SNP marker related to auxiliary diagnosis of lung cancer of non-smoking women and applications thereof

An auxiliary diagnosis, non-smoking technology, applied in the field of SNP markers

Active Publication Date: 2015-01-21
江苏同凯兆丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no report on the application of SNP in the diagnosis of lung cancer in non-smoking women. If the SNPs that are susceptible to lung cancer in non-smoking women can be screened out as biomarkers, and the corresponding diagnostic kits c

Method used

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  • SNP marker related to auxiliary diagnosis of lung cancer of non-smoking women and applications thereof
  • SNP marker related to auxiliary diagnosis of lung cancer of non-smoking women and applications thereof
  • SNP marker related to auxiliary diagnosis of lung cancer of non-smoking women and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Example 1 The collection of samples and the arrangement of sample data

[0087] The inventor collected a large number of blood samples from non-smoking female lung cancer patients from April 2005 to December 2011 from the First Affiliated Hospital of Nanjing Medical University and Jiangsu Cancer Hospital. After sorting out the sample data, the inventor selected 1822 experimental samples of genome-wide microarray scanning and single SNP Sequenom MassARRAY genotyping of samples meeting the following criteria:

[0088] 1. Non-smoking female lung cancer patients diagnosed by histopathology;

[0089] 2. Non-smoking female healthy controls matched with the age of the case;

[0090] The demographic data and clinical data of these samples were collected systematically.

Embodiment 2

[0091] Example 2 Whole Genome Scanning of SNPs in Peripheral Blood DNA

[0092] Among the above-mentioned eligible 228 non-smoking female lung cancer patients and 568 non-smoking female healthy controls, the two groups were age-matched. The two groups of people were detected by Affymetrix6.0 chip to obtain relevant results. The specific steps are:

[0093] 1. Add hemolysis reagent (i.e. lysate, 40 parts) to the peripheral blood stored in the 2ml cryopreservation tube. Dilute the TrisHcl solution to 2000ml, the same below), turn it upside down and mix it completely.

[0094] 2. Removal of red blood cells: Fill the 5ml centrifuge tube to 4ml with hemolysis reagent, mix by inverting, centrifuge at 4000rpm for 10 minutes, and discard the supernatant. Add 4ml of hemolysis reagent to the precipitate, invert and wash again, centrifuge at 4000rpm for 10 minutes, and discard the supernatant.

[0095] 3. Extract DNA: Add 1ml extract solution to the precipitate (each 300ml contains 1...

Embodiment 3

[0105] Example 3 Sequenom MassARRAY genotyping of a single SNP

[0106] The SNPs found to be associated with the incidence of lung cancer in non-smoking women were detected by the above genome-wide scan on another 502 non-smoking female lung cancer cases and 524 non-smoking female healthy controls on the Sequenom MassARRAY genotyping platform. The specific steps are as follows:

[0107] 1. Add the hemolysis reagent to the peripheral blood stored in the 2ml cryopreservation tube, mix it upside down and transfer it completely.

[0108] 2. Removal of red blood cells: Fill the 5ml centrifuge tube to 4ml with hemolysis reagent, mix by inverting, centrifuge at 4000rpm for 10 minutes, and discard the supernatant. Add 4ml of hemolysis reagent to the precipitate, invert and wash again, centrifuge at 4000rpm for 10 minutes, and discard the supernatant.

[0109] 3. Extract DNA: Add 1ml of extract solution and 8μl of proteinase K to the precipitate, shake and mix well on the shaker, and ...

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Abstract

The invention belongs to the medical field of genetic engineering and tumors, and discloses an SNP marker related to auxiliary diagnosis of lung cancer of non-smoking women and applications thereof. The marker is a combination of 25 SNPs such as rs10051970, rs10087140, rs10483845, rs11233751, rs11643408, rs11732247, rs1255485, rs12612657, rs11643408, rs11732247, rs16935064, rs12612657, rs1535271, rs166402, rs16935064, rs12612657, rs1535271, rs166042, rs16935064, 17040797, rs17073021, rs17623941, rs17670695, rs2550911, rs40395, rs4454390 and rs4640379. The marker can be used for preparing a kit for the auxiliary diagnosis of lung cancer of non-smoking women, and applications thereof.

Description

field of invention [0001] The invention belongs to the fields of genetic engineering and tumor medicine, and relates to SNP markers related to auxiliary diagnosis of lung cancer in non-smoking women and applications thereof. Background technique [0002] Lung cancer is one of the most common malignant tumors that cause human death worldwide, and its morbidity and mortality are increasing rapidly, especially among women. Epidemiological surveys in recent years have shown that the incidence of lung cancer in women has increased more than that in men. According to the International Agency for Research on Cancer (IARC), among women, there were 583,000 new cases of lung cancer and 491,000 deaths in 2012, ranking fourth in cancer incidence and second in mortality worldwide. Due to environmental pollution, changes in lifestyle, and long-term exposure to environmental tobacco smoke and kitchen fumes, etc., the incidence of lung cancer among Chinese women, especially housewives, has ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 沈洪兵胡志斌靳光付马红霞戴俊程朱猛
Owner 江苏同凯兆丰生物科技有限公司
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