Cell circular scar healing experimental device for six-pore plate

An experimental device and cell technology, which is applied in the field of cell biology, can solve the problems of scratching culture vessels, affecting experimental results, and large experimental errors, so as to maintain cell activity, save experimental time, and save experimental costs.

Pending Publication Date: 2018-12-21
CHILDRENS HOSPITAL OF CHONGQING MEDICAL UNIV
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  • Abstract
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Problems solved by technology

First of all: artificial "scratches" are difficult to ensure consistency, the experimental error is large, there are many interference factors, and manual scratches may scratch the coating on the surface of the culture vessel, thereby affecting the experimental results
Secondly, the cell wound healing experiment requires continuous observation to evaluate the effects of treatment factors on cell migration. The traditional method is to pre-mark the observation position on the back of the culture plate, but the cell growth cannot be predicted before the experiment. Each observation under the microscope It is impossible to be completely consistent, and the traditional method calculates the migration distance of a certain part, and can only obtain the data of local cell migration

Method used

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  • Cell circular scar healing experimental device for six-pore plate
  • Cell circular scar healing experimental device for six-pore plate
  • Cell circular scar healing experimental device for six-pore plate

Examples

Experimental program
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Embodiment 1

[0026] A cell wound healing experimental device suitable for a six-hole plate, comprising a sheet (1), the top surface of the sheet is provided with a cross structure (2), and the bottom surface of the sheet (1) is provided with a cylinder (3) , the cylinder (3) is correspondingly arranged below the cross end (6) of the cross structure; the sheet is circular, and the intersection point (5) of the cross structure (2) is located at the center of the circular sheet ; The cross structure is length (L) 3.48cm, height (H) 1.0cm, thickness (T) 0.2cm; diameter (D) of the circular sheet is 1.88cm, thickness (T) 0.1cm; The cylinder (3) has a height (H) of 0.3 cm and a bottom diameter (D) of 0.2 cm; the disc (1) is fixed to the cross structure (2), and the cylinder (3) is fixed to the disc ( 1); there are 4 cylindrical structures (3), which are respectively located under the 4 cross ends (6) of the cross structure (2); the sheet (1) and the cross structure (2), cylindrical structure (3)...

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Abstract

The invention provides a cell circular scar healing experimental device for a six-pore plate. The device comprises a flake (1), wherein a criss-cross structure (2) is arranged at the bottom of the flake; a cylindrical structure (3) is arranged at the bottom of a cross end of the criss-cross structure. The device is convenient for observing scar healing in the whole visual field, and avoids the phenomenon that only local area healing situation of a scar can be observed in existing cell scar experiments.

Description

technical field [0001] The invention relates to the field of cell biology and discloses a cell wound healing experimental device for preparing circular wounds. technical background [0002] Cell wound healing assay is currently the most commonly used in vitro experimental method to study cell migration ability. The basic principle is: when the cells grow and fuse to a monolayer state, a "scar" is artificially created on the monolayer cultured cells, and the cells at the edge will gradually enter the blank area to heal the "scratch". By calculating the healing rate of cell scars and evaluating the migration ability of cells, the process of cell migration in vivo is simulated to a certain extent. Factors that alter cell migration and / or growth in vitro can increase or decrease wound healing rates, so this assay is often used to investigate the role of various experimental conditions such as gene regulation and drugs in cell migration. The experiment is simple and cheap. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/00
CPCC12M23/12
Inventor 毕杨何懿晨梁小华方姝煜龚梦嘉
Owner CHILDRENS HOSPITAL OF CHONGQING MEDICAL UNIV
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