Chronic kidney disease renal function diagnostic kit based on lncRNA-H19 and application of lncRNA-H19
A chronic kidney disease and diagnostic kit technology, applied in the field of chronic kidney disease diagnostic reagents, can solve the problems of limiting the patient's condition, such as detection of curative effect response, prognosis evaluation, high cost, high risk of renal biopsy surgery, etc., and achieves sensitivity and specificity. The effect of good degree, convenient inspection, risk and cost reduction
Inactive Publication Date: 2016-09-28
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology
The diagnosis of chronic kidney disease mainly relies on renal biopsy, and there is no other alternative method. However, renal biopsy is risky and expensive, and it is generally only allowed to be performed once, which greatly limits the later detection of the patient's condition and response to curative effect , prognosis assessment
However, there is no relevant report on the correlation between lncRNA-H19 and nephritis
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[0022] The present invention will be described in further detail below in conjunction with specific experiments and accompanying drawings.
[0023] The present invention quantifies the expression of lncRNA-H19 in the serum of patients with chronic kidney disease such as simple IgA nephropathy, membranous nephropathy, mild mesangial proliferative glomerulonephritis and normal healthy people with different Lee's grades by qRT-PCR, and carries out Statistical comparison, registration of treatment effects, establishment of a follow-up system, and judging the role of lncRNA-H19 in the diagnosis, treatment and prognosis evaluation of CKD patients such as IgA nephropathy.
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The invention relates to a chronic kidney disease renal function diagnostic kit based on lncRNA-H19 and application of the lncRNA-H19. The kit comprises an RAN extracting agent, an lncRNA-H19 primer, a reverse transcription system and a standard PCR (Polymerase Chain Reaction) system. An experiment finds out that the expression condition of the lncRNA-H19 in blood serum of a patient has positive correlation with the development of a chronic kidney disease renal function; when the expression of the lncRNA-H19 is higher, the renal function of the patient is better; after treatment, the prognosis of the patient is better when the effect is better; the lncRNA-H19 is promoted to be used as a checking standard for helping the diagnosis on the chronic kidney disease renal function, so that the lncRNA-H19 can be used as a related index for diagnosing the chronic kidney disease renal function and can be used for manufacturing a chronic kidney disease renal function diagnostic reagent and the diagnostic kit.
Description
technical field [0001] The invention belongs to the field of diagnostic reagents for chronic kidney disease, and in particular relates to a renal function diagnostic kit for chronic kidney disease based on lncRNA-H19 and the application of lncRNA-H19. Background technique [0002] Chronic kidney disease refers to renal structure and dysfunction caused by various chronic damages of more than three months, including the decline of glomerular filtration rate, renal pathological damage, abnormal blood and urine components, and abnormalities of renal imaging. abnormal. Diseases that cause chronic kidney disease mainly include various primary and secondary glomerulonephritis, as well as some renal tubular damage and renal vascular lesions. Among them, primary glomerulonephritis is one of the most important causes of chronic renal insufficiency and even uremia. In my country and even in the world, IgA nephropathy is one of the most common primary glomerular diseases, with hematur...
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Login to View More IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 何丽洁景蕊张晓敏胡晋平车明文石田田赵丽娟娄未娟
Owner FOURTH MILITARY MEDICAL UNIVERSITY