Serum exosome tsRNA marker related to breast cancer diagnosis and application thereof

A marker and exosome technology, applied in the field of molecular biology and oncology, can solve the problems of poor sensitivity and specificity, high equipment requirements, complicated operation, etc., and achieve the effect of high sensitivity and specificity

Pending Publication Date: 2021-09-21
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, imaging examination, detection of breast cancer markers and needle biopsy are commonly used clinical methods for breast cancer diagnosis, but these methods have certain limitations: Imaging methods such as PET-CT can diagnose and detect small tumors, but require high equipment And the operation is cumbersome, unable to reflect the actual therapeutic effect at the molecular level, and its popularization and application are limited; some conventional breast cancer serum markers such as glycoprotein CA153, carcinoembryonic antigen CEA, etc. can be used for breast cancer diagnosis, but their sensitivity and poor specificity
Recent studies have found that tsRNAs can also exist stably and are abundant in serum. However, the research on tsRNA in exosomes and breast cancer has not been reported so far. Markers, and the development of corresponding diagnostic kits, are necessary for the diagnosis and treatment of breast cancer

Method used

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  • Serum exosome tsRNA marker related to breast cancer diagnosis and application thereof
  • Serum exosome tsRNA marker related to breast cancer diagnosis and application thereof
  • Serum exosome tsRNA marker related to breast cancer diagnosis and application thereof

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Embodiment

[0040] A serum exosomal tsRNA marker for the diagnosis of breast cancer, the marker is a combination of the following three sequences:

[0041] tRF-Glu-TTC-009: SEQ ID No.1;

[0042] tRF-Gly-TCC-010: SEQ ID No.2;

[0043] tRF-His-GTG-005: SEQ ID No.3.

[0044] The present invention provides the stem-loop primers of the above-mentioned serum exosome tsRNA markers, the stem-loop primers are:

[0045] The stem-loop primer of tRF-Glu-TTC-009 is SEQ ID No.4;

[0046] The stem-loop primer of tRF-Gly-TCC-010 is SEQ ID No.5;

[0047] The stem-loop primer of tRF-His-GTG-005 is SEQ ID No.6.

[0048] The present invention provides primers for the above-mentioned serum exosome tsRNA markers, the primers are:

[0049] The forward primer of tRF-Glu-TTC-009 is SEQ ID No.7;

[0050] The forward primer of tRF-Gly-TCC-010 is SEQ ID No.8;

[0051] The forward primer of tRF-His-GTG-005 is SEQ ID No.9;

[0052] The reverse primer is a universal primer sequence, and its sequence is shown in...

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Abstract

The invention discloses a serum exosome tsRNA marker related to breast cancer diagnosis and application thereof, and belongs to the field of molecular biology and oncology. The marker is a combination of tRF-Glu-TTC-009, tRF-Gly-TCC-010 and tRF-His-GTG-005 in a serum exosome tsRNA. The marker has relatively high sensitivity and specificity and is beneficial to auxiliary diagnosis of breast cancer.

Description

technical field [0001] The invention belongs to the fields of molecular biology and oncology, and relates to a serum exosome tsRNA marker related to breast cancer and an application thereof. Background technique [0002] Breast cancer is the type of tumor with the highest morbidity and mortality among women. According to statistics, the annual incidence of breast cancer in the world is 2.1 million and the death toll is 630,000, ranking first among female malignant tumors. At present, although breast cancer has a better prognosis, it is still the leading cause of cancer death in women due to its high incidence. [0003] At present, imaging examination, detection of breast cancer markers and needle biopsy are commonly used clinical methods for breast cancer diagnosis, but these methods have certain limitations: Imaging methods such as PET-CT can diagnose and detect small tumors, but require high equipment And the operation is cumbersome, unable to reflect the actual therapeut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6851C12N15/11
CPCC12Q1/6886C12Q1/6851C12Q2600/158C12Q2600/178C12Q2531/113C12Q2521/107C12Q2525/301
Inventor 金芳芳陈曦
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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