35 results about "Thyrotropin receptor" patented technology

A method of detecting thyroid cancer in a subject includes obtaining a nucleic acid sample from a bodily sample of the subject and determining whether the nucleic acid sample contains at least one of thyroid stimulating hormone receptor (TSHR) mRNA or thyroglobulin (Tg) mRNA.

The invention discloses the expressing product of human thyrotropin receptor extracellular domain amino terminus gene and its preparation methods and the application of the product in the enzyme immunoassay technology. The purpose of this invention is to divide TSHR-ecd cDNA into three regions(hTSHR-N terminal, hTSHR-C terminal and hTSHR-M terminal), clone them respectively, especially the hTSHR-N terminal, construct expression vector, express in E.coli, and establish TRAb (including TSAb. TSBAb) ELISA technology taking the protein of this gene engineering fragmentas antigen. The said invention develops novel channel for further research of TSHR antigen determinant and settles foundation for the ultimate transformation into TSAb, TSBAb clinical diagnostic kit.

The invention relates to a human thyrotropin receptor antibody chemiluminescence detection kit as well as a preparation method and an application method thereof, and belongs to the technical field ofimmunoassay medical detection. The method can be used for solving the problems that an existing immunoassay method for detecting the human thyrotropin receptor antibody cannot distinguish stimulating,inhibition and neutralizing antibodies of human thyrotropin receptors, and the detection sensitivity and accuracy are poor. The kit disclosed by the invention uses a streptavidin-biotin system at thecombinational ratio of streptavidin to biotin of 1:4, so that the signal is further amplified, and the reagent sensitivity is improved. Specificity detection of the stimulating antibodies of the thyrotropin receptor is carried out by changing the marked antibody and the antigen, so that detection specificity is enhanced and more accurate and more targeted in-vitro diagnosis reagents are providedfor clinical diagnosis. The kit adopts a one-step competition method reaction mode, and the content of the stimulating antibodies of the thyrotropin receptor in human serum is quantitatively detected,so that the sensitivity and specificity of detection are ensured.

The invention discloses the expressing product of human thyrotropin receptor extracellular domain carboxy-terminal gene and its preparation methods and the application of the product in the enzyme immunoassay technology. The purpose of this invention is to divide TSHR-ecd cDNA into three regions(hTSHR-N terminal, hTSHR-C terminal and hTSHR-M terminal), clone them respectively, especially the hTSHR-C terminal, construct expression vector, express in E.coli, and establish TRAb (including TSAb. TSBAb) ELISA technology taking the protein of this gene engineering fragmentas antigen. The said invention develops novel channel for further research of TSHR antigen determinant and settles foundation for the ultimate transformation into TSAb, TSBAb clinical diagnostic kit.

The invention provides an isolated antibody for the TSHR which is an antagonist of TSH. The invention also relates to methods of using antibodies of the invention.

The invention relates to a bridge assay that can be used on an automatic diagnostic apparatus in order to detect anti-thyrotropin receptor autoantibodies, wherein the chimeric TSH receptor used comprises the extracellular portion of the chimeric TSH receptor and is N-terminally fused to a protein causing secretion of the chimeric TSH receptor from culture cells, and the anchored chimeric TSH receptor is immobilized on paramagnetic particles.

The invention discloses an enzyme-linked immunoassay kit and a detection method capable of simultaneously detecting thyroid stimulating hormone receptor subtype antibodies. The kit comprises a coatingantigen, a precoated ELISA plate, a rinsing solution, a thyroid stimulating hormone receptor antibody standard, an ELISA solution, a diluted sample solution, a rinsing solution, a blocking solution,a color developing solution and a stop solution. The detection method comprises the following steps: coating, washing, adding a sample, washing, developing the color and stopping. The kit is high in sensitivity, good in specificity and easy to operate, and can be used for quantitatively detecting the concentrations of the thyroid stimulating hormone receptor subtype antibodies; a result obtained after detection through use of the kit and the detection method has an important clinical reference value; the kit is low in price, is convenient for clinical wide-range popularization, and provides aprecise reference for a clinician to diagnose diseases.

The invention provides an isolated antibody for the TSHR which is an antagonist of TSH. The invention also relates to methods of using antibodies of the invention.

Improved receptor assays for the detection of thyroid stimulating hormone receptor (TSHR) autoantibodies are described which use immobilized, affinity-purified rTSHR preparations as specific binders. This format, as well as novel measures for neutralizing pathologically increased human TSH (hTSH) levels in the sera, e.g., by the addition of anti-hHSH antibody, and/or eliminating the influence of anti-bovine TSH antibody, result in increased assay reliability and open up the possibility of preparing the assay constituents in a ready to use and/or well-standardized form for automatic processing and/or convenient marketing.

The invention provides a kit for detecting TSHR (thyroid stimulating hormone receptor) gene mRNA expression through a fluorescence quantitation RT-PCR (real-time polymerase chain reaction) and a use method of the kit. The kit detects the existence of expression of TSHR-mRNA in peripheral blood, tumor tissue samples and the like and the expression level with a Taqman-qPCR method. The kit has the advantages of high sensitivity, high accuracy, high detection rate and specificity, is matched with an internal standard and a positive reference substance, can effectively eliminate false positive and false negative results, is high in anti-jamming capacity and has the characteristics that detection is quick and economical, trauma of patients is small and the product stability is high.

The present invention provides a peptide at least partially derivable from human Thyroid Stimulating Hormone Receptor (TSHR) which peptide is capable of binding to an MHC molecule in vitro and being presented to a T cell without further antigen processing. The present invention also relates to the use of such peptides for the prevention or suppression of activating autoantibody formation in Graves' Disease.

The invention relates to the technical field of biology, in particular to a human thyroid stimulating hormone receptor fusion protein and a preparation method and application thereof. The fusion protein is composed of a HER2 protein and a TSHR protein, and the HER2 protein and the TSHR protein are connected through a connecting peptide. The HER2 and the low-expression TSHR are subjected to fusionexpression, the yield and stability of the TSHR can be improved under the condition that the structure and function of the TSHR are not affected, the TSHR protein can be secreted into supernate, and purification is facilitated.

The invention discloses a construction method of a gene immune induced Graves' Ophthalmopathy animal model and application of a rapamycin type medicine. The construction method comprises the followingsteps: 1) diluting a mother liquid of an adenovirus expressing a thyroid-stimulating hormone receptor by using PBS (phosphate buffer saline) according to a volume ratio of 1:50 so as to obtain an Ad-TSHR (adenovirus-thyroid-stimulating hormone receptor) diluted liquid; 2) on the first day of a first week, respectively injecting 25[mu] L of the Ad-TSHR diluted liquid into left gluteus maximus muscle and right gluteus maximus muscle of experiment animals; and 3) repeating operation of the step 2) at the 3rd, 10th, 14th, 18th, 22nd, 26th and 30th weeks till the experiment animals are induced tohave Graves' Ophthalmopathy, so as to successfully construct the gene immune induced Graves' Ophthalmopathy animal model. The construction method makes up the blank of the field of Graves' Ophthalmopathy models, and is a novel exploration on rapamycin in treating Graves' Ophthalmopathy.

The invention relates to the technical field of immunoassay, in particular to a freeze-drying auxiliary preparation and a freeze-drying method of a thyroid stimulating hormone receptor. The freeze-drying auxiliary preparation is prepared from non-reducing disaccharide, glycine, serum albumin, polyethylene glycol and a buffer solution, the content of each component is as follows: 2wt%-20wt% of non-reducing disaccharide, 5-30 mmol/L of glycine, 2wt%-10wt% of serum albumin, 0.5wt%-2wt% of polyethylene glycol, and the balance being buffer. The freeze-drying auxiliary preparation of the thyroid stimulating hormone receptor provided by the invention can effectively assist in freeze-drying of the thyroid stimulating hormone receptor, so that the thyroid stimulating hormone receptor is stable in form after freeze-drying, good in freeze-drying redissolution effect, free of precipitation and free of influence on biological activity.