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Gene sequence and application thereof

A gene sequence and sequence technology, applied in the application field of the gene sequence, can solve problems such as no discovery, and achieve the effects of high content of malt-oligosaccharide, high nutrition and health care efficacy

Inactive Publication Date: 2015-09-16
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In addition, it has not been found that the rice can produce a giant embryo phenotype, and the vitamin E content and γ-aminobutyric acid content in rice are increased compared with non-megaembryo rice. At the same time, the glutathione content, maltooligosaccharide content macroembryonic allele

Method used

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  • Gene sequence and application thereof
  • Gene sequence and application thereof
  • Gene sequence and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Choose three types of rice:

[0046] 1) Rice "Nipponbare" containing Os07g0603700 megaembryo gene (no mutation in coding strand and promoter);

[0047] 2) There is no mutation in the coding strand of the Os07g0603700 giant embryo gene, but an adenine deoxynucleotide is inserted between the -777 and -778 deoxynucleotide upstream of the first deoxynucleotide of the first codon of the coding strand The giant embryo gene, that is, the rice "Chao 2-10" with only the promoter of the giant embryo gene mutated;

[0048] 3) An adenine deoxynucleotide was inserted between the -777 and -778 deoxynucleotide upstream of the first deoxynucleotide of the first codon of the coding strand of the Os07g0603700 giant embryo gene, and at the same time, the first codon of the coding strand At +125 downstream of the first deoxynucleotide of the first codon, the macroembryonic gene that is mutated from guanine deoxynucleotide to adenine deoxynucleotide, that is, the macroembryonic gene with m...

Embodiment 2

[0071] Embodiment 2 will mainly describe and utilize giant embryo gene ge of the present invention c The invention relates to a method for cultivating various new rice varieties with giant embryo phenotypes and multiple important nutrients contained in brown rice. The giant embryo genotype in common embryo rice is represented by GG, which contains ge c The giant embryo genotype in the giant embryo rice "Shangshida No. 5" rice is represented by gg. First, the common embryo rice (GG) was crossed with the giant embryo rice "Shangshida No. 5" (gg) in Example 1 to obtain F1 seeds and planted to obtain F1 plants. The giant embryo genotype of the F1 plants was Gg. F1 plants were selfed to obtain F2 seeds, which were planted to obtain F2 plants. According to the genetic characteristics of the megaembryo gene, there are three types of megaembryo genotypes in F2 plants: GG, Gg and gg.

[0072] The leaves of F2 plants were taken, the total DNA was extracted, the megaembryo gene was am...

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Abstract

The invention provides a new gene sequence and an application thereof. The gene sequence is characterized in that am adenine deoxynucleotide is inserted between the upstream-777 and the upstream-778 of the first deoxynucleotide of the first codon of a giant embryo allele coding strand corresponding to normal embryo rice, and guanine deoxynucleotide in the downstream+125 of the first deoxynucleotide of the first codon of the coding strand mutates to adenine deoxynucleotide. The GABA content, the vitamin E content, the glutathione content and the malto-oligosaccharide content of homozygous brown rice with the gene sequence are obviously higher than those of brown rice without the gene sequence. The gene sequence is transferred to normal embryo rice to obtain homozygous giant embryo progeny, so a new species of giant embryo rice with giant embryo phenotype and with obviously improved content of various nutrients in brown rice can be obtained.

Description

technical field [0001] The present invention relates to a gene sequence, in particular to a gene sequence capable of enabling plants to exhibit the characteristics of giant embryos and making rice brown rice contain various important nutrients such as glutathione and maltooligosaccharides, and the application of the gene sequence . Background technique [0002] Rice is one of the most important food crops for human beings, and its rice is the staple food of Chinese people. If rice is rich in nutrients that have important health care functions for the human body, such as γ-aminobutyric acid, vitamin E, glutathione, maltooligosaccharides, etc., eating such rice can not only have health care functions for the human body, Moreover, such rice can also be used as a raw material for extracting these important nutrients. [0003] Giant embryo rice is named for its brown rice having a larger embryo, which is usually 2-4 times larger than ordinary brown rice. [0004] (Maeda et al....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29A01H1/02
Inventor 李建粤张鑫谢米雪时亚琼张涛平宝哲王杰
Owner SHANGHAI NORMAL UNIVERSITY
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