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SNP marker associated with auxiliary diagnosis of esophageal cancer

A technology for auxiliary diagnosis, esophageal cancer, applied in the field of tumor medicine

Inactive Publication Date: 2019-09-10
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no effective preparations for the early diagnosis and general screening of esophageal cancer. If the susceptibility sites of esophageal cancer can be screened out as biomarkers, and the corresponding diagnostic kits can be developed, it will be a powerful contribution to the current situation of early diagnosis of esophageal cancer in my country. It also opens up new ways for its drug screening and precision treatment

Method used

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  • SNP marker associated with auxiliary diagnosis of esophageal cancer
  • SNP marker associated with auxiliary diagnosis of esophageal cancer
  • SNP marker associated with auxiliary diagnosis of esophageal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1: collection of samples and arrangement of sample data

[0074] The inventor collected a large number of blood samples from patients with esophageal cancer from the First Affiliated Hospital of Zhengzhou University from 2016 to 2018. After sorting out the sample data, the inventor selected 6156 samples (2575 cases of esophageal cancer and 3581 cases of Healthy controls) were used as experimental samples for genome-wide microarray scanning and single SNP Sequenom MassARRAY genotyping, and the demographic and clinical data of these samples were systematically collected. Among them, the selection criteria of 6156 samples were as follows:

[0075] (1) Esophageal cancer cases diagnosed by pathology, who have not received radiotherapy or chemotherapy before blood collection, and have no previous history of cancer;

[0076] (2) Healthy controls matched with age and sex of esophageal cancer cases.

Embodiment 2

[0077] Example 2: Genome-wide scanning of SNPs in peripheral blood DNA

[0078] From 6156 experimental samples, 1026 patients with esophageal cancer and 1573 healthy controls were selected, and the age and gender balance of the two groups were comparable. The two groups of people were detected by Illumina660 chip to obtain relevant results. The specific steps are:

[0079] 1. Extract DNA: Extract sample DNA according to the instructions of the QIAGEN Genomic DNA Extraction Kit. The specific steps are as follows:

[0080] (1) Cell thawing: Take the blood sample out of the -80°C refrigerator, and place it in a -20°C low-temperature refrigerator for 24 hours, and then in a 4°C refrigerator for 24 hours;

[0081] (2) Removal of red blood cells: Take 1.0ml FG1buffer and 400μl blood samples in turn and add them to a new EP tube, invert up and down evenly 20-30 times, mix until no blood clots and particles appear with the naked eye; centrifuge at 4°C for 7 minutes at 15,000rpm, Di...

Embodiment 3

[0092] Example 3: Sequenom MassARRAY genotyping of individual SNPs

[0093] The SNPs found to be related to the onset of esophageal cancer through whole-genome scanning in Example 2 were detected in another 1549 cases of esophageal cancer and 2008 healthy controls, and the specific steps were as follows:

[0094] 1. DNA extraction: Extract sample DNA according to the instructions of the QIAGEN Genomic DNA Extraction Kit. The specific operation is the same as the DNA extraction step in Example 1, and will not be repeated here.

[0095] 2. Measurement concentration: Usually 15-25ng / μl DNA can be obtained, and the purity (ultraviolet 260OD / 280OD ratio and 260OD / 230OD ratio) is 1.8-2.0;

[0096] 3. Perform Sequenom MassARRAY genotyping:

[0097] (1) use MassARRAY Assay Design software to design polymerase chain reaction (Polymerase Chain Reaction, PCR) specific amplification primers and single-base specific extension primers for 11 positively associated SNPs found in genome-wide sc...

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Abstract

The invention belongs to the technical field of oncology medicine, and specifically discloses an SNP marker associated with the auxiliary diagnosis of esophageal cancer. The SNP marker is a combination of rs10052657, rs10058728, rs2014300, rs4072037, rs2239815, rs9288318, rs3763338, rs9868873, rs3781264, rs4822983 and rs8030672. The SNP marker combination can be used for assisting in clinical screening diagnosis of the esophageal cancer, helps to reflect the disease state of asymptomatic high-risk groups suffering from the esophageal cancer, and provides support for clinicians to quickly and accurately know the disease conditions of patients and timely take more personalized prevention and treatment schemes.

Description

technical field [0001] The invention belongs to the field of tumor medicine, and in particular relates to a SNP marker related to the auxiliary diagnosis of esophageal cancer. Background technique [0002] Esophageal cancer is one of the most common digestive system malignancies worldwide. According to the latest data from the World Health Organization (WHO) in 2018, there are 570,000 new cases of esophageal cancer each year in the world, and about 510,000 deaths, ranking seventh in cancer incidence and sixth in mortality worldwide. According to the analysis of the incidence and death of malignant tumors in different regions in China in 2015 released by the National Cancer Center, the number of new cases of esophageal cancer in my country accounts for half of the world. [0003] Since the onset of esophageal cancer is very insidious, early detection and diagnosis are extremely difficult. Clinically, most of the first-diagnosed patients are in the middle and advanced stages,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 王立东王盼盼赵学科宋昕张亚丽杨道科李健
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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