A method for determining the mouse bioavailability of nickel in soil and food

Abstract

The invention discloses a method for measuring the biological availability of nickel in soil and food in mice. The specific steps are to make food containing nickel to feed the mice, and collect the urine of the mice to finally obtain the mice containing nickel in soil and food. Relative to the bioavailability, the present invention improves and innovates on conventional experimental methods, and designs a method that can accurately and effectively measure the bioavailability of nickel in soil and food in mice, that is, the end point of nickel accumulation is located in the urine, through The urine of the mice was collected, and the nickel content in the urine was measured to obtain the amount of nickel absorbed by the mice, so as to calculate the relative biological availability of nickel in the mice.

Description

technical field [0001] The invention relates to the field of environmental science, in particular to a method for measuring the biological availability of nickel in soil and food in mice. Background technique [0002] The human health risk assessment of nickel in soil and food is usually assessed by the bioavailability of nickel. The human bioavailability of nickel refers to the ratio of the amount of nickel absorbed by the human body to the total intake of nickel, and this ratio is ≤100%. The methods for determining the biological effectiveness are divided into two types: animal experiments and in vitro simulation experiments. In vitro simulation experiments have the advantages of simplicity and convenience, but the results usually have large errors, making it difficult to simulate the real situation of the human body. Live animal experiments have a long cycle and complicated operations, but they can more accurately reflect the biological effectiveness of the animal body ...

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