Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Early stomach cancer assisted diagnosis related SNP (single-nucleotide polymorphism) marker and application thereof

A technology for auxiliary diagnosis and early gastric cancer, applied in the field of SNP markers

Active Publication Date: 2015-01-14
NANJING MEDICAL UNIV
View PDF1 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there is no report on the application of SNP in the diagnosis of early-onset gastric cancer. If SNPs that are susceptible to early-onset gastric cancer can be screened out as biomarkers, and corresponding diagnostic kits can be developed, it will be a powerful contribution to the diagnosis of gastric cancer in my country. The promotion of the drug has also opened up a new way for its drug screening, drug efficacy evaluation and targeted therapy

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Early stomach cancer assisted diagnosis related SNP (single-nucleotide polymorphism) marker and application thereof
  • Early stomach cancer assisted diagnosis related SNP (single-nucleotide polymorphism) marker and application thereof
  • Early stomach cancer assisted diagnosis related SNP (single-nucleotide polymorphism) marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] The collection of embodiment 1 sample and the arrangement of sample data

[0077] The inventor collected blood samples from patients with gastric cancer from the Nanjing Medical University Cancer Center from 2004 to 2010. After sorting out the sample data, the inventor selected 287 samples that met the following criteria: Whole-genome chip scan and single SNP Sequeom Experimental samples for MassARRAY genotyping:

[0078] 1. Gastric cancer patients with definite pathological diagnosis, who are younger than 50 years old;

[0079] 2. Healthy controls matched with the age and sex of the cases;

[0080] The demographic data and clinical data of these samples were collected systematically.

Embodiment 2

[0081] Whole Genome Scanning of SNP in Example 2 Peripheral Blood DNA

[0082] Among the 129 gastric cancer patients and 158 healthy controls who met the above conditions, the two groups were matched in age and gender. The two groups of people were detected by Affymetrix6.0 chip to obtain relevant results. The specific steps are:

[0083] 1. Add hemolysis reagent (i.e., lysate, 40 parts) to the peripheral blood stored in a 2ml cryopreservation tube. The volume of the solution was adjusted to 2000ml, the same below), and it was completely transferred after inverting and mixing.

[0084] 2. Removal of red blood cells: Fill the 5ml centrifuge tube to 4ml with hemolysis reagent, mix by inverting, centrifuge at 4000rpm for 10 minutes, and discard the supernatant. Add 4ml of hemolysis reagent to the precipitate, invert and wash again, centrifuge at 4000rpm for 10 minutes, and discard the supernatant.

[0085] 3. Extract DNA: Add 1ml of extract solution to the precipitate (each 3...

Embodiment 3

[0094] Example 3 Sequeom MassARRAY genotyping of a single SNP

[0095] The SNPs found to be associated with the onset of gastric cancer in the above genome-wide scan were detected in 129 gastric cancer cases and 158 healthy controls. The specific steps were as follows:

[0096] 1. Add the hemolysis reagent to the peripheral blood stored in the 2ml cryopreservation tube, mix it upside down and transfer it completely.

[0097] 2. Removal of red blood cells: Fill the 5ml centrifuge tube to 4ml with hemolysis reagent, mix by inverting, centrifuge at 4000rpm for 10 minutes, and discard the supernatant. Add 4ml of hemolysis reagent to the precipitate, invert and wash again, centrifuge at 4000rpm for 10 minutes, and discard the supernatant.

[0098] 3. Extract DNA: Add 1ml of extract solution and 8μl of proteinase K to the precipitate, fully oscillate and mix on a shaker, and bathe overnight at 37°C.

[0099] 4. Remove protein: add 1ml of saturated phenol and mix well (shake gently...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the fields of gene engineering and tumor medicine, and discloses an early stomach cancer assisted diagnosis related SNP (single-nucleotide polymorphism) marker and application thereof. The marker is a combination of rs10737808, rs10193016, rs7607715, rs2904350, rs17312167, rs13168609, rs561443, rs7810066, rs10257532, rs3849222, rs11817406, rs7193854, rs12447431 and rs4790224. The marker can be used for preparing an early stomach cancer assisted diagnosis kit.

Description

field of invention [0001] The invention belongs to the fields of genetic engineering and tumor medicine, and relates to a SNP marker related to the auxiliary diagnosis of early gastric cancer and its application. Background technique [0002] Gastric cancer is one of the most common malignant tumors worldwide. According to the World Health Organization, there were about 630,000 new cases of gastric cancer worldwide in 2012, accounting for 8.5% of all tumors, second only to lung cancer, prostate cancer and colorectal cancer. Nearly 50% of gastric cancer cases worldwide occur in China. In my country, the age-standardized incidence rate of gastric cancer is 22.7 / 100,000, second only to lung cancer. About 90%-95% of gastric cancers are adenocarcinomas, originating from the gastric epithelium. Anatomically, it can be divided into cardia cancer and non-cardia cancer. Cardiac carcinoma is an adenocarcinoma occurring within 2 cm below the esophagogastric junction in the gastric ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 沈洪兵靳光付马红霞胡志斌戴俊程杜江波
Owner NANJING MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com