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Enzyme-linked immunoassay kit and detection method capable of simultaneously detecting thyroid stimulating hormone receptor subtype antibodies

A technology of thyroid-stimulating hormone and enzyme-linked immunosorbent reagents, which is applied in the field of enzyme-linked immunosorbent detection, and achieves the effect of simple operation, low price and good specificity

Inactive Publication Date: 2018-11-06
成都和同易创生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is no clinically approved diagnostic kit for TSAb and TBAb available

Method used

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Examples

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Embodiment 1

[0038] An enzyme-linked immunosorbent assay kit capable of simultaneously detecting thyroid-stimulating hormone receptor typing antibodies, including: coated antigen, pre-coated microtiter plate, washing solution, thyroid-stimulating hormone receptor antibody standard, enzyme-labeled solution, sample diluent , blocking solution, chromogenic solution and stop solution.

[0039] Among them, the sample diluent is 5-50mM, carbonate buffer solution with pH=9.6; the blocking solution is 20mM phosphate buffer solution containing 5wt%-25wt% bovine serum, pH=9.0; the enzyme standard solution is horseradish peroxidized Phytostimulating hormone receptor recombinant antigen labeled with biozyme; stop solution is 1-3M concentrated sulfuric acid.

[0040] The color-developing solution is a mixture of color-developing solution A and color-developing solution B at a volume ratio of 1:1; wherein, 1L of color-developing solution A includes 0.1-0.25M sodium acetate trihydrate, 0.5-5w / v (g / L)% ci...

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PUM

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Abstract

The invention discloses an enzyme-linked immunoassay kit and a detection method capable of simultaneously detecting thyroid stimulating hormone receptor subtype antibodies. The kit comprises a coatingantigen, a precoated ELISA plate, a rinsing solution, a thyroid stimulating hormone receptor antibody standard, an ELISA solution, a diluted sample solution, a rinsing solution, a blocking solution,a color developing solution and a stop solution. The detection method comprises the following steps: coating, washing, adding a sample, washing, developing the color and stopping. The kit is high in sensitivity, good in specificity and easy to operate, and can be used for quantitatively detecting the concentrations of the thyroid stimulating hormone receptor subtype antibodies; a result obtained after detection through use of the kit and the detection method has an important clinical reference value; the kit is low in price, is convenient for clinical wide-range popularization, and provides aprecise reference for a clinician to diagnose diseases.

Description

technical field [0001] The invention belongs to the technical field of ELISA detection, and in particular relates to an ELISA kit and a detection method capable of simultaneously detecting thyroid-stimulating hormone receptor typing antibodies. Background technique [0002] Autoimmune thyroid disease (autommune thyroid disease, AITD) is a group of complex organ-specific autoimmune diseases, patients have a variety of thyroid-specific antibodies, among which thyroid stimulating hormone receptor antibody (TRAb) is related to the occurrence of the disease, There is an important relationship between development and curative effect. TSAb can be detected in almost 90% of GD patients. TSBAb has an important relationship with the occurrence of hypothyroidism. TSBAb can inhibit TSHR and lead to hypothyroidism. [0003] Thyroid-stimulating hormone receptor (TSHR) exists on the membrane of thyroid follicle cells. Under normal circumstances, thyroid-stimulating hormone receptor (TSHR)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/535
CPCG01N33/535G01N33/6854G01N2800/046
Inventor 兰长贵张涛潘克俭李玉璞史育红
Owner 成都和同易创生物科技有限公司
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