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Cancer-testis genetic marker related to primary lung cancer auxiliary diagnosis and application thereof

An auxiliary diagnosis and marker technology, applied in the fields of genetic engineering and tumor medicine

Active Publication Date: 2018-01-09
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the application of the SNP on the CT gene to the diagnosis of primary lung cancer. If the primary lung cancer susceptibility SNP on the CT gene can be screened as a biomarker and a corresponding diagnostic kit can be developed, It will definitely be a powerful impetus to the diagnosis status of primary lung cancer in my country, and it will also open up new ways for drug screening, drug efficacy evaluation and targeted therapy

Method used

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  • Cancer-testis genetic marker related to primary lung cancer auxiliary diagnosis and application thereof
  • Cancer-testis genetic marker related to primary lung cancer auxiliary diagnosis and application thereof
  • Cancer-testis genetic marker related to primary lung cancer auxiliary diagnosis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] The collection of embodiment 1 sample and the arrangement of sample data

[0069] The inventor collected a large number of primary lung cancer blood samples from the Affiliated Cancer Hospital of Nanjing Medical University and the First Affiliated Hospital of Nanjing Medical University from April 2005 to January 2011. After sorting out the sample data, the inventor selected 5,408 samples from Whole Genome Microarray scans and individual Sequenom MassARRAY genotyped experimental samples meeting the following criteria:

[0070] 1. Selection of research samples

[0071] (1) Clinically diagnosed as primary lung cancer;

[0072] (2) Healthy controls matched with the age and gender of the case;

[0073] The demographic data and clinical data of these samples were collected systematically.

Embodiment 2

[0074] Whole Genome Scanning of SNP in Example 2 Peripheral Blood DNA

[0075] Among the 2,331 primary lung cancer patients and 3,077 healthy controls who met the above criteria, the two groups were matched in age and gender. The two groups of people were detected by Affymetrix 6.0 chip to obtain relevant results. The specific steps are:

[0076] 1. Add hemolysis reagent (i.e. lysate, 40 parts) to the leukocytes stored in the 2ml cryopreservation tube. The volume of the solution was adjusted to 2000ml, the same below), and it was completely transferred after inverting and mixing.

[0077] 2. Removal of red blood cells: Fill the 5ml centrifuge tube to 4ml with hemolysis reagent, mix by inverting, centrifuge at 4000rpm for 10 minutes, and discard the supernatant. Add 4ml of hemolysis reagent to the precipitate, invert and wash once again, centrifuge at 4000rpm for 10 minutes, and discard the supernatant.

[0078] 3. Extract DNA: add 1ml extract solution to the precipitate (e...

Embodiment 3

[0087] Example 3 Sequenom MassARRAY genotyping of a single SNP

[0088] The SNPs found to be associated with the onset of primary lung cancer in the above genome-wide scan were detected in another 1,500 cases of primary lung cancer and 3,000 healthy controls. The specific steps are as follows:

[0089] 1. Add the hemolysis reagent to the leukocytes stored in the 2ml cryopreservation tube, mix it upside down and transfer it completely.

[0090] 2. Removal of red blood cells: Fill the 5ml centrifuge tube to 4ml with hemolysis reagent, mix by inverting, centrifuge at 4000rpm for 10 minutes, and discard the supernatant. Add 4ml of hemolysis reagent to the precipitate, invert and wash once again, centrifuge at 4000rpm for 10 minutes, and discard the supernatant.

[0091] 3. Extract DNA: Add 1ml of extract solution and 8μl of proteinase K to the precipitate, shake and mix well on a shaker, and place in a water bath at 37°C overnight.

[0092] 4. Remove protein: add 1ml of saturate...

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Abstract

The invention belongs to the field of genetic engineering and tumor medicine, and discloses a cancer-testis genetic marker related to primary lung cancer auxiliary diagnosis and application thereof. The marker is combination of rs12166583, rs12477419, rs75764106, rs74640907, rs74377505, rs1007089, rs1433646, rs6001675, rs11125117, rs3747093 and rs793598. The marker can be used for preparing primary lung cancer auxiliary diagnosis kit, and has a relatively good clinical application prospect.

Description

field of invention [0001] The invention belongs to the field of genetic engineering and tumor medicine, and relates to a cancer-testis gene SNP marker related to the auxiliary diagnosis of primary lung cancer and its application. Background technique [0002] Malignant tumor is one of the major diseases that endanger human health. Since 2010, malignant tumors have surpassed cardiovascular and cerebrovascular diseases to become the number one cause of death in my country and the most important public health problem in China. Lung cancer is one of the malignant tumors that affects the most people in the world. According to the International Agency for Research on Cancer (IARC), there were 1.825 million new lung cancer cases and 1.59 million deaths in 2012, accounting for 13.0% and 19.4% of all new cancer cases and deaths, respectively. Due to the continuous growth of tobacco consumption, environmental pollution and changes in lifestyle, the incidence of lung cancer in my cou...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
Inventor 胡志斌郭雪江霍然戴俊程王铖秦娜江玥
Owner NANJING MEDICAL UNIV
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