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Production method of organic potassium fertilizer strain agent and biologic organic fertilizer

A technology of inoculum and potash fertilizer, applied in the production field of organic potash fertilizer inoculum and bio-organic fertilizer, can solve the problems of salt tolerance and growth inhibition, etc.

Inactive Publication Date: 2009-12-23
肖君
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the salt tolerance of the bacterium is poor, and its growth is significantly inhibited in medium with higher salt concentration.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. A kind of organic potassium fertilizer inoculum, its preparation method is as follows:

[0042] (1) After activating Bacillus mucilaginosus on a nitrogen-free medium, transfer it to a liquid medium that inhibits capsule growth and culture it to the logarithmic growth phase, and collect the bacteria by centrifugation; use diluted seawater Or suspend the bacteria in potassium-containing brine and dilute the bacteria concentration to 10 6 ~10 8 cells / ml, to obtain the bacterial cell suspension, the bacterial cell suspension is subjected to mutagenesis by radiation mutagenesis or chemical mutagenesis, and the mutated bacterial cell suspension is centrifuged to obtain the mutagenic cell; Screening and re-screening are carried out to obtain salt-tolerant bacterial strains; the method is as follows:

[0043] 1) Use the screening medium to suspend the mutagenized cells, and adjust the concentration of the mutagenized cells to 10 5 ~10 6 cells / ml, shake well, and...

Embodiment 2

[0047] Example 2. A kind of organic potassium fertilizer inoculum, its preparation method is as follows:

[0048] (1) After activating Bacillus mucilaginosus on a nitrogen-free medium, transfer it to a liquid medium that inhibits capsule growth and culture it to the logarithmic growth phase, and collect the bacteria by centrifugation; use diluted seawater Or suspend the bacteria in potassium-containing brine and dilute the bacteria concentration to 10 6 ~10 8 cells / ml, to obtain the bacterial cell suspension, the bacterial cell suspension is subjected to mutagenesis by radiation mutagenesis or chemical mutagenesis, and the mutated bacterial cell suspension is centrifuged to obtain the mutagenic cell; Screening and re-screening are carried out to obtain salt-tolerant bacterial strains; the method is as follows:

[0049] 1) Use the screening medium to suspend the mutagenized cells, and adjust the concentration of the mutagenized cells to 10 5 ~10 6 cells / ml, shake well, and...

Embodiment 3

[0053] Example 3. A kind of organic potassium fertilizer inoculum, its preparation method is as follows:

[0054] (1) After activating Bacillus mucilaginosus on a nitrogen-free medium, transfer it to a liquid medium that inhibits capsule growth and culture it to the logarithmic growth phase, and collect the bacteria by centrifugation; use diluted seawater Or suspend the bacteria in potassium-containing brine and dilute the bacteria concentration to 10 6 ~10 8 cells / ml, to obtain the bacterial cell suspension, the bacterial cell suspension is subjected to mutagenesis by radiation mutagenesis or chemical mutagenesis, and the mutated bacterial cell suspension is centrifuged to obtain the mutagenic cell; Screening and re-screening are carried out to obtain salt-tolerant bacterial strains; the method is as follows:

[0055] 1) Use the screening medium to suspend the mutagenized cells, and adjust the concentration of the mutagenized cells to 10 5 ~10 6 cells / ml, shake well, and...

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Abstract

The invention relates to an organic potassium fertilizer microbial agent. The organic potassium fertilizer microbial agent is characterized by being prepared by the following steps: after salt-tolerance colloid bacilli are activated on a nitrogen-free culture medium, the salt-tolerance colloid bacilli are transferred into a liquid culture medium and cultured, thalluses are collected by centrifugation, then kieselguhr, active carbon or plant ash with equivalent quantity is added as an adsorbent, and the mixture is dried at low temperature to obtain the organic potassium fertilizer strain agent. The invention also relates to a production method of biologic organic fertilizer. The organic potassium fertilizer microbial agent is prepared by combining a culture medium prepared by adopting seawater or bittern and the salt-tolerance colloid bacilli which are obtained by chemomorphosis or radialization mutagenesis and directed mutagenesis, can adapt to the seawater with a certain concentration and contain potassium bittern, the potassium content of the organic potassium fertilizer microbial agent is higher, and the organic potassium fertilizer microbial agent can be used as the biologic organic fertilizer, can be directly applied to common soil and salt alkali soil and can promote the absorption of plants to the soil and potassium in the microbial agent. The organic potassium fertilizer microbial agent also can be used as the organic fertilizer microbial agent and can produce organic potassium fertilizer and composite potassium fertilizer.

Description

technical field [0001] The invention relates to an organic fertilizer seed agent, in particular to an organic potassium fertilizer seed agent; the invention also relates to a production method of biological organic fertilizer. Background technique [0002] Potassium fertilizer is one of the three major fertilizers in agriculture, and it has obvious yield-increasing effect on most crops. The raw material of potash fertilizer is potassium salt, which is the general term for potassium-containing minerals. According to its solubility, it can be divided into soluble potassium salt minerals and insoluble potassium-containing aluminosilicate minerals. The former is a utilizable mineral resource formed by the accumulation of soluble potassium-containing salt minerals in nature; it includes soluble solid potassium salt deposits (such as potassium halite, carnallite, polyhalite, etc.) formed by evaporation, concentration and deposition of potassium-containing water bodies. etc.) and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N15/01C12Q1/04C05F11/08C12R1/07
Inventor 肖君程昌林温扶辉
Owner 肖君
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