Kit and method for highly-sensitively detecting T cells and functions thereof, and application of kit

A kit and functional technology, applied in the field of kits for high-sensitivity detection of T cells and functions, can solve problems such as failure to achieve detection rate, hinder disease process evaluation and curative effect evaluation, and inability to distinguish T cell immune status, and achieve enhanced detection. The effect of yield

Active Publication Date: 2017-10-13
深圳中恒祥生物免疫技术有限公司
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Problems solved by technology

However, none of the above methods can achieve a good detection rate and cannot distinguish the immune status of T cells in patients, thus hindering the evaluation of disease process and efficacy evaluation

Method used

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  • Kit and method for highly-sensitively detecting T cells and functions thereof, and application of kit
  • Kit and method for highly-sensitively detecting T cells and functions thereof, and application of kit
  • Kit and method for highly-sensitively detecting T cells and functions thereof, and application of kit

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Embodiment Construction

[0025] The following describes in detail the high-sensitivity kit for detecting the number and function of specific T cells and specific detection methods of the present invention.

[0026] The present invention uses a high-sensitivity kit for detecting the number and function of T cells, which includes: a microporous filter double-chamber culture dish, a medium for a double-compartment filter system, a T cell culture solution, and an anti-human IFN-coated γ, TNF-α, IL-2, CD4, CD8 monoclonal antibody ELISPOT culture plate and cellulose membrane 96-well plate, mouse anti-human CD3 monoclonal antibody solution, AP enzyme-labeled streptavidin, and color developing solution .

[0027] Use the above kit to detect 100 cases of T cells in fresh peripheral blood, the steps are as follows:

[0028] 1. Assemble a dual-chamber culture system with microporous membranes:

[0029] A Transwell cell with a microporous membrane with a pore size of 8 μm to 12 μm (preferably 8 μm) is used, placed in a ...

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Abstract

The invention provides a kit for highly-sensitively detecting the number and the functions of T cells. The kit comprises a micro-porous filter membrane double-chamber culture dish, a culture medium for a double-chamber filter membrane system, a T cell culture solution, a 96-well plate coated with an ELISPOT culture plate of anti-human IFN-gamma, TNF-alpha, IL-2, CD4 and CD8 monoclonal antibodies and a cellulose membrane, a mouse anti-human CD3 monoclonal antibody solution, AP enzyme-labeled streptavidin, and a chromogenic solution. The invention also provides a method for detecting the T cells. The kit and the method are capable of highly-efficiently detecting the T cells.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a kit, a detection method, and applications for high-sensitivity detection of T cells and functions. Background technique [0002] In recent years, research in the field of biomedicine has made it possible to cure some cancers and chronic infectious diseases. Among them, T cell-related immunotherapy has become a star strategy, which activates T cells, especially specific T cells, through a variety of ways to make them in the body Wandering and patrolling, and secreting the effective cytokines IFN-γ, TNF-α, IL-2 to find tumors or pathogens to play anti-cancer and anti-infection effects, therefore, T cell therapy has great potential and market. Theoretically, T cells need to reach a certain number of sufficient numbers to be effective; the evaluation of curative effect is the key to the feasibility and continuous optimization of treatment strategies. [0003] Due to the inhibitory...

Claims

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Application Information

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IPC IPC(8): G01N33/577C12Q1/06
CPCG01N33/505G01N33/577
Inventor 黄月华
Owner 深圳中恒祥生物免疫技术有限公司
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