A method for determining the mouse bioavailability of nickel in soil and food

A bioavailability and mouse technology, applied in the field of environmental science, can solve the problems of inability to measure the bioavailability and nickel accumulation of mice

Active Publication Date: 2020-12-15
NANJING UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, if the conventional mouse experiment method is used to determine the mouse bioavailability of nickel in soil and food, the final target organs indicating nickel accumulation are defined as the liver and kidney. Accumulated in the kidney, bioavailability in mice could not be determined

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  • A method for determining the mouse bioavailability of nickel in soil and food
  • A method for determining the mouse bioavailability of nickel in soil and food
  • A method for determining the mouse bioavailability of nickel in soil and food

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Embodiment Construction

[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0023] In an embodiment of the present invention, a method for measuring the bioavailability of nickel in soil and food in mice, the specific steps are as follows:

[0024] (1) Add soluble nickel reference material (nickel sulfate, NiSO4) to foods with low nickel concentration to facilitate the production of a certain concentration range of nickel (0, 0.5, 1.0, 1.5 and 2.5mg Ni Kg -1 ) standard curve;

[0025] (2) Group the mice according to the food with different concentra...

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Abstract

The invention discloses a method for measuring the biological availability of nickel in soil and food in mice. The specific steps are to make food containing nickel to feed the mice, and collect the urine of the mice to finally obtain the mice containing nickel in soil and food. Relative to the bioavailability, the present invention improves and innovates on conventional experimental methods, and designs a method that can accurately and effectively measure the bioavailability of nickel in soil and food in mice, that is, the end point of nickel accumulation is located in the urine, through The urine of the mice was collected, and the nickel content in the urine was measured to obtain the amount of nickel absorbed by the mice, so as to calculate the relative biological availability of nickel in the mice.

Description

technical field [0001] The invention relates to the field of environmental science, in particular to a method for measuring the biological availability of nickel in soil and food in mice. Background technique [0002] The human health risk assessment of nickel in soil and food is usually assessed by the bioavailability of nickel. The human bioavailability of nickel refers to the ratio of the amount of nickel absorbed by the human body to the total intake of nickel, and this ratio is ≤100%. The methods for determining the biological effectiveness are divided into two types: animal experiments and in vitro simulation experiments. In vitro simulation experiments have the advantages of simplicity and convenience, but the results usually have large errors, making it difficult to simulate the real situation of the human body. Live animal experiments have a long cycle and complicated operations, but they can more accurately reflect the biological effectiveness of the animal body ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50G01N27/62
CPCG01N33/5038G01N27/62
Inventor 王珏旸历红波
Owner NANJING UNIV
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