Cell circular scar healing experimental device for six-pore plate

An experimental device and cell technology, which is applied in the field of cell biology, can solve the problems of scratching culture vessels, affecting experimental results, and large experimental errors, so as to maintain cell activity, save experimental time, and save experimental costs.

An experimental device and cell technology, which is applied in the field of cell biology, can solve the problems of scratching culture vessels, affecting experimental results, and large experimental errors, so as to maintain cell activity, save experimental time, and save experimental costs.

CN109055218APending Publication Date: 2018-12-21CHILDRENS HOSPITAL OF CHONGQING MEDICAL UNIV
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  • Cell circular scar healing experimental device for six-pore plate
  • Cell circular scar healing experimental device for six-pore plate
  • Cell circular scar healing experimental device for six-pore plate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A cell wound healing experimental device suitable for a six-hole plate, comprising a sheet (1), the top surface of the sheet is provided with a cross structure (2), and the bottom surface of the sheet (1) is provided with a cylinder (3) , the cylinder (3) is correspondingly arranged below the cross end (6) of the cross structure; the sheet is circular, and the intersection point (5) of the cross structure (2) is located at the center of the circular sheet ; The cross structure is length (L) 3.48cm, height (H) 1.0cm, thickness (T) 0.2cm; diameter (D) of the circular sheet is 1.88cm, thickness (T) 0.1cm; The cylinder (3) has a height (H) of 0.3 cm and a bottom diameter (D) of 0.2 cm; the disc (1) is fixed to the cross structure (2), and the cylinder (3) is fixed to the disc ( 1); there are 4 cylindrical structures (3), which are respectively located under the 4 cross ends (6) of the cross structure (2); the sheet (1) and the cross structure (2), cylindrical structure (3)...

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Abstract

The invention provides a cell circular scar healing experimental device for a six-pore plate. The device comprises a flake (1), wherein a criss-cross structure (2) is arranged at the bottom of the flake; a cylindrical structure (3) is arranged at the bottom of a cross end of the criss-cross structure. The device is convenient for observing scar healing in the whole visual field, and avoids the phenomenon that only local area healing situation of a scar can be observed in existing cell scar experiments.

Description

technical field [0001] The invention relates to the field of cell biology and discloses a cell wound healing experimental device for preparing circular wounds. technical background [0002] Cell wound healing assay is currently the most commonly used in vitro experimental method to study cell migration ability. The basic principle is: when the cells grow and fuse to a monolayer state, a "scar" is artificially created on the monolayer cultured cells, and the cells at the edge will gradually enter the blank area to heal the "scratch". By calculating the healing rate of cell scars and evaluating the migration ability of cells, the process of cell migration in vivo is simulated to a certain extent. Factors that alter cell migration and / or growth in vitro can increase or decrease wound healing rates, so this assay is often used to investigate the role of various experimental conditions such as gene regulation and drugs in cell migration. The experiment is simple and cheap. [...

Claims

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Application Information

Patent Timeline
21 Dec 2018
Publication
CN109055218A
IPC
C12M3/00; C12M1/00
CPC
C12M23/12
Inventors
毕杨; 何懿晨