Chronic kidney disease renal function diagnostic kit based on lncRNA-H19 and application of lncRNA-H19

Abstract

The invention relates to a chronic kidney disease renal function diagnostic kit based on lncRNA-H19 and application of the lncRNA-H19. The kit comprises an RAN extracting agent, an lncRNA-H19 primer, a reverse transcription system and a standard PCR (Polymerase Chain Reaction) system. An experiment finds out that the expression condition of the lncRNA-H19 in blood serum of a patient has positive correlation with the development of a chronic kidney disease renal function; when the expression of the lncRNA-H19 is higher, the renal function of the patient is better; after treatment, the prognosis of the patient is better when the effect is better; the lncRNA-H19 is promoted to be used as a checking standard for helping the diagnosis on the chronic kidney disease renal function, so that the lncRNA-H19 can be used as a related index for diagnosing the chronic kidney disease renal function and can be used for manufacturing a chronic kidney disease renal function diagnostic reagent and the diagnostic kit.

Description

technical field [0001] The invention belongs to the field of diagnostic reagents for chronic kidney disease, and in particular relates to a renal function diagnostic kit for chronic kidney disease based on lncRNA-H19 and the application of lncRNA-H19. Background technique [0002] Chronic kidney disease refers to renal structure and dysfunction caused by various chronic damages of more than three months, including the decline of glomerular filtration rate, renal pathological damage, abnormal blood and urine components, and abnormalities of renal imaging. abnormal. Diseases that cause chronic kidney disease mainly include various primary and secondary glomerulonephritis, as well as some renal tubular damage and renal vascular lesions. Among them, primary glomerulonephritis is one of the most important causes of chronic renal insufficiency and even uremia. In my country and even in the world, IgA nephropathy is one of the most common primary glomerular diseases, with hematur...

AI Technical Summary

Problems solved by technology

The diagnosis of chronic kidney disease mainly relies on renal biopsy, and there is no other alternative method. However, renal biopsy is risky and expensive, and it is generally only allowed to be performed once, which greatly limits the later detection of the patient's condition and response to curative effect , prognosis assessment

However, there is no relevant report on the correlation between lncRNA-H19 and nephritis

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