Human TSHR extracellular fusion protein and preparation method thereof

A fusion protein, extracellular segment technology, applied in biochemical equipment and methods, chemical instruments and methods, microorganism-based methods, etc., can solve problems such as low TSH binding rate
CN101928348AInactive Publication Date: 2010-12-29THE FIRST AFFILIATED HOSPITAL OF MEDICAL COLLEGE OF XIAN JIAOTONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
THE FIRST AFFILIATED HOSPITAL OF MEDICAL COLLEGE OF XIAN JIAOTONG UNIV
Publication Date
2010-12-29
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses human TSHR extracellular fusion protein and a preparation method thereof. The N end of the human TSHR extracellular fusion protein is connected with exogenous secretion signal peptide, and the C end thereof is connected with a purification marker sequence; when the fusion protein is expressed and purified, the fusion protein the N end of which is connected with the exogenous secretion signal peptide is resected, and the specific amino acid sequence is shown in SEQ ID No.1. The invention obtains high-expression soluble recombinant protein with the antigenic secretion form of TSH receptor in a good in-vitro expression system by constructing recombinant pichia pastoris containing pPICZ alpha A-hTSHRecd expression vector.
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Description

technical field

[0001] The invention belongs to the field of biotechnology and relates to the recombination and bioengineering preparation of human TSH receptors, in particular to a human TSHR extracellular fusion protein and a preparation method thereof. Background technique

[0002] Autoimmune thyroid disease (AITD) is an organ-specific disease, including Graves' disease (GD), Hashimoto's thyroiditis (Hashimoto's thyroiditis, HT) and idiopathic myxedema (Primary myxedema, PM), its incidence rate accounts for the first place in endocrine system diseases. Thyrotropin receptor (TSHR) is the main antigen that induces AITD.

[0003] The molecular weight of human TSHR is 84.5KD, and it reaches 100KD after glycosylation, which is closely related to the pathogenesis of AITD. Human TSHR mainly exists on the thyroid cell membrane and belongs to one of the members of the guanine nucleotide regulatory protein (G protein) coupled receptor superfamily; it consists of three parts: the ...

Claims

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