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Kits, methods and applications for highly sensitive detection of T cells and their functions

A kit and functional technology, applied in the field of kits for high-sensitivity detection of T cells and functions, can solve problems such as failure to achieve detection rate, hinder disease process evaluation and curative effect evaluation, and inability to distinguish T cell immune status, and achieve enhanced detection. The effect of yield

Active Publication Date: 2019-03-05
深圳中恒祥生物免疫技术有限公司
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Problems solved by technology

However, none of the above methods can achieve a good detection rate and cannot distinguish the immune status of T cells in patients, thus hindering the evaluation of disease process and efficacy evaluation

Method used

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  • Kits, methods and applications for highly sensitive detection of T cells and their functions
  • Kits, methods and applications for highly sensitive detection of T cells and their functions
  • Kits, methods and applications for highly sensitive detection of T cells and their functions

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Embodiment Construction

[0025] The kit for highly sensitive detection of the quantity and function of specific T cells and the specific detection method of the present invention are introduced in detail below.

[0026] The present invention uses a high-sensitivity detection kit for the number and function of T cells, which includes: a microporous filter membrane double-chamber culture dish, a culture medium for a double-chamber filter system, T cell culture fluid, coated with anti-human IFN- ELISPOT culture plate of γ, TNF-α, IL-2, CD4, CD8 monoclonal antibody and 96-well plate of cellulose membrane, mouse anti-human CD3 monoclonal antibody solution, AP enzyme-labeled streptavidin, and chromogenic solution .

[0027] Use the above kit to detect T cells in 100 cases of collected fresh peripheral blood, the steps are as follows:

[0028] 1. Assemble the microporous membrane double-chamber culture system:

[0029] A Transwell chamber with a microporous membrane with a pore size of 8 μm to 12 μm (prefe...

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Abstract

The invention provides a kit for highly-sensitively detecting the number and the functions of T cells. The kit comprises a micro-porous filter membrane double-chamber culture dish, a culture medium for a double-chamber filter membrane system, a T cell culture solution, a 96-well plate coated with an ELISPOT culture plate of anti-human IFN-gamma, TNF-alpha, IL-2, CD4 and CD8 monoclonal antibodies and a cellulose membrane, a mouse anti-human CD3 monoclonal antibody solution, AP enzyme-labeled streptavidin, and a chromogenic solution. The invention also provides a method for detecting the T cells. The kit and the method are capable of highly-efficiently detecting the T cells.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a kit for highly sensitive detection of T cells and their functions, a detection method, and an application thereof. Background technique [0002] In recent years, research in the field of biomedicine has made it possible to cure some cancers and chronic infectious diseases. Among them, T cell-related immunotherapy has become a star strategy, which activates T cells, especially specific T cells, through various ways to make them Wander around and inspect, and secrete effector cytokines IFN-γ, TNF-α, IL-2 to find tumors or pathogens to play anti-cancer and anti-infection effects. Therefore, T cell therapy has huge potential and market. Theoretically, T cells need to reach a certain sufficient number to be effective; the evaluation of curative effect is the key to the feasibility and continuous optimization of treatment strategies. [0003] Due to the inhibitory internal e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577C12Q1/06
CPCG01N33/505G01N33/577
Inventor 黄月华
Owner 深圳中恒祥生物免疫技术有限公司
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