33results about How to "Help with assessment" patented technology

Provided is a method of attaching advertising red envelopes in social networking service, comprising: manufacturing an advertising red envelope including advertising contents and red envelopes, issuing the advertising red envelope in a social network system, identifying users obtaining the advertising red envelope and watching related advertisements on a social network, and awarding the users obtaining the advertising red envelope and watching the advertising contents. The system comprises a storage medium; and at least one processor for reading the storage medium, and performing the following operation: making an advertising red envelope including advertising contents and red envelopes, issuing the advertising red envelope in a social network system, identifying users obtaining the advertising red envelope and watching related advertisements on a social network, and awarding the users obtaining the advertising red envelope and watching the advertising contents. The method can be used as an advertising system with spot award, increase the rate of converting advertisement watching users into goods and service buyers, reduce unnecessary advertising expense, and improving advertisement effectiveness on a social network.

The invention discloses an LncRNA combination for detecting a prognosis condition of stomach cancer and a kit containing the combination. According to the invention, a fluorescent quantitation PCR or digital PCR technique is adopted to identify the difference change of a set of specific LncRNA expression quantity in a stomach cancer patient sample (tissue, plasma, serum, gastric juice, and the like) and a corresponding paracancerous sample or normal sample and to early and accurately evaluate the risk of stomach cancer relapse or transfer. The kit disclosed by the invention provides a biomarker LncRNA combination for detecting the prognosis condition of stomach cancer, a primer for detecting the LncRNA contained in the combination and a related reagent, so that the kit is capable of effectively increasing the detection efficiency and accuracy for the stomach cancer prognosis relapse or transfer. The invention adopts a set of prognosis transfer related LncRNA combination for avoiding the defect of great increasing of the fault diagnosis rate and missed diagnosis rate of the stomach cancer diagnosis caused by lower sensitivity and specificity resulted from a detection index LncRNA served as a tumor marker kit.

The invention discloses a TKY pipe joint welding seam phased array ultrasonic beam covering method, the steps are as follows: according to the physical size of the pipe joint welding seam, the section figure at the specific position of the pipe joint is obtained; the weld joint outline is drawn in the obtained section picture; Obtain the phased array probe length, front length, height, inclination angle, and array element center height; select the incident point of the main sound beam of the array element on the ellipse of the outer wall of the branch pipe of the obtained graphic, and make the tangent line of the ellipse of the outer wall of the branch pipe at this point; the determined incidence The point is the elliptical contact point between the outline of the phased array probe and the outer wall of the branch pipe, and the tangent direction is the length direction of the phased array probe. Draw the outline of the phased array probe and draw the position of the array element; select the sound beam drawing method to draw the sound beam line. The invention contributes to the evaluation of the blind area of ​​TKY pipe joint weld ultrasonic detection, determines the coverage state of the ultrasonic beam on the pipe joint welding joint, effectively reduces the probability of missed detection or misjudgment of pipe joint detection, and contributes to the phase control of the TKY pipe joint weld Process design and defect assessment for array ultrasonic testing.

The invention provides a method and a system for preventing vehicle mileage tampering. The method comprises the following steps of: obtaining mileage record data of a vehicle; performing data checking calculation on the obtained mileage record data and then obtaining a checking value; performing coding processing on the mileage record data and the checking value obtained after the calculation to form mileage record data backup and storing the mileage record data backup, wherein the storage position of the mileage record data backup is different from a conventional storage position of the mileage record data; during vehicle ignition starting, comparing the mileage corresponding to the mileage record data backup with the mileage obtained from the conventional storage position, and comparing the checking value obtained after the calculation with the checking value corresponding to the mileage obtained from the conventional storage position; if the mileages are consistent and the checking values are consistent, normally displaying the mileage obtained from the conventional storage position by a vehicle instrument; and if at least one item is inconsistent, displaying error prompt through the vehicle instrument. Therefore whether the vehicle running mileage is artificially modified or not can be known, so that the vehicle running mileage is prevented from being illegally tempered.

The invention relates to a chronic kidney disease renal function diagnostic kit based on lncRNA-H19 and application of the lncRNA-H19. The kit comprises an RAN extracting agent, an lncRNA-H19 primer, a reverse transcription system and a standard PCR (Polymerase Chain Reaction) system. An experiment finds out that the expression condition of the lncRNA-H19 in blood serum of a patient has positive correlation with the development of a chronic kidney disease renal function; when the expression of the lncRNA-H19 is higher, the renal function of the patient is better; after treatment, the prognosis of the patient is better when the effect is better; the lncRNA-H19 is promoted to be used as a checking standard for helping the diagnosis on the chronic kidney disease renal function, so that the lncRNA-H19 can be used as a related index for diagnosing the chronic kidney disease renal function and can be used for manufacturing a chronic kidney disease renal function diagnostic reagent and the diagnostic kit.

The invention provides an intelligent visual electromagnetic radiation detection device comprising a receiving module and an analysis module. The receiving module includes an antenna. The analysis module consists of an amplifier module, a signal processing module, a main control module and a display module. The antenna, the amplifier module, the signal processing module, the main control module, and the display module are connected successively. The main control module generates a first electromagnetic radiation waveform according to measured electromagnetic radiation data and sends the first electromagnetic radiation waveform to the display module. According to the invention, an electromagnetic radiation waveform in a measuring area can be displayed in real time, so that a user can sense changing of a surrounding electromagnetic radiation environment visually. Moreover, the operating method is simple; measurement of a specific area can be completed without too much manual intervention, so that the user experience is improved substantially.

The present invention discloses a GFAP detection method based on a fluorescence resonance energy transfer method, wherein a micro-pore plate is coated with a purified glial fibrillary acidic protein (GFAP) antibody to prepare a solid-phase antibody, glial fibrillary acidic protein (GFAP) is sequentially added to the monoclonal antibody-coated micro-pores, combination with Alexa Fluor488 labeled and Alexa Fluor594 labeled GFAP antibody to form an antibody-antigen two-fluorescent-labeling-antibody complex, absorbance (OD value) is determined by using a fluorescent enzyme analyzer according to the FRET principle, and the human glial fibrillary acidic protein (GFAP) content in the sample is calculated by using a standard curve. According to the present invention, with the use of the three kinds of the antibodies to simultaneously identify, such that the specificity is high, the accuracy is good, the detection time is substantially shortened compared to the existing method, the reference information is provided for the clinical diagnosis, the clinicians can be helped to diagnose and assess the progression of stroke, and the prognosis of patients can be improved.

The related E-business system comprises: at least one superior provider, sole distributor or general agent as the superior providing end connected with a shopping platform; a goods database on the platform to store the provider information, reference price and preferential data; a query communication device to connect the consumer and platform and accept the input, such as position and name, and obtain the at least one local shopping data or map. This invention increases consumption security and accuracy.

The invention relates to the fields of biotechnology and medical diagnosis, and concretely relates to intrahepatic duct cell cancer related gene mutation targets and an application of the gene mutation targets in reagents or kits for diagnosing or treating intrahepatic duct cell cancer and assessing intrahepatic duct cell cancer prognosis. The intrahepatic duct cell cancer related gene mutation targets TP53, KRAS, IDH1, PTEN, ARID1A, EPPK1, ECE2 and FYN are found in the invention, and the gene mutation targets can be effectively used in development judgment, treatment scheme selection and / or prognosis assessment of the intrahepatic duct cell cancer, so a new intrahepatic duct cell cancer diagnosis agent and / or treatment agent are / is provided for the fields, and have / has certain clinic application prospect.

The invention discloses a method for evaluating biological integrity of benthonic animals of a freshwater ecosystem based on environmental DNA macro bar code monitoring data, and belongs to the technical field of biology. Based on the results of monitoring of the benthonic animals through environmental DNA macro bar codes, the method comprises a series of monitoring and evaluation processes including (1) determining a reference point and a damaged point; (2) collecting an environmental DNA sample; (3) performing biological monitoring on an eDNA macro bar code; (4) defining and calculating candidate biological indexes based on the eDNA macro bar code monitoring; (5) screening the candidate indexes; (6) selecting the biological indexes to calculate the biological integrity score of the benthonic animals; and (7) dividing health levels and the like. The method provides complete and easy-to-operate guidance for the evaluation of the biological integrity of the benthonic animals of the freshwater, and can be widely applied to the evaluation of the integrity of the benthonic animals and the evaluation of the water ecological health condition of freshwater ecosystems such as streams, rivers, lakes and reservoirs.

The invention provides a multi-loop and unequal-section cable cluster laying method. The method comprises the steps that the cable cluster laying mode, environment parameters and structural parameters and calculation parameters of cables in a cable cluster are obtained; a cable cluster carrying capacity optimized design mathematic model is established according to the cable cluster laying mode, the environment parameters and the structural parameters and the calculation parameters of the cables in the cable cluster; iteration is carried out through a barrier function, the cable cluster carrying capacity optimized design mathematic model is solved, and the cable cluster carrying capacity is obtained; whether laying of the multi-loop and unequal-section cable cluster is reasonable or not is judged according to the carrying capacity of the cable cluster, and if not, the cable cluster carrying capacity and / or arrangement are / is adjusted. Optimization from two aspects including the carrying capacity running optimization and line arrangement optimization can be achieved through the multi-loop and unequal-section cable cluster laying method. The dynamitic running load is more flexible and accurate. Meanwhile, the optimization result can serve as a cable dispatching running reference basis.

The invention discloses a method for determining the biological availability of a mouse with nickel in soil and food. The method comprises the following specific steps: preparing nickel-containing food for feeding mice; collecting mouse urine, finally, acquiring the relative biological effectiveness of the mice with nickel in soil and food. The method is improved and innovated on the basis of a conventional experimental method. A method capable of accurately and effectively measuring the biological effectiveness of the mouse with nickel in soil and food is designed, namely, the end point of nickel accumulation is positioned in urine, the amount of nickel absorbed by the mouse is obtained by collecting the urine of the mice and measuring the content of nickel in the urine, and therefore therelative biological effectiveness of the mice with nickel is calculated.

The invention relates to an offshore wind power digital twin test pile test system and an establishment method, the offshore wind power digital twin test pile test system comprises a loading mechanism, a data acquisition mechanism, a calculation and analysis platform and a digital twin model, the data acquisition mechanism is connected with the loading mechanism, and the calculation and analysis platform is connected with a jack of the loading mechanism and the data acquisition mechanism to form an on-site test pile test system, the digital twinborn model is a numerical simulation model constructed based on an on-site pile test system, model parameters are updated in real time by adopting on-site pile test data, and the digital twinborn model is established and operated on a computational analysis platform. High-precision design of an offshore wind power pile foundation structure is facilitated, a method is provided for understanding a stress deformation mechanism of a large-diameter or super-large-diameter pile body under the complex load working condition, meanwhile, stress visualization of soil around the pile and real-time analysis of stress deformation of the pile body are achieved based on numerical simulation of a digital twinborn model, and an existing offshore wind power pile foundation design method can be evaluated, checked and corrected.

The invention provides a kit for highly-sensitively detecting the number and the functions of T cells. The kit comprises a micro-porous filter membrane double-chamber culture dish, a culture medium for a double-chamber filter membrane system, a T cell culture solution, a 96-well plate coated with an ELISPOT culture plate of anti-human IFN-gamma, TNF-alpha, IL-2, CD4 and CD8 monoclonal antibodies and a cellulose membrane, a mouse anti-human CD3 monoclonal antibody solution, AP enzyme-labeled streptavidin, and a chromogenic solution. The invention also provides a method for detecting the T cells. The kit and the method are capable of highly-efficiently detecting the T cells.

The invention provides a cell circular scar healing experimental device for a six-pore plate. The device comprises a flake (1), wherein a criss-cross structure (2) is arranged at the bottom of the flake; a cylindrical structure (3) is arranged at the bottom of a cross end of the criss-cross structure. The device is convenient for observing scar healing in the whole visual field, and avoids the phenomenon that only local area healing situation of a scar can be observed in existing cell scar experiments.

The invention belongs to the technical field of gas pipeline explosion venting, and particularly relates to a numerical simulation method in a gas pipeline explosion venting process, which comprises the following steps: establishing a model, namely establishing a basic model on the basis that the gas explosion venting process is a rapid combustion reaction process and meets mass conservation, momentum conservation, energy conservation and chemical component equilibrium equations; setting a calculation area and conditions, carrying out numerical simulation on a gas explosion experiment, and respectively carrying out explosion venting of a single explosion venting port and explosion venting of two explosion venting ports; analyzing a simulation result, and comparing a numerical simulation result with gas explosion test data; obtaining the conclusion that the position of the explosion venting opening has important influence on the detonation-to-detonation conversion process of the hydrogen-air mixture in the pipeline. According to the invention, a mode of combining multiple models is adopted in the numerical simulation process, numerical simulation is carried out on the conditions of a single explosion venting opening and two explosion venting openings, the influence of different numbers of explosion venting openings on the explosion venting process is determined, and a corresponding conclusion is obtained.

The embodiment of the invention discloses a simulation recommendation method, computer equipment and a storage medium. One specific embodiment of the simulation recommendation method comprises the steps of displaying user information, a user information modification control and recommendation object information; obtaining modified user information in response to an operation on the user information modification control or in response to operation data received from the user terminal; and calculating to obtain recommendation object information corresponding to the modified user information, andsynchronously displaying the modified user information and the recommendation object information corresponding to the modified user information. According to the simulation recommendation method, thechange of the recommendation object result caused by the modification of the user information is simulated and presented in a visual mode, the system logic of the simulation recommendation method isevaluated, the algorithm vulnerability is discovered and corrected in time, and meanwhile, different recommendation strategies are compared, so that the recommendation accuracy is improved.Commodity labels can be better managed, and personalized operation strategies can be better formulated.

The invention discloses an electronic product comprehensive stress dust test evaluation method, and belongs to the field of reliability test. The method comprises the following steps: firstly, carrying out investigation and information collection on an actual application environment of a to-be-evaluated electronic product, and carrying out classified statistical analysis on related data; secondly, according to a data analysis result in the first step, comprehensive stress sand and dust test severity selection and test profile design are carried out; then, according to the test conditions and the profile designed in the step 2, specific implementation of the test and monitoring of key parameters are carried out; and finally, carrying out performance characterization test and risk grade judgment on the test sample. Based on the actual application environment characteristics of the product, the temperature stress and sand dust comprehensive stress condition is introduced, and the risk grade of the product is evaluated under the temperature and humidity conditions, so that the application environment of the product can be simulated more appropriately, and the reliability of the electronic product under the comprehensive sand dust stress condition is evaluated more perfectly.

Disclosed is an in-service air cooler tube bundle detection method based on an X-ray digital imaging technology; the detection method comprises the following steps that a detection object is determined, and detection equipment and tools are prepared; an X-ray machine and a flat-plate detector are fixed on the corresponding tools, and the tools are arranged on the detected air cooler; and the corresponding detection parameters of the X-ray machine are selected and the tools are adjusted to ensure that the requirement of the detection focal length is met; the sensitivity of the detection systemis determined by using an image quality meter; the effective detection area of each time of detection, the number of the tube bundles of the air cooler, and the simultaneous stepping lengths of the X-ray machine and the flat panel detector are determined, and then detection is carried out; whether the defect exists in the tube bundle of the detected air cooler or not is judged according to a digital imaging result on a displayer; after the suspected defect is positioned, defect quantification is carried out, and whether the tube bundle of the air cooler has an exceed standard defect or not isjudged according to the positioning and quantification of the defect. According to the method, the corrosion condition of the tube bundle of the air cooler can be quickly and effectively known, and the influence of the overlap of the outer fins and the multiple rows of tube bundles on the detection can be effectively avoided.

The fireproof simulation analysis method of the fuel and lubricating oil pipeline comprises the following steps: acquiring a flame burner and a standard flame based on the flame burner in a simulation environment, placing the fuel and lubricating oil pipeline at a set position above the flame burner for flame thermal shock simulation, and extracting temperature data of the fuel and lubricating oil pipeline; dividing a flame impact area of the pipeline into a control body, solving an energy conservation equation of the control body to obtain a working medium outlet temperature of the fuel-lubricating oil pipeline, and preliminarily judging whether the working medium in the fuel-lubricating oil pipeline has phase change or not by taking a boiling point as a judgment criterion; the method comprises the following steps: firstly, calculating a steady-state flow field in a fuel lubricating oil pipeline without adding flame to obtain a steady-state flow field calculation result; and on the basis of the calculation result of the steady-state flow field, carrying out thermal-state heat transfer solution. According to the method, the physical process of the fire prevention simulation of the fuel and lubricating oil pipeline can be decoupled, so that the fire prevention test of the fuel and lubricating oil pipeline is accurately simulated to support the fire prevention airworthiness evidence obtaining work of the fuel and lubricating oil pipeline.

The invention discloses a detection method for colorectal cancer polygene ctDNA, which specifically comprises the following steps: S1, collecting a pre-treatment tissue specimen; S2, treating a blood sample; S3, performing cfDNA extraction; S4, measuring the concentration and detecting the size of a fragment; S5, performing mixing and centrifuging; S6, performing a ddPCR detection stage; S7, reading a fluorescence signal; and S8, analyzing a numerical result. The invention relates to the technical field of molecular diagnosis gene detection. According to the detection method for the colorectal cancer polygene ctDNA, ddPCR amplification is performed on cfDNA through a specific detection primer and a probe of a reference gene beta-Actin; an amplification product is detected; finally, fluorescence signal data is analyzed; fluorescence information of KRAS, NRAS, PIK3CA and BRAF genes and the reference gene beta-Actin is obtained; plasma ctDNA possibly replaces tumour tissue biopsy; and as a molecular marker for auxiliary diagnosis of colorectal cancer, change of RAS (KRAS, NRAS), BRAF and PIK3CA gene mutation states in ctDNA of a patient before and during cetuximab treatment is dynamically monitored.

The invention relates to an electric heating parameter obtaining method of a superconducting cable. The method comprises the following steps: S1, obtaining structural parameters, material characteristics and line laying conditions of the superconducting cable; s2, obtaining boundary conditions according to laying conditions, and obtaining a constitutive equation according to structure parameters and material characteristics; s3, establishing a two-dimensional finite element model according to the constitutive equation, the boundary condition and the partial differential equation equivalent resistivity; s4, performing transient solution of the two-dimensional finite element model according to the current of the superconducting cable to obtain an alternating current loss characteristic curve of the superconducting cable; and S5, performing steady-state solution of the two-dimensional finite element model according to the alternating-current loss characteristics of the superconducting cable to obtain temperature field distribution of the superconducting cable in the steady-state operation process. Compared with the prior art, the complexity of calculation is reduced, and the convergence of the model is improved.

The invention belongs to the field of biological medicine, and mainly relates to application of a vascular secretion factor in preparation of a biomarker for detecting non-alcoholic steatohepatitis. The invention provides an application of a vascular secretion factor in preparation of a biomarker for detecting non-alcoholic steatohepatitis, and is characterized in that the vascular secretion factor comprises IGFBP7 and / or ADAMTS1; the IGFBP7 and / or the ADAMTS1 are / is expressed in an HDAC2 (histone deacetylase 2) / DNMT1-IGFBP7 / ADAMTS1-Th17 signal channel. The invention also provides application of the vascular secretion factor in preparation of the biomarker for distinguishing the non-alcoholic steatohepatitis and the simple fatty liver, and is characterized in that the vascular secretion factor comprises IGFBP7 and / or ADAMTS1; the IGFBP7 and / or the ADAMTS1 are / is expressed in an HDAC2 (histone deacetylase 2) / DNMT1-IGFBP7 / ADAMTS1-Th17 signal channel. The invention further provides application of the synergistic effect of the IGFBP7 and / or the ADAMTS1 in preparation of the biomarker for detecting the non-alcoholic steatohepatitis. According to the application provided by the invention, the non-alcoholic steatohepatitis can be evaluated more reliably, simply, conveniently and noninvasively.

The invention provides a kit for highly-sensitively detecting the number and the functions of T cells. The kit comprises a micro-porous filter membrane double-chamber culture dish, a culture medium for a double-chamber filter membrane system, a T cell culture solution, a 96-well plate coated with an ELISPOT culture plate of anti-human IFN-gamma, TNF-alpha, IL-2, CD4 and CD8 monoclonal antibodies and a cellulose membrane, a mouse anti-human CD3 monoclonal antibody solution, AP enzyme-labeled streptavidin, and a chromogenic solution. The invention also provides a method for detecting the T cells. The kit and the method are capable of highly-efficiently detecting the T cells.

The invention relates to a kit for detecting the STR (Short Tandem Repeat) genetype of human chromosomes 21, 18 and 13 and sex chromosomes, and particularly relates to a QF-PCR (Quantitative Fluorescence-Polymerase Chain Reaction) kit for detecting the number of the chromosomes 21, 18 and 13 and the sex chromosomes by adopting five-color fluorescence labeling monotube fast multiple amplification and mainly for diagnosing 21 trisomy syndrome, 18 trisomy syndrome, 13 trisomy syndrome and the aneuploid abnormality of the sex chromosomes. The kit comprises a primer mixture, a hot start C-Taq enzyme, an amplification reaction solution, a positive quality control product, a negative reference product, a fluorescence interior label Siz-500 and an allelic gene typing standard substance. Compared with the traditional antenatal diagnosis method, the kit disclosed by the invention can realize high-flux, fast, reliable and standardized detection.

The invention relates to the technical field of medical biological detection, in particular to gene mutation targets related to paroxysmal motor-induced dyskinesias, and the use of these gene mutation targets in diagnosing or treating paroxysmal motor-induced dyskinesias and evaluating paroxysmal motor-induced dyskinesias. Use of a reagent or kit for prognosis of movement disorders. The present invention has discovered the mutation targets c.1012+5g>a, c.959C>T, c.971_972insG and c.761C>T of the main pathogenic gene PRRT2 gene of paroxysmal movement-induced dyskinesia. The mutation targets of these genes The points can be effectively used in the development judgment, treatment plan selection and / or prognosis evaluation of paroxysmal movement-induced dyskinesia, and have a certain clinical application prospect.

The invention discloses a method for measuring the biological availability of nickel in soil and food in mice. The specific steps are to make food containing nickel to feed the mice, and collect the urine of the mice to finally obtain the mice containing nickel in soil and food. Relative to the bioavailability, the present invention improves and innovates on conventional experimental methods, and designs a method that can accurately and effectively measure the bioavailability of nickel in soil and food in mice, that is, the end point of nickel accumulation is located in the urine, through The urine of the mice was collected, and the nickel content in the urine was measured to obtain the amount of nickel absorbed by the mice, so as to calculate the relative biological availability of nickel in the mice.

The invention discloses a construction method and application of a megacolon animal model. Specifically, benzalkonium chloride is injected into mice at 5-8 days old, 12-15 days old and 19-21 days old through anus perfusion. According to the method, the BALB / C suckling mouse is used for modeling the megacolon model for the first time, clysis is used as a means, surgical operation is not needed, the operation difficulty is reduced, the modeling success rate is high, the BALB / C suckling mouse of 5-8 days old is selected as an experimental subject, benzalkonium chloride anus perfusion is carried out under the condition that the intestinal tract development is not mature, intestinal neurons are damaged, and the method is closer to the HSCR attack microenvironment; the method is more suitable for clinical HSCR diagnosis and treatment age, and is beneficial to HSCR pathological mechanism research and experimental optimization and evaluation of novel treatment means. And a good research model is provided for clinical HSCR pathological mechanisms and further diagnosis and treatment. And the diversity of the megacolon animal model is enriched.