Construction method and application of megacolon animal model

A construction method and technology of animal models, applied in the field of construction of Hirschsprung animal models, can solve the problems of infection, complicated operation, low survival rate, etc., and achieve the effect of rich diversity, reduced operation difficulty, and high success rate of modeling

Active Publication Date: 2022-04-08
GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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Problems solved by technology

However, benzalkonium chloride laparotomy and encircling the colon are prone to postoperative infection, intestinal stenosis caused by tissue adhesion around the intestine, low survival rate, low success rate of modeling, and complicated operation

Method used

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  • Construction method and application of megacolon animal model
  • Construction method and application of megacolon animal model
  • Construction method and application of megacolon animal model

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Experimental program
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Embodiment 1

[0060] 1. Preparation of injection tools:

[0061] Type I is used for perfusion at the age of 5-8 days and 12-15 days. Choose 0.3mm, 0.5mm, and 0.6mm tetrafluorocapillary tubes with an outer diameter of 2cm each, and insert them in order according to the thickness of the outer diameter, from thin to thick. The 0.6mm outer diameter end is then connected to the No. 6 needle (outer diameter 0.6mm) such as figure 1 a and figure 2 As shown in a, connect a 1 ml syringe.

[0062] Type II, used for perfusion in 19-21 days old: select the PTFE capillary with an outer diameter of 0.5mm and 0.6mm and connect it according to the method of Type I, such as figure 1 b and figure 2 as shown in b.

[0063] 2. Drug concentration optimization:

[0064] In terms of injection concentration, the highest concentration of benzalkonium chloride under low lethality was determined to be the optimal experimental concentration through optimization. As shown in Table 1, the first and second optimal ...

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Abstract

The invention discloses a construction method and application of a megacolon animal model. Specifically, benzalkonium chloride is injected into mice at 5-8 days old, 12-15 days old and 19-21 days old through anus perfusion. According to the method, the BALB / C suckling mouse is used for modeling the megacolon model for the first time, clysis is used as a means, surgical operation is not needed, the operation difficulty is reduced, the modeling success rate is high, the BALB / C suckling mouse of 5-8 days old is selected as an experimental subject, benzalkonium chloride anus perfusion is carried out under the condition that the intestinal tract development is not mature, intestinal neurons are damaged, and the method is closer to the HSCR attack microenvironment; the method is more suitable for clinical HSCR diagnosis and treatment age, and is beneficial to HSCR pathological mechanism research and experimental optimization and evaluation of novel treatment means. And a good research model is provided for clinical HSCR pathological mechanisms and further diagnosis and treatment. And the diversity of the megacolon animal model is enriched.

Description

technical field [0001] The invention belongs to the technical field of animal model construction, and in particular relates to a method for constructing an animal model of Hirschsprung's colon and its application. Background technique [0002] Hirschsprung’s disease (HSCR) is a common digestive and developmental malformation in children, with an incidence rate of 1 / 5000 and a male to female ratio of 4:1. The main cause of the disease is the long-distance migration and abnormal development of neural crest cells (neural crest cells, NCCs) during the embryonic period, resulting in the absence of ganglion cells of different lengths in the distal colon. Due to the absence of ganglion cells, the distal colon loses the functions of peristalsis and defecation, and is in a state of spasm, contraction and stenosis. The proximal colon accumulates feces and gas, and then expands and hypertrophies, forming megacolon lesions. Surgical resection of diseased bowel is currently the main tre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A61D7/00
Inventor 蓝超婷张彦朱云吴裕鑫夏慧敏
Owner GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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