Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for determining biological availability of mice with nickel in soil and food

A bioavailability and mouse technology, applied in the field of environmental science, can solve the problems of nickel accumulation and inability to measure the bioavailability of mice

Active Publication Date: 2020-04-03
NANJING UNIV
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, if the conventional mouse experiment method is used to determine the mouse bioavailability of nickel in soil and food, the final target organs indicating nickel accumulation are defined as the liver and kidney. Accumulated in the kidney, bioavailability in mice could not be determined

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining biological availability of mice with nickel in soil and food
  • Method for determining biological availability of mice with nickel in soil and food
  • Method for determining biological availability of mice with nickel in soil and food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0023] In an embodiment of the present invention, a method for measuring the bioavailability of nickel in soil and food in mice, the specific steps are as follows:

[0024] (1) Add soluble nickel reference material (nickel sulfate, NiSO4) to foods with low nickel concentration to facilitate the production of standards with a certain concentration range of nickel (0, 0.5, 1.0, 1.5 and 2.5mg Ni kg-1) curve;

[0025] (2) Group the mice according to the food with different conce...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for determining the biological availability of a mouse with nickel in soil and food. The method comprises the following specific steps: preparing nickel-containing food for feeding mice; collecting mouse urine, finally, acquiring the relative biological effectiveness of the mice with nickel in soil and food. The method is improved and innovated on the basis of a conventional experimental method. A method capable of accurately and effectively measuring the biological effectiveness of the mouse with nickel in soil and food is designed, namely, the end point of nickel accumulation is positioned in urine, the amount of nickel absorbed by the mouse is obtained by collecting the urine of the mice and measuring the content of nickel in the urine, and therefore therelative biological effectiveness of the mice with nickel is calculated.

Description

technical field [0001] The invention relates to the field of environmental science, in particular to a method for measuring the biological availability of nickel in soil and food in mice. Background technique [0002] The human health risk assessment of nickel in soil and food is usually assessed by the bioavailability of nickel. The human bioavailability of nickel refers to the ratio of the amount of nickel absorbed by the human body to the total intake of nickel, and this ratio is ≤100%. The methods for determining the biological effectiveness are divided into two types: animal experiments and in vitro simulation experiments. In vitro simulation experiments have the advantages of simplicity and convenience, but the results usually have large errors, making it difficult to simulate the real situation of the human body. Live animal experiments have a long cycle and complicated operations, but they can more accurately reflect the biological effectiveness of the animal body ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N27/62
CPCG01N33/5038G01N27/62
Inventor 王珏旸历红波
Owner NANJING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com