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A SNP marker related to auxiliary diagnosis of breast cancer and its application

An auxiliary diagnosis and marker technology, applied in the fields of genetic engineering and oncology

Active Publication Date: 2016-01-20
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no report on the application of SNP in the diagnosis of breast cancer. If the SNP of breast cancer susceptibility can be screened out as a biomarker, and the corresponding diagnostic kit can be developed, it will greatly promote the current situation of early diagnosis of breast cancer in my country. , and open up new ways for its drug screening, efficacy evaluation and targeted therapy

Method used

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  • A SNP marker related to auxiliary diagnosis of breast cancer and its application
  • A SNP marker related to auxiliary diagnosis of breast cancer and its application
  • A SNP marker related to auxiliary diagnosis of breast cancer and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0067] The collection of embodiment 1 sample and the arrangement of sample data

[0068] From January 2004 to April 2010, the inventor collected a large number of blood samples from patients with new-onset breast cancer in a hospital in Jiangsu Province. After sorting out the sample data, the inventor selected 2137 samples that met the following criteria. Experimental samples for microarray scanning and single SNPSequenomMassARRAY genotyping:

[0069] 1. Breast cancer cases diagnosed by pathology;

[0070] 2. Have not received radiotherapy or chemotherapy before blood collection, and have no previous history of cancer;

[0071] 3. Healthy female controls matched with the age of the case

[0072] The demographic data and clinical data of these samples were collected systematically.

Embodiment 2

[0073] Example 2 Whole Exome Detection of SNP in Peripheral Blood DNA

[0074] Among the 1064 eligible breast cancer patients and 1073 healthy female controls mentioned above, the two groups were balanced and comparable in age. The two groups of people were detected by the whole exome microarray to obtain relevant results. The specific steps are:

[0075] 1. Add hemolysis reagent (i.e., lysate, 40 parts) to the peripheral blood stored in a 2ml cryopreservation tube. The volume of the solution was adjusted to 2000ml, the same below), and it was completely transferred after inverting and mixing.

[0076] 2. Removal of red blood cells: Fill the 5ml centrifuge tube to 4ml with hemolysis reagent, mix by inverting, centrifuge at 4000rpm for 10 minutes, and discard the supernatant. Add 4ml of hemolysis reagent to the precipitate, invert and wash again, centrifuge at 4000rpm for 10 minutes, and discard the supernatant.

[0077]3. Extract DNA: Add 1ml of extract solution to the pre...

Embodiment 3

[0087] Example 3 SequenomMassARRAY genotyping of a single SNP

[0088] The SNPs found to be significantly associated with breast cancer in the above whole exome scan were detected on the SequenomMassARRAY genotyping platform. The specific steps were as follows:

[0089] 1. Add the hemolysis reagent to the peripheral blood stored in the 2ml cryopreservation tube, mix it upside down and transfer it completely.

[0090] 2. Removal of red blood cells: Fill the 5ml centrifuge tube to 4ml with hemolysis reagent, mix by inverting, centrifuge at 4000rpm for 10 minutes, and discard the supernatant. Add 4ml of hemolysis reagent to the precipitate, invert and wash again, centrifuge at 4000rpm for 10 minutes, and discard the supernatant.

[0091] 3. Extract DNA: Add 1ml of extract solution and 8μl of proteinase K to the precipitate, shake and mix well on the shaker, and bathe in 37°C water overnight.

[0092] 4. Remove protein: add 1ml of saturated phenol and mix well (shake gently by h...

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Abstract

The invention belongs to the fields of gene engineering and oncology, and discloses a mammary cancer assisted diagnosis related SNP (single-nucleotide polymorphism) marker and application thereof. The marker is a combination of 32 SNPs, such as rs10152591, rs1037699, rs10487075, rs11645106, rs11663688, rs11768670, rs11910707, rs12972670, rs13038095, rs142255517, rs142895740, rs144600021, rs17128116, rs2053026, rs213212, rs2229300, rs2981582, rs35002951, rs3748140 and the like. The marker can be used for preparing a mammary cancer assisted diagnosis kit.

Description

technical field [0001] The invention belongs to the fields of genetic engineering and oncology, and relates to a SNP marker related to auxiliary diagnosis of breast cancer and its application. Background technique [0002] Breast cancer is the most common malignant tumor in women, which seriously threatens women's health. According to statistics, there were 1.68 million new cases of breast cancer in the world in 2012, accounting for about 25.2% of all new cases of malignant tumors in women. Although the incidence of breast cancer in my country is lower than that of western developed countries, it has been increasing at an annual rate of 3-5% in the past two decades, much higher than the world average growth rate of 0.5%. Although breast cancer has a better prognosis, it is still the leading cause of death from malignant tumors in women due to its high incidence. In 2012, 48,000 women died of breast cancer in my country. Therefore, breast cancer has become the primary mali...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 沈洪兵胡志斌马红霞靳光付陈佳萍何伊莎
Owner NANJING MEDICAL UNIV
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