky

Abstract

The invention relates to a microsatellite fluorescence multi-PCR method for testing the paternity of culter alburnus basilewsky. The method comprises the following steps: firstly, 16 pairs of SSR primers are screened out, 12 pairs of SSR primers are chosen through optimal combination to form 3 multi-PCR groups; 4 pairs of SSR specific primer pairs belonging to the same group but having different concentrations are then added into a PCR reaction system, different reaction systems are used to amplify different areas of one same DNA template to form a plurality of target fragments; the target fragments are sent to a biological company for typing on a sequencer after the sampling inspection through electrophoresis, a genotype on a corresponding lotus of a sample is acquired; and finally the genotype is used to analyze the parentage of the parent and the progeny. A related microsatellite marker amplification system saves the DNA sample, is high in experiment efficiency, and can be popularized on the paternity of culter alburnus basilewsky as well as evaluation of genetic variation of population and hereditary constitution.

Description

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AI Technical Summary

Benefits of technology

This technology allows researchers to detect specific areas on chromosomal DNA called microspots (DNA fragments) that may indicate different traits or diseases like cancer. It simplifies this process by saving only necessary materials while still being able to accurately identify these regions within an individual's genome.

Problems solved by technology

This patented describes various techniques related to studying microscopic organisms such as bacteria or plants called cullstrahols during their life cycle from seafoods through turtles on land. These methods require many steps like extracting nucleic acid material from them, preparation of reagents, analysis by sequencing, etc., resulting in significant expenses associated with each step. Additionally, these conventional approaches involve analyzing numerous small sections of DNA obtained from several genera, making it difficult to identify specific sequences within those segments without consuming too much data.

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