LncRNA combination for detecting prognosis condition of stomach cancer and kit containing combination

A kit and gastric cancer technology, applied in the field of biomedicine, can solve the problems of inability to reflect the overall situation of patients, incomplete diagnosis and treatment of gastric cancer, and incomplete understanding of disease progression, so as to improve the evaluation of prognosis, avoid detection errors, and improve accuracy. sexual effect

Inactive Publication Date: 2017-12-19
NANYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Tumor is a very complex whole, and its heterogeneity has brought many problems to the accurate diagnosis and precise treatment of tumors: 1) Biopsy based on a single tumor specimen, because it cannot represent the entire tumor, is not suitable for tumor cells that have metastasized For patients, only taking tumor tissue from a certain part cannot reflect the overall situation of the patient; 2) When taking tumor samples, pathologists often focus on the worst tumor tissue, and focus on the most progressive and invasive areas. May be missed; 3) Due to the heterogeneous expression of the detection markers in the tumor, judging by one or two detection markers is more likely to lead to an incomplete understanding of the progress of the disease
[0006] In view of the current incomplete diagnosis and treatment of gastric cancer

Method used

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  • LncRNA combination for detecting prognosis condition of stomach cancer and kit containing combination
  • LncRNA combination for detecting prognosis condition of stomach cancer and kit containing combination
  • LncRNA combination for detecting prognosis condition of stomach cancer and kit containing combination

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Experimental program
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Effect test

Embodiment 1

[0060] RT-qPCR technology was used to detect the differential expression of lncRNA combinations in gastric cancer tissues and paired normal gastric mucosal tissues: 1. The total RNA of gastric cancer tissues and corresponding paracancerous tissues was extracted by nucleic acid purification column

[0061] Pretreatment: In a clean area with less RNase interference (all utensils are wiped with 1‰ DEPC water), the mortar needs to be sterilized at 200°C for 5 hours and cooled for 4 hours.

[0062] 2. Tissue Lysis:

[0063] 1) Weigh about 20 mg of isolated gastric cancer tissue and para-cancerous tissue samples into a mortar that has been poured with liquid nitrogen and pre-cooled, and grind to powder with a grinding rod (incomplete grinding will seriously affect the yield of RNA ); 2) Add 700μl QIAzol lysate to the mortar, continue to grind to a homogeneous liquid without obvious tissue pieces, and then transfer to a 1.5ml eppendorf tube without RNase; 3) Centrifuge in a refrigera...

Embodiment 2

[0078] Example 2. Using RT-qPCR technology to detect the differential expression of lncRNA combinations in gastric cancer patient serum and healthy controls:

[0079] 1. Serum RNA is extracted The present invention adopts centrifugal column type RNA rapid extraction kit to extract, and concrete steps are as follows:

[0080] 1) Take 0.25ml of serum, add 0.75ml of Lysis Buffer RLS, shake the homogenate sample vigorously, and incubate at 15-30°C for 5 minutes to completely decompose the protein body;

[0081] 2) Centrifuge at 12000rpm for 10min at 4°C, carefully take the supernatant and transfer it to a new RNase free centrifuge tube;

[0082] 3) Add 0.15ml chloroform, cover the sample tube tightly, shake vigorously for 15s and incubate at room temperature for 3min;

[0083] 4) Centrifuge at 4°C and 12000rpm for 10min, the sample will be divided into three layers: the lower layer is the organic phase, the middle layer and the upper layer are colorless aqueous phases, RNA exists...

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Abstract

The invention discloses an LncRNA combination for detecting a prognosis condition of stomach cancer and a kit containing the combination. According to the invention, a fluorescent quantitation PCR or digital PCR technique is adopted to identify the difference change of a set of specific LncRNA expression quantity in a stomach cancer patient sample (tissue, plasma, serum, gastric juice, and the like) and a corresponding paracancerous sample or normal sample and to early and accurately evaluate the risk of stomach cancer relapse or transfer. The kit disclosed by the invention provides a biomarker LncRNA combination for detecting the prognosis condition of stomach cancer, a primer for detecting the LncRNA contained in the combination and a related reagent, so that the kit is capable of effectively increasing the detection efficiency and accuracy for the stomach cancer prognosis relapse or transfer. The invention adopts a set of prognosis transfer related LncRNA combination for avoiding the defect of great increasing of the fault diagnosis rate and missed diagnosis rate of the stomach cancer diagnosis caused by lower sensitivity and specificity resulted from a detection index LncRNA served as a tumor marker kit.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an LncRNA combination for detecting the prognosis of gastric cancer and a kit containing the combination. Background technique [0002] Gastric cancer (GC) is a malignant tumor of the glandular epithelial cells of the gastric mucosa, most of which are adenocarcinomas. It is a highly heterogeneous tumor with great differences in its clinical manifestations and histological morphology. Currently, gastric cancer is one of the most common malignant tumors worldwide. Both the incidence and death of gastric cancer in my country exceed 40% of the global mortality rate. Under the condition of medical intervention, predicting the degree of short-term and long-term recovery or progression of the disease is very important for patient survival or recovery. Traditional gastric cancer prognosis prediction methods are based on clinical case characteristics such as patient age,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/113
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 徐茜阚云超李新梅黄盈盈张冰姚路路李奥琦李娜赵珂
Owner NANYANG NORMAL UNIV
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