Device for cell culture

Abstract

A device for cell culture capable of automatically performing operations for cell culture over several days to several months while minimizing the risk of contamination. A new medicine can be supplied to an incubator means by using a medicine supply means or unnecessary wastewater can be discharged from the incubator means by using a wastewater discharge means without taking out the incubator means disposed in heat insulation box means from a heat insulation box, and the state of the cell culture can be observed with the incubator means formed in the heat insulation box means. Accordingly, the outside air does not enter directly into the incubator means during culturing, and the risk of contamination is completely eliminated. As a result, the culturing operations can be automatically performed over a long period.

Description

technical field [0001] The present invention relates to a cell culture device for culturing cells, and particularly relates to a cell culture device capable of automatically performing operations involving culturing for several days to several months. Background technique [0002] In cell culture, cumbersome subculture processes such as exchanging the medium in the incubator and reseeding to make the cell density appropriate are performed manually. Usually, these operations are performed very carefully in a relatively clean atmosphere by suppressing the concentration of floating fine particles in the atmosphere by using the clean environment generation technology established in the semiconductor manufacturing field in order to avoid pollution and the like. However, even in this purified atmosphere, it is not sufficient to avoid contamination. When cells are cultured in a circular dish commonly used as an incubator, the following complicated and difficult operations need to b...

AI Technical Summary

Problems solved by technology

For example, in USP5,985,653 (Patent Document 1), a culture device with a swing mechanism is disclosed for uniform seeding, but because it is not completely closed, there is still a risk of contamination

[0007] As mentioned above, cell culture devices have major problems in terms of mass production and safety compatibility

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