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Device and method for noninvasive continuous monitoring of quantity or concentration of dynamic cells

A non-invasive, cell number technology for bioengineering applications

Inactive Publication Date: 2011-09-28
SHANGHAI KUNJU TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Second, only the initial inoculation and final harvested cell number or cell concentration data of the cell growth process can be obtained, and no data during the cell growth process can be obtained

Method used

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  • Device and method for noninvasive continuous monitoring of quantity or concentration of dynamic cells
  • Device and method for noninvasive continuous monitoring of quantity or concentration of dynamic cells
  • Device and method for noninvasive continuous monitoring of quantity or concentration of dynamic cells

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Experimental program
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Effect test

Embodiment Construction

[0014] The core of the present invention is that,

[0015] Under normal circumstances, the consumption of a certain nutrient, or the metabolism of a certain metabolite, or the production of a certain cell's own product by a unit amount of a specific cell in a steady state in a short unit of time The amount, that is, the specific consumption rate or specific metabolic rate or specific production rate of cells is basically constant; that is, the total number of viable cells or the concentration of viable cells in a specific cell culture (system) is the same as that of the cells at that time. The consumption rate or metabolic rate or production rate of nutrients or metabolites or products are in a certain proportional relationship. Thus, by detecting changes in the above-mentioned substances (which may be more than one target substance) due to their consumption, metabolism or production by cells, the number of viable cells or the number of viable cells in the entire cell culture ...

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Abstract

The invention relates to a device and method for noninvasive continuous monitoring of quantity or concentration of dynamic cells, in particular to a device and method for noninvasive continuous on-line or off-line monitoring of quantity or concentration of dynamic cells in adherent cell cultures (usually as stem cell cultures or other therapeutic cell cultures) or cell and tissue cultures. The device comprises: a cell culture medium supply system, one / one type of or multiple / multiple types of upstream biomedical indicator detection head(s) or connector(s), a cell culture , one / one type of or multiple / multiple types of downstream biomedical indicator detection head(s) or connector(s), one or multiple speed controllable sterile driving unit(s) of fluid, a waste or harvested liquor system, liquid conveying pipeline systems that are driven and controlled in speed by the speed controllable sterile driving unit(s) of fluid for connecting the above components in order. And the upstream and downstream biomedical indicator detection head(s) or connector(s) are connected to a monitoring and controlling system and / or a computer so as to obtain, process and monitor data, and furthermore provide feedbacks for the whole cell culture system through a signal feedback system for realizing control.

Description

technical field [0001] The invention relates to a device and method for non-invasive continuous monitoring of dynamic cell quantity or concentration in the field of bioengineering, in particular to a method for culturing adherent cells (such as stem cells or other therapeutic cells in general) or for culturing A device and method for non-invasive continuous on-line or off-line monitoring of the number or concentration of dynamically growing cells for cell tissue culture. Background technique [0002] Usually, the in vitro culture and growth of stem cells or other therapeutic cells or cell tissues are adherent or fixed growth, which is usually carried out statically in a carbon dioxide cell incubator with a plate or a T-flask or a cell tissue culture device. or sessile culture. During the culture process, the cells are closely adhered to the inner wall of the plate or T-flask to grow or the cells are in the form of cell tissue, and the cells cannot freely dissociate. Theref...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/36C12M1/34C12Q3/00C12Q1/02
Inventor 齐瀚实郑琛
Owner SHANGHAI KUNJU TECH DEV
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